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Protein

Probable apyrase 5

Gene

APY5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates.By similarity

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei196Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi73 – 83ATP-bindingCuratedAdd BLAST11
Nucleotide bindingi220 – 230ATP-bindingCuratedAdd BLAST11

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G14250-MONOMER.
ReactomeiR-ATH-8850843. Phosphate bond hydrolysis by NTPDase proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable apyrase 5 (EC:3.6.1.5)
Short name:
AtAPY5
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name:
ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 5
Gene namesi
Name:APY5
Ordered Locus Names:At1g14250
ORF Names:F14L17.1, F7A19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G14250.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 32CytoplasmicSequence analysisAdd BLAST32
Transmembranei33 – 53Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini54 – 488ExtracellularSequence analysisAdd BLAST435

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004203431 – 488Probable apyrase 5Add BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi251N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6NQA8.

PTM databases

iPTMnetiQ6NQA8.

Expressioni

Tissue specificityi

Highly expressed in young rosette leaves but only weakly in roots.1 Publication

Inductioni

By wounding and drought stress.1 Publication

Gene expression databases

GenevisibleiQ6NQA8. AT.

Interactioni

Protein-protein interaction databases

BioGridi23226. 1 interactor.
STRINGi3702.AT1G14250.1.

Structurei

3D structure databases

ProteinModelPortaliQ6NQA8.
SMRiQ6NQA8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1386. Eukaryota.
COG5371. LUCA.
HOGENOMiHOG000077482.
InParanoidiQ6NQA8.
KOiK01510.
OMAiGKENCAY.
OrthoDBiEOG09360BC0.
PhylomeDBiQ6NQA8.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NQA8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALKVQILP DNQSSPSSTH MLTKPKSKKA TKSIAMLIVA SLAITLGLLF
60 70 80 90 100
VFSSNSVMFS ASFLRRSSLH YSVIIDAGSS GTRIHVFGYW FESGKPVFDF
110 120 130 140 150
GEEHYASLKL SPGLSSYADN PEGASVSVTK LVEFAKGRIP KGKLKKSDIR
160 170 180 190 200
LMATAGMRLL DVPVQEQILD VTRRVLRSSG FKFQDEWATV ISGTDEGIYA
210 220 230 240 250
WVVANHALGS LGGDPLKTTG IVELGGASAQ VTFVPSEHVP PEFSRTISYG
260 270 280 290 300
NVSYTIYSHS FLDFGQDAAE DKLLESLQNS VAASTGDGIV EDPCTPKGYI
310 320 330 340 350
YDTHSQKDSS GFLSEESKFK ASLQVQAAGD FTKCRSATLA MLQEGKENCA
360 370 380 390 400
YKHCSIGSTF TPNIQGSFLA TENFFHTSKF FGLGEKEWLS EMILAGKRFC
410 420 430 440 450
GEEWSKLKEK YPTTKDKYLH RYCFSSAYII SMLHDSLGVA LDDERIKYAS
460 470 480
KAGKENIPLD WALGAFILNT DTPTSDYNGK SRKMIGFK
Length:488
Mass (Da):53,505
Last modified:July 5, 2004 - v1
Checksum:i75D5ED11E61F786A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JF830010 mRNA. Translation: AEJ38086.1.
AC007576 Genomic DNA. No translation available.
AC012188 Genomic DNA. Translation: AAF43924.1.
CP002684 Genomic DNA. Translation: AEE29131.1.
BT010550 mRNA. Translation: AAQ65173.1.
AK175135 mRNA. Translation: BAD42898.1.
AK175519 mRNA. Translation: BAD43282.1.
PIRiE86276.
RefSeqiNP_172877.1. NM_101291.3.
UniGeneiAt.41981.

Genome annotation databases

EnsemblPlantsiAT1G14250.1; AT1G14250.1; AT1G14250.
GeneIDi837986.
GrameneiAT1G14250.1; AT1G14250.1; AT1G14250.
KEGGiath:AT1G14250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JF830010 mRNA. Translation: AEJ38086.1.
AC007576 Genomic DNA. No translation available.
AC012188 Genomic DNA. Translation: AAF43924.1.
CP002684 Genomic DNA. Translation: AEE29131.1.
BT010550 mRNA. Translation: AAQ65173.1.
AK175135 mRNA. Translation: BAD42898.1.
AK175519 mRNA. Translation: BAD43282.1.
PIRiE86276.
RefSeqiNP_172877.1. NM_101291.3.
UniGeneiAt.41981.

3D structure databases

ProteinModelPortaliQ6NQA8.
SMRiQ6NQA8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23226. 1 interactor.
STRINGi3702.AT1G14250.1.

PTM databases

iPTMnetiQ6NQA8.

Proteomic databases

PaxDbiQ6NQA8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G14250.1; AT1G14250.1; AT1G14250.
GeneIDi837986.
GrameneiAT1G14250.1; AT1G14250.1; AT1G14250.
KEGGiath:AT1G14250.

Organism-specific databases

TAIRiAT1G14250.

Phylogenomic databases

eggNOGiKOG1386. Eukaryota.
COG5371. LUCA.
HOGENOMiHOG000077482.
InParanoidiQ6NQA8.
KOiK01510.
OMAiGKENCAY.
OrthoDBiEOG09360BC0.
PhylomeDBiQ6NQA8.

Enzyme and pathway databases

BioCyciARA:AT1G14250-MONOMER.
ReactomeiR-ATH-8850843. Phosphate bond hydrolysis by NTPDase proteins.

Miscellaneous databases

PROiQ6NQA8.

Gene expression databases

GenevisibleiQ6NQA8. AT.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPY5_ARATH
AccessioniPrimary (citable) accession number: Q6NQA8
Secondary accession number(s): Q9M9T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.