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Q6NLW5 (XRI1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein XRI1
Alternative name(s):
Protein X-RAY INDUCED 1
Gene names
Name:XRI1
Ordered Locus Names:At5g48720
ORF Names:K24G6.5
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length300 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for mitotic division of the generative cell nucleus and the development of mature tricellular pollen grains, and for male and female meiosis. Ref.1

Subunit structure

Interacts (via C-terminal domain) with MIP1. Ref.1

Subcellular location

Nucleus Ref.1.

Induction

By X-rays and bleomycin treatment. Ref.1 Ref.5

Disruption phenotype

Complete sterility. Ref.1

Miscellaneous

The induction by X-rays is dependent on DNA damage signaling by ATM.

Sequence caution

The sequence BAB09425.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processMeiosis
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from mutant phenotype Ref.1. Source: TAIR

female meiotic division

Inferred from mutant phenotype Ref.1. Source: TAIR

male meiosis

Inferred from mutant phenotype Ref.1. Source: TAIR

pollen development

Inferred from mutant phenotype Ref.1. Source: TAIR

response to X-ray

Inferred from expression pattern Ref.1. Source: TAIR

   Cellular_componentnucleus

Inferred from direct assay Ref.1. Source: TAIR

   Molecular_functionprotein binding

Inferred from physical interaction Ref.1. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MIP1Q8RX222EBI-2270660,EBI-2270543

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6NLW5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 (identifier: Q6NLW5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 300300Protein XRI1
PRO_0000378327

Natural variations

Alternative sequence1 – 3434Missing in isoform 2.
VSP_037542

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 16, 2009. Version 2.
Checksum: 23B32F668BDB6FEA

FASTA30033,719
        10         20         30         40         50         60 
MDYGDDDRSS SWNWQVDNYN HQPQSHFSDV PDCTMTEVTL NQEDHSYMFD DENTPVKACS 

        70         80         90        100        110        120 
ELGYHVTTED TNRKMEVHSE TRSALKRRRM LQFEDQPETS LFSSESFSAI LKSSARDDTF 

       130        140        150        160        170        180 
DELLPEGSQL IEGFSEDASA SSFEGLDLYA EEWYADCLND AETPMLPDDL NFGSPDVQVD 

       190        200        210        220        230        240 
ISEYLNVPPE TETREVQRPV TRSSPNVIFK GRKSFSRPVS KLPSSIIYPF AFIKPCGVHG 

       250        260        270        280        290        300 
GMTLKDINQK IRNPPAKPKA HIEEPAVIQT SAFSGKPVVG KTKIRTEGGK GSITIMRTRG 

« Hide

Isoform 2 [UniParc].

Checksum: C5B4097B2B22F282
Show »

FASTA26629,690

References

« Hide 'large scale' references
[1]"A novel ATM dependant X-ray inducible gene is essential for both plant meiosis and gametogenesis."
Dean P.J., Siwiec T., Waterworth W.M., Schloegelhofer P., Armstrong S.J., West C.E.
Plant J. 58:791-802(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INDUCTION BY X-RAYS, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, INTERACTION WITH MIP1.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[5]"Arabidopsis DNA double-strand break repair pathways."
West C.E., Waterworth W.M., Sunderland P.A., Bray C.M.
Biochem. Soc. Trans. 32:964-966(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY BLEOMYCIN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB012242 Genomic DNA. Translation: BAB09425.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95715.1.
CP002688 Genomic DNA. Translation: AED95716.1.
BT012214 mRNA. Translation: AAS76701.1.
BT020567 mRNA. Translation: AAW78586.1.
RefSeqNP_001154770.1. NM_001161298.1. [Q6NLW5-1]
NP_199683.2. NM_124249.2. [Q6NLW5-2]
UniGeneAt.50516.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ6NLW5. 1 interaction.
STRING3702.AT5G48720.2-P.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G48720.2; AT5G48720.2; AT5G48720. [Q6NLW5-1]
GeneID834930.
KEGGath:AT5G48720.

Organism-specific databases

TAIRAT5G48720.

Phylogenomic databases

eggNOGNOG242961.
HOGENOMHOG000070695.
InParanoidQ6NLW5.
OMAPEEWLVD.
PhylomeDBQ6NLW5.

Gene expression databases

GenevestigatorQ6NLW5.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameXRI1_ARATH
AccessionPrimary (citable) accession number: Q6NLW5
Secondary accession number(s): Q9FKC4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: June 16, 2009
Last modified: July 9, 2014
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names