Q6NMM8 (F8H_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glucuronoxylan glucuronosyltransferase F8H
EC=2.4.1.-

Alternative name(s):
FRA8 homolog
Protein FRAGILE FIBER 8 homolog

Gene names

Name:	F8H
Ordered Locus Names:	At5g22940
ORF Names:	MRN17.17

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the synthesis of the hemicellulose glucuronoxylan, a major component of secondary cell walls. Probably involved in the synthesis of the glycosyl sequence at the glucuronoxylan reducing end. Ref.5
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable Ref.5.
Tissue specificity	Expressed in xylem cells in stems and in roots. Ref.5
Disruption phenotype	No visible phenotype. Ref.5
Sequence similarities	Belongs to the glycosyltransferase 47 family.
Sequence caution	The sequence BAB10615.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW glucuronoxylan biosynthetic process Inferred from genetic interaction Ref.5. Source: TAIR
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Probable glucuronoxylan glucuronosyltransferase F8H		PRO_0000407575
Regions
	Topological domain	1 – 36	36	Cytoplasmic Potential
	Transmembrane	37 – 57	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	58 – 469	412	Lumenal Potential
Amino acid modifications
	Glycosylation	171	1	N-linked (GlcNAc...) Potential
	Glycosylation	203	1	N-linked (GlcNAc...) Potential
	Glycosylation	301	1	N-linked (GlcNAc...) Potential
	Glycosylation	411	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6NMM8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 13B860CA19D1F729	FASTA	469	53,809
	10 20 30 40 50 60 MSLDIKKPNI TKTKKKKTGF VVKMQLNNNR GGNKRNIFIF FFFRNYYTWI LWFCLSLYFF 70 80 90 100 110 120 TSYFSVEDQS SPSSIRLLSN HKTSSSLPSR ALIESSAIKT TSIGLFTGMK IYVYDLPASY 130 140 150 160 170 180 NDDWVTASDR CASHLFAAEV AIHRALLSSD VRTLDPDEAD YFFVPVYVSC NFSTSNGFPS 190 200 210 220 230 240 LSHARSLLSS AVDFLSDHYP FWNRSQGSDH VFVASHDFGA CFHAMEDMAI EEGIPKFMKR 250 260 270 280 290 300 SIILQTFGVK YKHPCQEVEH VVIPPYIPPE SVQKAIEKAP VNGRRDIWAF FRGKMEVNPK 310 320 330 340 350 360 NISGRFYSKG VRTAILKKFG GRRRFYLNRH RFAGYRSEIV RSVFCLCPLG WAPWSPRLVE 370 380 390 400 410 420 SAVLGCVPVV IADGIQLPFS ETVQWPEISL TVAEKDVRNL RKVLEHVAAT NLSAIQRNLH 430 440 450 460 EPVFKRALLY NVPMKEGDAT WHILESLWRK LDDRSYRRSR VLSQREVDM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The F8H glycosyltransferase is a functional paralog of FRA8 involved in glucuronoxylan biosynthesis in Arabidopsis." Lee C., Teng Q., Huang W., Zhong R., Ye Z.H. Plant Cell Physiol. 50:812-827(2009) [PubMed: 19224953] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB005243 Genomic DNA. Translation: BAB10615.1. Sequence problems. CP002688 Genomic DNA. Translation: AED93099.1. BT011629 mRNA. Translation: AAS47635.1. BT014962 mRNA. Translation: AAT47813.1.
IPI	IPI00539801.
RefSeq	NP_197685.2. NM_122200.3.
UniGene	At.44147.
3D structure databases
ProteinModelPortal	Q6NMM8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6NMM8.
Protein family/group databases
CAZy	GT47. Glycosyltransferase Family 47.
Proteomic databases
PRIDE	Q6NMM8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G22940.1; AT5G22940.1; AT5G22940.
GeneID	832358.
GenomeReviews	Gene locus AT5G22940 in contig BA000015_GR.
KEGG	ath:AT5G22940.
NMPDR	fig|3702.1.peg.24462.
Organism-specific databases
TAIR	At5g22940.
Phylogenomic databases
eggNOG	KOG1021.
GeneTree	EPGT00070000030424.
HOGENOM	HBG319192.
InParanoid	Q6NMM8.
OMA	ASHDFGA.
PhylomeDB	Q6NMM8.
ProtClustDB	CLSN2681061.
Gene expression databases
Genevestigator	Q6NMM8.
Family and domain databases
InterPro	IPR004263. Exostosin. [Graphical view]
Pfam	PF03016. Exostosin. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F8H_ARATH

Accession

Primary (citable) accession number: Q6NMM8
Secondary accession number(s): Q9FFB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 3, 2011
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program