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Protein

NADPH-dependent diflavin oxidoreductase 1

Gene

ATR3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Transfers electrons from NADPH to the Fe-S cluster of the anamorsin/DRE2 homolog (By similarity). Catalyzes the NADP-dependent reduction of cytochrome c, but not cytochrome P450 in vitro. Required for embryo development.UniRule annotation1 Publication

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotation
  • FMNUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei135FMNUniRule annotation1
Binding sitei369FADUniRule annotation1
Binding sitei475NADPUniRule annotation1
Binding sitei583NADPUniRule annotation1
Binding sitei622FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 19FMNUniRule annotation6
Nucleotide bindingi62 – 65FMNUniRule annotation4
Nucleotide bindingi399 – 402FADUniRule annotation4
Nucleotide bindingi433 – 436FADUniRule annotation4
Nucleotide bindingi541 – 542NADPUniRule annotation2
Nucleotide bindingi547 – 551NADPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciARA:AT3G02280-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent diflavin oxidoreductase 1UniRule annotation (EC:1.18.1.-UniRule annotation)
Alternative name(s):
NADPH-dependent FMN and FAD-containing oxidoreductaseUniRule annotation
Gene namesi
Name:ATR3
Ordered Locus Names:At3g02280
ORF Names:F14P3.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G02280
TAIRilocus:2076547 AT3G02280

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality when homozygous. Embryo development arrested at 2 to 4-cell stages.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004168411 – 623NADPH-dependent diflavin oxidoreductase 1Add BLAST623

Proteomic databases

PaxDbiQ6NPS8
PRIDEiQ6NPS8

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Developmental stagei

Expressed in cell cycle-dependent manner. Most abundant at the G2 and G2/M transition. Lowest expression in S and M phases.1 Publication

Gene expression databases

ExpressionAtlasiQ6NPS8 baseline and differential
GenevisibleiQ6NPS8 AT

Interactioni

Subunit structurei

Interacts with At5g18400.1 Publication

Protein-protein interaction databases

BioGridi5751, 1 interactor
STRINGi3702.AT3G02280.1

Structurei

3D structure databases

ProteinModelPortaliQ6NPS8
SMRiQ6NPS8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 153Flavodoxin-likeUniRule annotationAdd BLAST146
Domaini221 – 467FAD-binding FR-typeUniRule annotationAdd BLAST247

Sequence similaritiesi

Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1159 Eukaryota
COG0369 LUCA
HOGENOMiHOG000173033
InParanoidiQ6NPS8
OMAiGPSHFQD
OrthoDBiEOG093604Q6
PhylomeDBiQ6NPS8

Family and domain databases

Gene3Di1.20.990.10, 1 hit
3.40.50.360, 1 hit
HAMAPiMF_03178 NDOR1, 1 hit
InterProiView protein in InterPro
IPR003097 FAD-binding_1
IPR017927 Fd_Rdtase_FAD-bd
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR028879 NDOR1
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00667 FAD_binding_1, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SUPFAMiSSF52218 SSF52218, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Q6NPS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEKQRKLLV LYASQTGNAL DAAERIGREA ERRGLPASVV STDEFDTSSL
60 70 80 90 100
PHHEEAVVFV VSTTGQGDSP DSFKAFWRFL LQRNLGNYWL QQVRYAVFGL
110 120 130 140 150
GDSGYQKYNF VAKKLDKRLS DLGATTIIEK GLGDDQHPSG YEGTLDPWML
160 170 180 190 200
SLWRTLYQIN PKYFPKGPDV KIPQDEVIDK PKYRILFHKQ EKLEPKLLSD
210 220 230 240 250
SDIIQRARGM SPGKLFKDKS KPDCFLKMTR NEVLTKAEST KDVRHFEFQF
260 270 280 290 300
VSSTIEYEVG DVVELLPSQN SSVVDAFIER CGLDPESFIT VGPRETENSS
310 320 330 340 350
FSEEMITQIP IKLKTFVELT MDVTSASPRR YFFEIMSFYA TAEHEKERLQ
360 370 380 390 400
YFASPEGRDD LYNYNQKERR SILEVLEDFP SVQIPFDWLV QLVPPLKPRA
410 420 430 440 450
FSISSSPLAH PAAVHLTVSI VSWITPYKRT RKGLCSSWLA SLAPEQEVNI
460 470 480 490 500
PVWFHKGSLP APSQSLPLIL VGPGTGCAPF RGFIAERAVQ AQSSPVAPVM
510 520 530 540 550
FFFGCRNKDT DFLYRDFWES HAREGGMLSE GKGGGFYTAF SRDQPKKVYV
560 570 580 590 600
QHKIREMSKR VWDLLCDGAA VYVAGSSTKM PCDVMSAFED IVSEETGGGS
610 620
KEVASRWLKA LEKTGRYNVE AWS
Length:623
Mass (Da):70,412
Last modified:July 5, 2004 - v1
Checksum:i6778218FB5004A95
GO

Sequence cautioni

The sequence AAF02110 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009755 Genomic DNA Translation: AAF02110.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE73787.1
CP002686 Genomic DNA Translation: ANM65261.1
CP002686 Genomic DNA Translation: ANM65262.1
BT010739 mRNA Translation: AAR23709.1
AK229452 mRNA Translation: BAF01311.1
RefSeqiNP_001327241.1, NM_001337391.1
NP_001327242.1, NM_001337392.1
NP_186877.2, NM_111095.5
UniGeneiAt.27987

Genome annotation databases

EnsemblPlantsiAT3G02280.1; AT3G02280.1; AT3G02280
AT3G02280.2; AT3G02280.2; AT3G02280
AT3G02280.3; AT3G02280.3; AT3G02280
GeneIDi820417
GrameneiAT3G02280.1; AT3G02280.1; AT3G02280
AT3G02280.2; AT3G02280.2; AT3G02280
AT3G02280.3; AT3G02280.3; AT3G02280
KEGGiath:AT3G02280

Similar proteinsi

Entry informationi

Entry nameiNDOR1_ARATH
AccessioniPrimary (citable) accession number: Q6NPS8
Secondary accession number(s): A0A1I9LRQ3, Q9SRU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: July 5, 2004
Last modified: April 25, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health