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Protein

NADPH-dependent diflavin oxidoreductase 1

Gene

ATR3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Transfers electrons from NADPH to the Fe-S cluster of the anamorsin/DRE2 homolog (By similarity). Catalyzes the NADP-dependent reduction of cytochrome c, but not cytochrome P450 in vitro. Required for embryo development.UniRule annotation1 Publication

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotation
  • FMNUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei135FMNUniRule annotation1
Binding sitei369FADUniRule annotation1
Binding sitei475NADPUniRule annotation1
Binding sitei583NADPUniRule annotation1
Binding sitei622FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 19FMNUniRule annotation6
Nucleotide bindingi62 – 65FMNUniRule annotation4
Nucleotide bindingi399 – 402FADUniRule annotation4
Nucleotide bindingi433 – 436FADUniRule annotation4
Nucleotide bindingi541 – 542NADPUniRule annotation2
Nucleotide bindingi547 – 551NADPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: UniProtKB
  • iron-sulfur cluster assembly Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciARA:AT3G02280-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent diflavin oxidoreductase 1UniRule annotation (EC:1.18.1.-UniRule annotation)
Alternative name(s):
NADPH-dependent FMN and FAD-containing oxidoreductaseUniRule annotation
Gene namesi
Name:ATR3
Ordered Locus Names:At3g02280
ORF Names:F14P3.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G02280.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality when homozygous. Embryo development arrested at 2 to 4-cell stages.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004168411 – 623NADPH-dependent diflavin oxidoreductase 1Add BLAST623

Proteomic databases

PaxDbiQ6NPS8.
PRIDEiQ6NPS8.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Developmental stagei

Expressed in cell cycle-dependent manner. Most abundant at the G2 and G2/M transition. Lowest expression in S and M phases.1 Publication

Gene expression databases

GenevisibleiQ6NPS8. AT.

Interactioni

Subunit structurei

Interacts with At5g18400.1 Publication

Protein-protein interaction databases

BioGridi5751. 1 interactor.
STRINGi3702.AT3G02280.1.

Structurei

3D structure databases

ProteinModelPortaliQ6NPS8.
SMRiQ6NPS8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 153Flavodoxin-likeUniRule annotationAdd BLAST146
Domaini221 – 467FAD-binding FR-typeUniRule annotationAdd BLAST247

Sequence similaritiesi

Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG1159. Eukaryota.
COG0369. LUCA.
HOGENOMiHOG000173033.
InParanoidiQ6NPS8.
OMAiAIPPDYL.
OrthoDBiEOG093604Q6.
PhylomeDBiQ6NPS8.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03178. NDOR1. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR028879. NDOR1.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NPS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEKQRKLLV LYASQTGNAL DAAERIGREA ERRGLPASVV STDEFDTSSL
60 70 80 90 100
PHHEEAVVFV VSTTGQGDSP DSFKAFWRFL LQRNLGNYWL QQVRYAVFGL
110 120 130 140 150
GDSGYQKYNF VAKKLDKRLS DLGATTIIEK GLGDDQHPSG YEGTLDPWML
160 170 180 190 200
SLWRTLYQIN PKYFPKGPDV KIPQDEVIDK PKYRILFHKQ EKLEPKLLSD
210 220 230 240 250
SDIIQRARGM SPGKLFKDKS KPDCFLKMTR NEVLTKAEST KDVRHFEFQF
260 270 280 290 300
VSSTIEYEVG DVVELLPSQN SSVVDAFIER CGLDPESFIT VGPRETENSS
310 320 330 340 350
FSEEMITQIP IKLKTFVELT MDVTSASPRR YFFEIMSFYA TAEHEKERLQ
360 370 380 390 400
YFASPEGRDD LYNYNQKERR SILEVLEDFP SVQIPFDWLV QLVPPLKPRA
410 420 430 440 450
FSISSSPLAH PAAVHLTVSI VSWITPYKRT RKGLCSSWLA SLAPEQEVNI
460 470 480 490 500
PVWFHKGSLP APSQSLPLIL VGPGTGCAPF RGFIAERAVQ AQSSPVAPVM
510 520 530 540 550
FFFGCRNKDT DFLYRDFWES HAREGGMLSE GKGGGFYTAF SRDQPKKVYV
560 570 580 590 600
QHKIREMSKR VWDLLCDGAA VYVAGSSTKM PCDVMSAFED IVSEETGGGS
610 620
KEVASRWLKA LEKTGRYNVE AWS
Length:623
Mass (Da):70,412
Last modified:July 5, 2004 - v1
Checksum:i6778218FB5004A95
GO

Sequence cautioni

The sequence AAF02110 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009755 Genomic DNA. Translation: AAF02110.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE73787.1.
BT010739 mRNA. Translation: AAR23709.1.
AK229452 mRNA. Translation: BAF01311.1.
RefSeqiNP_186877.2. NM_111095.5.
UniGeneiAt.27987.

Genome annotation databases

EnsemblPlantsiAT3G02280.1; AT3G02280.1; AT3G02280.
GeneIDi820417.
GrameneiAT3G02280.1; AT3G02280.1; AT3G02280.
KEGGiath:AT3G02280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009755 Genomic DNA. Translation: AAF02110.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE73787.1.
BT010739 mRNA. Translation: AAR23709.1.
AK229452 mRNA. Translation: BAF01311.1.
RefSeqiNP_186877.2. NM_111095.5.
UniGeneiAt.27987.

3D structure databases

ProteinModelPortaliQ6NPS8.
SMRiQ6NPS8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5751. 1 interactor.
STRINGi3702.AT3G02280.1.

Proteomic databases

PaxDbiQ6NPS8.
PRIDEiQ6NPS8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G02280.1; AT3G02280.1; AT3G02280.
GeneIDi820417.
GrameneiAT3G02280.1; AT3G02280.1; AT3G02280.
KEGGiath:AT3G02280.

Organism-specific databases

TAIRiAT3G02280.

Phylogenomic databases

eggNOGiKOG1159. Eukaryota.
COG0369. LUCA.
HOGENOMiHOG000173033.
InParanoidiQ6NPS8.
OMAiAIPPDYL.
OrthoDBiEOG093604Q6.
PhylomeDBiQ6NPS8.

Enzyme and pathway databases

BioCyciARA:AT3G02280-MONOMER.

Miscellaneous databases

PROiQ6NPS8.

Gene expression databases

GenevisibleiQ6NPS8. AT.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03178. NDOR1. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR028879. NDOR1.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDOR1_ARATH
AccessioniPrimary (citable) accession number: Q6NPS8
Secondary accession number(s): Q9SRU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.