Reviewed, UniProtKB/Swiss-Prot Q6NQB7 (B3GT7_ARATH)

Names and origin

Protein names

Recommended name:
    Beta-1,3-galactosyltransferase 7
    EC=2.4.1.-

Gene names

Name:	B3GALT7
Ordered Locus Names:	At1g77810
ORF Names:	F28K19.2, T32E8.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal glycosyl residue. Ref.4
Cofactor	Manganese Probable.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein. Ref.4
Tissue specificity	Expressed in leaves, stems, flowers and siliques. Ref.4
Sequence similarities	Belongs to the glycosyltransferase 31 family.
Sequence caution	The sequence AAF17702.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAG51626.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Ref.4 Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-1,3-galactosyltransferase activity Ref.4 Inferred from direct assay. Source: TAIR manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6NQB7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6NQB7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     86-88: Missing.
     260-266: NLLNCFR → K

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Beta-1,3-galactosyltransferase 7		PRO_0000359417
Regions
	Transmembrane	9 – 29	21	Signal-anchor for type II membrane protein Potential
Natural variations
	Alternative sequence	86 – 88	3	Missing in isoform 2.		VSP_036146
	Alternative sequence	260 – 266	7	NLLNCFR → K in isoform 2.		VSP_036147

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 0F530626D3DE7D2C	FASTA	393	44,675
	10 20 30 40 50 60 MKHKVSKRVI SLKWVPFLCI SFFALGAIFT SRSWEPSSDS GSQLISQHHR DHELQIVSDD 70 80 90 100 110 120 CAHNKKATQE KDVTGEVLRT HEAIQDDRSL DKSVSTLSST RSSQEMVDGS ETNPRKKVFM 130 140 150 160 170 180 VMGINTAFSS RKRRDSVRET WMPQGEKLER LEQEKGIVIK FMIGHSATSN SILDRAIDSE 190 200 210 220 230 240 DAQHKDFLRL EHVEGYHELS AKTKIFFSTA VAKWDAEFYI KVDDDVHVNL GMLASTLARH 250 260 270 280 290 300 RSKPRVYIGC MKSGPVLAQN LLNCFRTVKY HEPEYWKFGE DGNKYFRHAT GQIYAISKDL 310 320 330 340 350 360 ANYISINQPI LHKYANEDVS LGSWFIGLEV EHIDDRNFCC GTPPDCRWKA EAGDVCVASF 370 380 390 EWSCSGICKS VERMKIVHEV CSEGEGAVWN TLL
	Isoform 2. Checksum: 0DD6E4E6961E9412	FASTA	384	43,555
	10 20 30 40 50 60 MKHKVSKRVI SLKWVPFLCI SFFALGAIFT SRSWEPSSDS GSQLISQHHR DHELQIVSDD 70 80 90 100 110 120 CAHNKKATQE KDVTGEVLRT HEAIQSLDKS VSTLSSTRSS QEMVDGSETN PRKKVFMVMG 130 140 150 160 170 180 INTAFSSRKR RDSVRETWMP QGEKLERLEQ EKGIVIKFMI GHSATSNSIL DRAIDSEDAQ 190 200 210 220 230 240 HKDFLRLEHV EGYHELSAKT KIFFSTAVAK WDAEFYIKVD DDVHVNLGML ASTLARHRSK 250 260 270 280 290 300 PRVYIGCMKS GPVLAQKTVK YHEPEYWKFG EDGNKYFRHA TGQIYAISKD LANYISINQP 310 320 330 340 350 360 ILHKYANEDV SLGSWFIGLE VEHIDDRNFC CGTPPDCRWK AEAGDVCVAS FEWSCSGICK 370 380 SVERMKIVHE VCSEGEGAVW NTLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases." Qu Y., Egelund J., Gilson P.R., Houghton F., Gleeson P.A., Schultz C.J., Bacic A. Plant Mol. Biol. 68:43-59(2008) [PubMed: 18548197] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC009243 Genomic DNA. Translation: AAF17702.1. Sequence problems. AC012193 Genomic DNA. Translation: AAG51626.1. Sequence problems. BT010541 mRNA. Translation: AAQ65164.1. AK175483 mRNA. Translation: BAD43246.1. AK221383 mRNA. Translation: BAD94299.1.
IPI	IPI00519171. IPI00545730.
PIR	B96808.
RefSeq	NP_177904.3. NP_974164.2.
UniGene	At.28097
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Proteomic databases
PRIDE	Q6NQB7.
Genome annotation databases
GeneID	844443.
GenomeReviews	Gene locus AT1G77810 in contig CT485782_GR.
KEGG	ath:AT1G77810.
NMPDR	fig|3702.1.peg.7325.
Organism-specific databases
TAIR	At1g77810.
Phylogenomic databases
OMA	KAIDSED.
Gene expression databases
Genevestigator	Q6NQB7.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GT7_ARATH

Accession

Primary (citable) accession number: Q6NQB7
Secondary accession number(s): Q3ECB6, Q9CA13, Q9SH19

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)