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Q6NMM8 (F8H_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable glucuronoxylan glucuronosyltransferase F8H

EC=2.4.1.-
Alternative name(s):
FRA8 homolog
Protein FRAGILE FIBER 8 homolog
Gene names
Name:F8H
Ordered Locus Names:At5g22940
ORF Names:MRN17.17
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the synthesis of the hemicellulose glucuronoxylan, a major component of secondary cell walls. Probably involved in the synthesis of the glycosyl sequence at the glucuronoxylan reducing end. Ref.5

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein Probable Ref.5.

Tissue specificity

Expressed in xylem cells in stems and in roots. Ref.5

Disruption phenotype

No visible phenotype. Ref.5

Sequence similarities

Belongs to the glycosyltransferase 47 family.

Sequence caution

The sequence BAB10615.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 469469Probable glucuronoxylan glucuronosyltransferase F8H
PRO_0000407575

Regions

Topological domain1 – 3636Cytoplasmic Potential
Transmembrane37 – 5721Helical; Signal-anchor for type II membrane protein; Potential
Topological domain58 – 469412Lumenal Potential

Amino acid modifications

Glycosylation1711N-linked (GlcNAc...) Potential
Glycosylation2031N-linked (GlcNAc...) Potential
Glycosylation3011N-linked (GlcNAc...) Potential
Glycosylation4111N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q6NMM8 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 13B860CA19D1F729

FASTA46953,809
        10         20         30         40         50         60 
MSLDIKKPNI TKTKKKKTGF VVKMQLNNNR GGNKRNIFIF FFFRNYYTWI LWFCLSLYFF 

        70         80         90        100        110        120 
TSYFSVEDQS SPSSIRLLSN HKTSSSLPSR ALIESSAIKT TSIGLFTGMK IYVYDLPASY 

       130        140        150        160        170        180 
NDDWVTASDR CASHLFAAEV AIHRALLSSD VRTLDPDEAD YFFVPVYVSC NFSTSNGFPS 

       190        200        210        220        230        240 
LSHARSLLSS AVDFLSDHYP FWNRSQGSDH VFVASHDFGA CFHAMEDMAI EEGIPKFMKR 

       250        260        270        280        290        300 
SIILQTFGVK YKHPCQEVEH VVIPPYIPPE SVQKAIEKAP VNGRRDIWAF FRGKMEVNPK 

       310        320        330        340        350        360 
NISGRFYSKG VRTAILKKFG GRRRFYLNRH RFAGYRSEIV RSVFCLCPLG WAPWSPRLVE 

       370        380        390        400        410        420 
SAVLGCVPVV IADGIQLPFS ETVQWPEISL TVAEKDVRNL RKVLEHVAAT NLSAIQRNLH 

       430        440        450        460 
EPVFKRALLY NVPMKEGDAT WHILESLWRK LDDRSYRRSR VLSQREVDM 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Arabidopsis ORF clones."
Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"The F8H glycosyltransferase is a functional paralog of FRA8 involved in glucuronoxylan biosynthesis in Arabidopsis."
Lee C., Teng Q., Huang W., Zhong R., Ye Z.H.
Plant Cell Physiol. 50:812-827(2009) [PubMed: 19224953] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB005243 Genomic DNA. Translation: BAB10615.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED93099.1.
BT011629 mRNA. Translation: AAS47635.1.
BT014962 mRNA. Translation: AAT47813.1.
IPIIPI00539801.
RefSeqNP_197685.2. NM_122200.3.
UniGeneAt.44147.

3D structure databases

ProteinModelPortalQ6NMM8.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ6NMM8.

Protein family/group databases

CAZyGT47. Glycosyltransferase Family 47.

Proteomic databases

PRIDEQ6NMM8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G22940.1; AT5G22940.1; AT5G22940.
GeneID832358.
GenomeReviewsGene locus AT5G22940 in contig BA000015_GR.
KEGGath:AT5G22940.
NMPDRfig|3702.1.peg.24462.

Organism-specific databases

TAIRAt5g22940.

Phylogenomic databases

eggNOGKOG1021.
GeneTreeEPGT00070000030424.
HOGENOMHBG319192.
InParanoidQ6NMM8.
OMAASHDFGA.
PhylomeDBQ6NMM8.
ProtClustDBCLSN2681061.

Gene expression databases

GenevestigatorQ6NMM8.

Family and domain databases

InterProIPR004263. Exostosin.
[Graphical view]
PfamPF03016. Exostosin. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF8H_ARATH
AccessionPrimary (citable) accession number: Q6NMM8
Secondary accession number(s): Q9FFB4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: July 5, 2004
Last modified: December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families