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Protein

Phosphoinositide phospholipase C 9

Gene

PLC9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

GO - Molecular functioni

  • phosphatidylinositol phospholipase C activity Source: TAIR
  • signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  • calcium ion homeostasis Source: TAIR
  • heat acclimation Source: TAIR
  • intracellular signal transduction Source: InterPro
  • lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT3G47220-MONOMER.
BRENDAi3.1.4.11. 399.
ReactomeiR-ATH-114604. GPVI-mediated activation cascade.
R-ATH-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-ATH-202433. Generation of second messenger molecules.
R-ATH-399997. Acetylcholine regulates insulin secretion.
R-ATH-416476. G alpha (q) signalling events.
R-ATH-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-ATH-5607764. CLEC7A (Dectin-1) signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 9 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC9
Short name:
AtPLC9
Short name:
PI-PLC9
Gene namesi
Name:PLC9
Ordered Locus Names:At3g47220
ORF Names:F13I12.270
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G47220.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: GOC
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003241341 – 531Phosphoinositide phospholipase C 9Add BLAST531

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei276PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6NMA7.

Expressioni

Tissue specificityi

Expressed in leaves, roots, flowers and siliques.1 Publication

Gene expression databases

GenevisibleiQ6NMA7. AT.

Interactioni

Protein-protein interaction databases

BioGridi9195. 13 interactors.
STRINGi3702.AT3G47220.1.

Structurei

3D structure databases

ProteinModelPortaliQ6NMA7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini107 – 253PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST147
Domaini265 – 385PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST121
Domaini390 – 496C2PROSITE-ProRule annotationAdd BLAST107

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
HOGENOMiHOG000244119.
InParanoidiQ6NMA7.
OrthoDBiEOG0936050V.
PhylomeDBiQ6NMA7.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
3.20.20.190. 1 hit.
InterProiIPR000008. C2_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NMA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNLRKKFEM KQANQPGRVP NYFRNKYHGY DDDMPNLLPT FIKLLDTEKD
60 70 80 90 100
EDGAGLNAAE QIDRELKSRK CDILKFRNLT ILELPHLNEF LFSTELNPPI
110 120 130 140 150
SDQVRHRDMN APLSHYFIHT SLKSYFTGNN VFGRLYSIEP IIDALKQGVR
160 170 180 190 200
VVELDLLPFG KDGICVRPKW NFEKPLELQE CLDAIKQHAF TPTRSYPVII
210 220 230 240 250
TIKDSLKPDL QSKVTQMIDQ TFGDMVYHED PQQSLEEFPS PAELQNKILI
260 270 280 290 300
SRRPPTKLLY AKAVENGVEL EIQEGSTDKN YQSVVGFHAV EPRGMLQKAL
310 320 330 340 350
TDDVQQPGWY ERDVISFTQN KFLRTRPKKR NLLSNPPYKP QRAWMHGAQM
360 370 380 390 400
IALSRQDDKE KLWLMQGMFR ANGGCGYVKK PNFLLNAGSS GVFYPTENPV
410 420 430 440 450
VVKTLKVKIY MGDGWIVDFK KRIGRLSKPD LYVRISIAGV PHDEKIMNTT
460 470 480 490 500
VKNNEWKPTW GEEFTFPLTY PDLALISFEV YDYEVSTPDY FCGQTCLPVS
510 520 530
ELIEGIRAVP LYDERGKACS STMLLTRFKW S
Length:531
Mass (Da):61,165
Last modified:July 5, 2004 - v1
Checksum:i44297407ACADA151
GO

Sequence cautioni

The sequence CAB61968 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133292 Genomic DNA. Translation: CAB61968.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78257.1.
BT011755 mRNA. Translation: AAS49118.1.
AK226689 mRNA. Translation: BAE98796.1.
PIRiT45658.
RefSeqiNP_190306.2. NM_114589.5.
UniGeneiAt.66519.

Genome annotation databases

EnsemblPlantsiAT3G47220.1; AT3G47220.1; AT3G47220.
GeneIDi823875.
GrameneiAT3G47220.1; AT3G47220.1; AT3G47220.
KEGGiath:AT3G47220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133292 Genomic DNA. Translation: CAB61968.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78257.1.
BT011755 mRNA. Translation: AAS49118.1.
AK226689 mRNA. Translation: BAE98796.1.
PIRiT45658.
RefSeqiNP_190306.2. NM_114589.5.
UniGeneiAt.66519.

3D structure databases

ProteinModelPortaliQ6NMA7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9195. 13 interactors.
STRINGi3702.AT3G47220.1.

Proteomic databases

PaxDbiQ6NMA7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G47220.1; AT3G47220.1; AT3G47220.
GeneIDi823875.
GrameneiAT3G47220.1; AT3G47220.1; AT3G47220.
KEGGiath:AT3G47220.

Organism-specific databases

TAIRiAT3G47220.

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
HOGENOMiHOG000244119.
InParanoidiQ6NMA7.
OrthoDBiEOG0936050V.
PhylomeDBiQ6NMA7.

Enzyme and pathway databases

BioCyciARA:AT3G47220-MONOMER.
BRENDAi3.1.4.11. 399.
ReactomeiR-ATH-114604. GPVI-mediated activation cascade.
R-ATH-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-ATH-202433. Generation of second messenger molecules.
R-ATH-399997. Acetylcholine regulates insulin secretion.
R-ATH-416476. G alpha (q) signalling events.
R-ATH-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-ATH-5607764. CLEC7A (Dectin-1) signaling.

Miscellaneous databases

PROiQ6NMA7.

Gene expression databases

GenevisibleiQ6NMA7. AT.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
3.20.20.190. 1 hit.
InterProiIPR000008. C2_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLCD9_ARATH
AccessioniPrimary (citable) accession number: Q6NMA7
Secondary accession number(s): Q9SD51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Contains substitutions of several amino acids thought to be essential for catalytic activity. Its enzyme activity is therefore unsure.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.