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Protein

E3 ubiquitin-protein ligase XBAT32

Gene

XBAT32

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of ACC synthases (ACS). Negatively regulates ethylene biosynthesis probably via ubiquitin-dependent degradation of ACS4 and ACS7 enzymes. Regulates lateral root formation and development by controlling ethylene production which inhibits lateral root formation at high concentration.2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri321 – 372RING-typePROSITE-ProRule annotationAdd BLAST52

GO - Molecular functioni

  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • lateral root formation Source: UniProtKB
  • negative regulation of ethylene biosynthetic process Source: UniProtKB
  • protein autoubiquitination Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • response to auxin Source: UniProtKB
  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Molecular functionDevelopmental protein, Transferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase XBAT32 (EC:2.3.2.27)
Alternative name(s):
Ankyrin repeat domain and RING finger-containing protein XBAT32
Protein XB3 homolog 2
RING-type E3 ubiquitin transferase XBAT32
Gene namesi
Name:XBAT32
Ordered Locus Names:At5g57740
ORF Names:MRI1.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRilocus:2172600. AT5G57740.

Pathology & Biotechi

Disruption phenotypei

Delay in development and flowering. Deficiency in lateral root formation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003957401 – 508E3 ubiquitin-protein ligase XBAT32Add BLAST508

Proteomic databases

PaxDbiQ6NLQ8.

Expressioni

Tissue specificityi

Expressed in the vascular system of primary root, vascular tissue of leaves, stems and anthers.1 Publication

Inductioni

By auxin.1 Publication

Gene expression databases

ExpressionAtlasiQ6NLQ8. baseline and differential.
GenevisibleiQ6NLQ8. AT.

Interactioni

Subunit structurei

Interacts with ACS4 and ACS7.1 Publication

Protein-protein interaction databases

BioGridi21126. 4 interactors.
STRINGi3702.AT5G57740.1.

Structurei

3D structure databases

ProteinModelPortaliQ6NLQ8.
SMRiQ6NLQ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati50 – 79ANK 1Add BLAST30
Repeati83 – 112ANK 2Add BLAST30
Repeati117 – 147ANK 3Add BLAST31
Repeati177 – 206ANK 4Add BLAST30
Repeati220 – 249ANK 5Add BLAST30

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri321 – 372RING-typePROSITE-ProRule annotationAdd BLAST52

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IGT9. Eukaryota.
COG0666. LUCA.
HOGENOMiHOG000239563.
InParanoidiQ6NLQ8.
KOiK19044.
OMAiVIFNANI.
OrthoDBiEOG09360AEF.
PhylomeDBiQ6NLQ8.

Family and domain databases

Gene3Di1.25.40.20. 3 hits.
3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF12796. Ank_2. 3 hits.
PRINTSiPR01415. ANKYRIN.
SMARTiView protein in SMART
SM00248. ANK. 5 hits.
SM00184. RING. 1 hit.
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6NLQ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFLSLVGNS FGCSASGERL VSAARDGDLQ EAKALLDYNP RLARYSTFGV
60 70 80 90 100
RNSPLHYSAA QGHHEIVSLL VESGVDINLR NYRGQTALMQ ACQHGHWEVV
110 120 130 140 150
LILILFGANI HRSDYLNGGT ALHLAALNGH PRCIRILLSE YIPSVPNCWS
160 170 180 190 200
LLKNKKTSVA GFDSSVLHEV INRAADGGIT PLHVAALNGH IETVQLLLDL
210 220 230 240 250
GASVTQVTVE DGTTIDLIGA GSTALHYASC GGNTQCCQLL ISKGACLAAV
260 270 280 290 300
NSNGWTPMMV ARSWHRNWLE EILNPTTEQP QLHLPNVPSP FLCLPLMSIV
310 320 330 340 350
NIAQECGWRE NDCLTPCRDP CAVCLERKCT VAADGCAHEF CTNCALYLST
360 370 380 390 400
TSITSSKTSN VTPGSVPCPL CRNGIVSFTK LPHTTATTRT STSSRTSISL
410 420 430 440 450
SFCTCSSDVL DTALLTNPHY SCKPVVSRTG SRTPQSARSS AFRSLSCRRF
460 470 480 490 500
PPSLCLGGSD VDEPRSRLIG GSYSRSGVGF RRSTSQVEGK RSWFSALNHC

VTTGGSAC
Length:508
Mass (Da):54,586
Last modified:July 5, 2004 - v1
Checksum:iD3702C865168E212
GO

Sequence cautioni

The sequence BAB09592 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018118 Genomic DNA. Translation: BAB09592.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96944.1.
BT011688 mRNA. Translation: AAS49051.1.
BT012272 mRNA. Translation: AAS76759.1.
RefSeqiNP_200582.3. NM_125157.3.
UniGeneiAt.29316.

Genome annotation databases

EnsemblPlantsiAT5G57740.1; AT5G57740.1; AT5G57740.
GeneIDi835882.
GrameneiAT5G57740.1; AT5G57740.1; AT5G57740.
KEGGiath:AT5G57740.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018118 Genomic DNA. Translation: BAB09592.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96944.1.
BT011688 mRNA. Translation: AAS49051.1.
BT012272 mRNA. Translation: AAS76759.1.
RefSeqiNP_200582.3. NM_125157.3.
UniGeneiAt.29316.

3D structure databases

ProteinModelPortaliQ6NLQ8.
SMRiQ6NLQ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21126. 4 interactors.
STRINGi3702.AT5G57740.1.

Proteomic databases

PaxDbiQ6NLQ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G57740.1; AT5G57740.1; AT5G57740.
GeneIDi835882.
GrameneiAT5G57740.1; AT5G57740.1; AT5G57740.
KEGGiath:AT5G57740.

Organism-specific databases

AraportiAT5G57740.
TAIRilocus:2172600. AT5G57740.

Phylogenomic databases

eggNOGiENOG410IGT9. Eukaryota.
COG0666. LUCA.
HOGENOMiHOG000239563.
InParanoidiQ6NLQ8.
KOiK19044.
OMAiVIFNANI.
OrthoDBiEOG09360AEF.
PhylomeDBiQ6NLQ8.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiPR:Q6NLQ8.

Gene expression databases

ExpressionAtlasiQ6NLQ8. baseline and differential.
GenevisibleiQ6NLQ8. AT.

Family and domain databases

Gene3Di1.25.40.20. 3 hits.
3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF12796. Ank_2. 3 hits.
PRINTSiPR01415. ANKYRIN.
SMARTiView protein in SMART
SM00248. ANK. 5 hits.
SM00184. RING. 1 hit.
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiXB32_ARATH
AccessioniPrimary (citable) accession number: Q6NLQ8
Secondary accession number(s): Q9FHG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 5, 2004
Last modified: April 12, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.