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Protein

Probable apyrase 5

Gene

APY5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates.By similarity

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei196Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi73 – 83ATP-bindingCuratedAdd BLAST11
Nucleotide bindingi220 – 230ATP-bindingCuratedAdd BLAST11

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
LigandATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G14250-MONOMER
ReactomeiR-ATH-8850843 Phosphate bond hydrolysis by NTPDase proteins

Names & Taxonomyi

Protein namesi
Recommended name:
Probable apyrase 5 (EC:3.6.1.5)
Short name:
AtAPY5
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name:
ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 5
Gene namesi
Name:APY5
Ordered Locus Names:At1g14250
ORF Names:F14L17.1, F7A19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G14250
TAIRilocus:2035802 AT1G14250

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 32CytoplasmicSequence analysisAdd BLAST32
Transmembranei33 – 53Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini54 – 488ExtracellularSequence analysisAdd BLAST435

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004203431 – 488Probable apyrase 5Add BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi251N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6NQA8

PTM databases

iPTMnetiQ6NQA8

Expressioni

Tissue specificityi

Highly expressed in young rosette leaves but only weakly in roots.1 Publication

Inductioni

By wounding and drought stress.1 Publication

Gene expression databases

ExpressionAtlasiQ6NQA8 baseline and differential
GenevisibleiQ6NQA8 AT

Interactioni

Protein-protein interaction databases

BioGridi23226, 1 interactor
STRINGi3702.AT1G14250.1

Structurei

3D structure databases

ProteinModelPortaliQ6NQA8
SMRiQ6NQA8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1386 Eukaryota
COG5371 LUCA
HOGENOMiHOG000077482
InParanoidiQ6NQA8
KOiK01510
OMAiSAWMYQV
OrthoDBiEOG09360BC0
PhylomeDBiQ6NQA8

Family and domain databases

InterProiView protein in InterPro
IPR000407 GDA1_CD39_NTPase
PANTHERiPTHR11782 PTHR11782, 1 hit
PfamiView protein in Pfam
PF01150 GDA1_CD39, 1 hit

Sequencei

Sequence statusi: Complete.

Q6NQA8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALKVQILP DNQSSPSSTH MLTKPKSKKA TKSIAMLIVA SLAITLGLLF
60 70 80 90 100
VFSSNSVMFS ASFLRRSSLH YSVIIDAGSS GTRIHVFGYW FESGKPVFDF
110 120 130 140 150
GEEHYASLKL SPGLSSYADN PEGASVSVTK LVEFAKGRIP KGKLKKSDIR
160 170 180 190 200
LMATAGMRLL DVPVQEQILD VTRRVLRSSG FKFQDEWATV ISGTDEGIYA
210 220 230 240 250
WVVANHALGS LGGDPLKTTG IVELGGASAQ VTFVPSEHVP PEFSRTISYG
260 270 280 290 300
NVSYTIYSHS FLDFGQDAAE DKLLESLQNS VAASTGDGIV EDPCTPKGYI
310 320 330 340 350
YDTHSQKDSS GFLSEESKFK ASLQVQAAGD FTKCRSATLA MLQEGKENCA
360 370 380 390 400
YKHCSIGSTF TPNIQGSFLA TENFFHTSKF FGLGEKEWLS EMILAGKRFC
410 420 430 440 450
GEEWSKLKEK YPTTKDKYLH RYCFSSAYII SMLHDSLGVA LDDERIKYAS
460 470 480
KAGKENIPLD WALGAFILNT DTPTSDYNGK SRKMIGFK
Length:488
Mass (Da):53,505
Last modified:July 5, 2004 - v1
Checksum:i75D5ED11E61F786A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JF830010 mRNA Translation: AEJ38086.1
AC007576 Genomic DNA No translation available.
AC012188 Genomic DNA Translation: AAF43924.1
CP002684 Genomic DNA Translation: AEE29131.1
BT010550 mRNA Translation: AAQ65173.1
AK175135 mRNA Translation: BAD42898.1
AK175519 mRNA Translation: BAD43282.1
PIRiE86276
RefSeqiNP_172877.1, NM_101291.3
UniGeneiAt.41981

Genome annotation databases

EnsemblPlantsiAT1G14250.1; AT1G14250.1; AT1G14250
GeneIDi837986
GrameneiAT1G14250.1; AT1G14250.1; AT1G14250
KEGGiath:AT1G14250

Similar proteinsi

Entry informationi

Entry nameiAPY5_ARATH
AccessioniPrimary (citable) accession number: Q6NQA8
Secondary accession number(s): Q9M9T7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: July 5, 2004
Last modified: April 25, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health