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Q6NQA8

- APY5_ARATH

UniProt

Q6NQA8 - APY5_ARATH

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Protein
Probable apyrase 5
Gene
APY5, At1g14250, F14L17.1, F7A19
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates By similarity.

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Calcium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei196 – 1961Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 8311ATP-binding Inferred
Add
BLAST
Nucleotide bindingi220 – 23011ATP-binding Inferred
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Calcium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G14250-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable apyrase 5 (EC:3.6.1.5)
    Short name:
    AtAPY5
    Alternative name(s):
    ATP-diphosphatase
    ATP-diphosphohydrolase
    Adenosine diphosphatase
    Short name:
    ADPase
    NTPDase
    Nucleoside triphosphate diphosphohydrolase 5
    Gene namesi
    Name:APY5
    Ordered Locus Names:At1g14250
    ORF Names:F14L17.1, F7A19
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G14250.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3232Cytoplasmic Reviewed prediction
    Add
    BLAST
    Transmembranei33 – 5321Helical; Signal-anchor for type II membrane protein; Reviewed prediction
    Add
    BLAST
    Topological domaini54 – 488435Extracellular Reviewed prediction
    Add
    BLAST

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. vacuole Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 488488Probable apyrase 5
    PRO_0000420343Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi251 – 2511N-linked (GlcNAc...) Reviewed prediction

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ6NQA8.
    PRIDEiQ6NQA8.

    Expressioni

    Tissue specificityi

    Highly expressed in young rosette leaves but only weakly in roots.1 Publication

    Inductioni

    By wounding and drought stress.1 Publication

    Gene expression databases

    GenevestigatoriQ6NQA8.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G14250.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6NQA8.
    SMRiQ6NQA8. Positions 68-479.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GDA1/CD39 NTPase family.

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5371.
    HOGENOMiHOG000077482.
    InParanoidiQ6NQA8.
    KOiK01510.
    OMAiGKENCAY.
    PhylomeDBiQ6NQA8.

    Family and domain databases

    InterProiIPR000407. GDA1_CD39_NTPase.
    [Graphical view]
    PANTHERiPTHR11782. PTHR11782. 1 hit.
    PfamiPF01150. GDA1_CD39. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6NQA8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDALKVQILP DNQSSPSSTH MLTKPKSKKA TKSIAMLIVA SLAITLGLLF    50
    VFSSNSVMFS ASFLRRSSLH YSVIIDAGSS GTRIHVFGYW FESGKPVFDF 100
    GEEHYASLKL SPGLSSYADN PEGASVSVTK LVEFAKGRIP KGKLKKSDIR 150
    LMATAGMRLL DVPVQEQILD VTRRVLRSSG FKFQDEWATV ISGTDEGIYA 200
    WVVANHALGS LGGDPLKTTG IVELGGASAQ VTFVPSEHVP PEFSRTISYG 250
    NVSYTIYSHS FLDFGQDAAE DKLLESLQNS VAASTGDGIV EDPCTPKGYI 300
    YDTHSQKDSS GFLSEESKFK ASLQVQAAGD FTKCRSATLA MLQEGKENCA 350
    YKHCSIGSTF TPNIQGSFLA TENFFHTSKF FGLGEKEWLS EMILAGKRFC 400
    GEEWSKLKEK YPTTKDKYLH RYCFSSAYII SMLHDSLGVA LDDERIKYAS 450
    KAGKENIPLD WALGAFILNT DTPTSDYNGK SRKMIGFK 488
    Length:488
    Mass (Da):53,505
    Last modified:July 5, 2004 - v1
    Checksum:i75D5ED11E61F786A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JF830010 mRNA. Translation: AEJ38086.1.
    AC007576 Genomic DNA. No translation available.
    AC012188 Genomic DNA. Translation: AAF43924.1.
    CP002684 Genomic DNA. Translation: AEE29131.1.
    BT010550 mRNA. Translation: AAQ65173.1.
    AK175135 mRNA. Translation: BAD42898.1.
    AK175519 mRNA. Translation: BAD43282.1.
    PIRiE86276.
    RefSeqiNP_172877.1. NM_101291.2.
    UniGeneiAt.41981.

    Genome annotation databases

    EnsemblPlantsiAT1G14250.1; AT1G14250.1; AT1G14250.
    GeneIDi837986.
    KEGGiath:AT1G14250.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JF830010 mRNA. Translation: AEJ38086.1 .
    AC007576 Genomic DNA. No translation available.
    AC012188 Genomic DNA. Translation: AAF43924.1 .
    CP002684 Genomic DNA. Translation: AEE29131.1 .
    BT010550 mRNA. Translation: AAQ65173.1 .
    AK175135 mRNA. Translation: BAD42898.1 .
    AK175519 mRNA. Translation: BAD43282.1 .
    PIRi E86276.
    RefSeqi NP_172877.1. NM_101291.2.
    UniGenei At.41981.

    3D structure databases

    ProteinModelPortali Q6NQA8.
    SMRi Q6NQA8. Positions 68-479.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G14250.1-P.

    Proteomic databases

    PaxDbi Q6NQA8.
    PRIDEi Q6NQA8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G14250.1 ; AT1G14250.1 ; AT1G14250 .
    GeneIDi 837986.
    KEGGi ath:AT1G14250.

    Organism-specific databases

    GeneFarmi 3050. 285.
    TAIRi AT1G14250.

    Phylogenomic databases

    eggNOGi COG5371.
    HOGENOMi HOG000077482.
    InParanoidi Q6NQA8.
    KOi K01510.
    OMAi GKENCAY.
    PhylomeDBi Q6NQA8.

    Enzyme and pathway databases

    BioCyci ARA:AT1G14250-MONOMER.

    Gene expression databases

    Genevestigatori Q6NQA8.

    Family and domain databases

    InterProi IPR000407. GDA1_CD39_NTPase.
    [Graphical view ]
    PANTHERi PTHR11782. PTHR11782. 1 hit.
    Pfami PF01150. GDA1_CD39. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Functional analyses of Arabidopsis apyrases 3 through 7."
      Yang J.
      Thesis (2011), University of Texas, United States
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiAPY5_ARATH
    AccessioniPrimary (citable) accession number: Q6NQA8
    Secondary accession number(s): Q9M9T7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: July 5, 2004
    Last modified: June 11, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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