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Protein

Phosphoinositide phospholipase C 9

Gene

PLC9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.By similarity

Caution

Contains substitutions of several amino acids thought to be essential for catalytic activity. Its enzyme activity is therefore unsure.Curated

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

GO - Molecular functioni

  • phosphatidylinositol phospholipase C activity Source: TAIR
  • signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  • calcium ion homeostasis Source: TAIR
  • heat acclimation Source: TAIR
  • intracellular signal transduction Source: InterPro
  • lipid catabolic process Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT3G47220-MONOMER
BRENDAi3.1.4.11 399
ReactomeiR-ATH-112043 PLC beta mediated events
R-ATH-114604 GPVI-mediated activation cascade
R-ATH-1855204 Synthesis of IP3 and IP4 in the cytosol
R-ATH-202433 Generation of second messenger molecules
R-ATH-399997 Acetylcholine regulates insulin secretion
R-ATH-416476 G alpha (q) signalling events
R-ATH-434316 Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion
R-ATH-5607764 CLEC7A (Dectin-1) signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 9 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC9
Short name:
AtPLC9
Short name:
PI-PLC9
Gene namesi
Name:PLC9
Ordered Locus Names:At3g47220
ORF Names:F13I12.270
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G47220
TAIRilocus:2075696 AT3G47220

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003241341 – 531Phosphoinositide phospholipase C 9Add BLAST531

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei276PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6NMA7

Expressioni

Tissue specificityi

Expressed in leaves, roots, flowers and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ6NMA7 baseline and differential
GenevisibleiQ6NMA7 AT

Interactioni

Protein-protein interaction databases

BioGridi9195, 13 interactors
STRINGi3702.AT3G47220.1

Structurei

3D structure databases

ProteinModelPortaliQ6NMA7
SMRiQ6NMA7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini107 – 253PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST147
Domaini265 – 385PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST121
Domaini390 – 496C2PROSITE-ProRule annotationAdd BLAST107

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
HOGENOMiHOG000244119
InParanoidiQ6NMA7
OrthoDBiEOG0936050V
PhylomeDBiQ6NMA7

Family and domain databases

Gene3Di2.60.40.150, 1 hit
3.20.20.190, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR001192 PI-PLC_fam
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 2 hits
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q6NMA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNLRKKFEM KQANQPGRVP NYFRNKYHGY DDDMPNLLPT FIKLLDTEKD
60 70 80 90 100
EDGAGLNAAE QIDRELKSRK CDILKFRNLT ILELPHLNEF LFSTELNPPI
110 120 130 140 150
SDQVRHRDMN APLSHYFIHT SLKSYFTGNN VFGRLYSIEP IIDALKQGVR
160 170 180 190 200
VVELDLLPFG KDGICVRPKW NFEKPLELQE CLDAIKQHAF TPTRSYPVII
210 220 230 240 250
TIKDSLKPDL QSKVTQMIDQ TFGDMVYHED PQQSLEEFPS PAELQNKILI
260 270 280 290 300
SRRPPTKLLY AKAVENGVEL EIQEGSTDKN YQSVVGFHAV EPRGMLQKAL
310 320 330 340 350
TDDVQQPGWY ERDVISFTQN KFLRTRPKKR NLLSNPPYKP QRAWMHGAQM
360 370 380 390 400
IALSRQDDKE KLWLMQGMFR ANGGCGYVKK PNFLLNAGSS GVFYPTENPV
410 420 430 440 450
VVKTLKVKIY MGDGWIVDFK KRIGRLSKPD LYVRISIAGV PHDEKIMNTT
460 470 480 490 500
VKNNEWKPTW GEEFTFPLTY PDLALISFEV YDYEVSTPDY FCGQTCLPVS
510 520 530
ELIEGIRAVP LYDERGKACS STMLLTRFKW S
Length:531
Mass (Da):61,165
Last modified:July 5, 2004 - v1
Checksum:i44297407ACADA151
GO

Sequence cautioni

The sequence CAB61968 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133292 Genomic DNA Translation: CAB61968.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE78257.1
BT011755 mRNA Translation: AAS49118.1
AK226689 mRNA Translation: BAE98796.1
PIRiT45658
RefSeqiNP_190306.2, NM_114589.5
UniGeneiAt.66519

Genome annotation databases

EnsemblPlantsiAT3G47220.1; AT3G47220.1; AT3G47220
GeneIDi823875
GrameneiAT3G47220.1; AT3G47220.1; AT3G47220
KEGGiath:AT3G47220

Similar proteinsi

Entry informationi

Entry nameiPLCD9_ARATH
AccessioniPrimary (citable) accession number: Q6NMA7
Secondary accession number(s): Q9SD51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 5, 2004
Last modified: April 25, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health