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Q6NQA8 (APY5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable apyrase 5

Short name=AtAPY5
EC=3.6.1.5
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name=ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 5
Gene names
Name:APY5
Ordered Locus Names:At1g14250
ORF Names:F14L17.1, F7A19
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length488 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates By similarity.

Catalytic activity

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactor

Calcium By similarity.

Subcellular location

Membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

Highly expressed in young rosette leaves but only weakly in roots. Ref.1

Induction

By wounding and drought stress. Ref.1

Disruption phenotype

No visible phenotype. Ref.1

Sequence similarities

Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
   Cellular componentMembrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   LigandATP-binding
Calcium
Nucleotide-binding
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

vacuole

Inferred from direct assay PubMed 15539469. Source: TAIR

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 488488Probable apyrase 5
PRO_0000420343

Regions

Topological domain1 – 3232Cytoplasmic Potential
Transmembrane33 – 5321Helical; Signal-anchor for type II membrane protein; Potential
Topological domain54 – 488435Extracellular Potential
Nucleotide binding73 – 8311ATP-binding Probable
Nucleotide binding220 – 23011ATP-binding Probable

Sites

Active site1961Proton acceptor By similarity

Amino acid modifications

Glycosylation2511N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q6NQA8 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 75D5ED11E61F786A

FASTA48853,505
        10         20         30         40         50         60 
MDALKVQILP DNQSSPSSTH MLTKPKSKKA TKSIAMLIVA SLAITLGLLF VFSSNSVMFS 

        70         80         90        100        110        120 
ASFLRRSSLH YSVIIDAGSS GTRIHVFGYW FESGKPVFDF GEEHYASLKL SPGLSSYADN 

       130        140        150        160        170        180 
PEGASVSVTK LVEFAKGRIP KGKLKKSDIR LMATAGMRLL DVPVQEQILD VTRRVLRSSG 

       190        200        210        220        230        240 
FKFQDEWATV ISGTDEGIYA WVVANHALGS LGGDPLKTTG IVELGGASAQ VTFVPSEHVP 

       250        260        270        280        290        300 
PEFSRTISYG NVSYTIYSHS FLDFGQDAAE DKLLESLQNS VAASTGDGIV EDPCTPKGYI 

       310        320        330        340        350        360 
YDTHSQKDSS GFLSEESKFK ASLQVQAAGD FTKCRSATLA MLQEGKENCA YKHCSIGSTF 

       370        380        390        400        410        420 
TPNIQGSFLA TENFFHTSKF FGLGEKEWLS EMILAGKRFC GEEWSKLKEK YPTTKDKYLH 

       430        440        450        460        470        480 
RYCFSSAYII SMLHDSLGVA LDDERIKYAS KAGKENIPLD WALGAFILNT DTPTSDYNGK 


SRKMIGFK 

« Hide

References

« Hide 'large scale' references
[1]"Functional analyses of Arabidopsis apyrases 3 through 7."
Yang J.
Thesis (2011), University of Texas, United States
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
JF830010 mRNA. Translation: AEJ38086.1.
AC007576 Genomic DNA. No translation available.
AC012188 Genomic DNA. Translation: AAF43924.1.
CP002684 Genomic DNA. Translation: AEE29131.1.
BT010550 mRNA. Translation: AAQ65173.1.
AK175135 mRNA. Translation: BAD42898.1.
AK175519 mRNA. Translation: BAD43282.1.
PIRE86276.
RefSeqNP_172877.1. NM_101291.2.
UniGeneAt.41981.

3D structure databases

ProteinModelPortalQ6NQA8.
SMRQ6NQA8. Positions 68-479.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G14250.1-P.

Proteomic databases

PaxDbQ6NQA8.
PRIDEQ6NQA8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G14250.1; AT1G14250.1; AT1G14250.
GeneID837986.
KEGGath:AT1G14250.

Organism-specific databases

GeneFarm3050. 285.
TAIRAT1G14250.

Phylogenomic databases

eggNOGCOG5371.
HOGENOMHOG000077482.
InParanoidQ6NQA8.
KOK01510.
OMAGKENCAY.
PhylomeDBQ6NQA8.

Enzyme and pathway databases

BioCycARA:AT1G14250-MONOMER.

Gene expression databases

GenevestigatorQ6NQA8.

Family and domain databases

InterProIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERPTHR11782. PTHR11782. 1 hit.
PfamPF01150. GDA1_CD39. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPY5_ARATH
AccessionPrimary (citable) accession number: Q6NQA8
Secondary accession number(s): Q9M9T7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names