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Protein

Proline dehydrogenase 2, mitochondrial

Gene

POX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Converts proline to delta-1-pyrroline-5-carboxylate.1 Publication

Catalytic activityi

L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol.

Cofactori

Pathway: L-proline degradation into L-glutamate

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate from L-proline.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Proline dehydrogenase 1, mitochondrial (POX1), Proline dehydrogenase 2, mitochondrial (POX2)
  2. Delta-1-pyrroline-5-carboxylate dehydrogenase 12A1, mitochondrial (ALDH12A1)
This subpathway is part of the pathway L-proline degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from L-proline, the pathway L-proline degradation into L-glutamate and in Amino-acid degradation.

GO - Molecular functioni

  • proline dehydrogenase activity Source: UniProtKB

GO - Biological processi

  • proline catabolic process Source: UniProtKB
  • proline catabolic process to glutamate Source: UniProtKB-UniPathway
  • response to osmotic stress Source: UniProtKB
  • response to water deprivation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Proline metabolism, Stress response

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciARA:AT5G38710-MONOMER.
MetaCyc:AT5G38710-MONOMER.
BRENDAi1.5.99.B2. 399.
ReactomeiREACT_282765. Proline catabolism.
UniPathwayiUPA00261; UER00373.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline dehydrogenase 2, mitochondrial (EC:1.5.5.2)
Alternative name(s):
Osmotic stress-induced proline dehydrogenase
Proline oxidase
Gene namesi
Name:POX2
Synonyms:PRODH2
Ordered Locus Names:At5g38710
ORF Names:MKD10.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G38710.

Subcellular locationi

  • Mitochondrion 1 Publication

GO - Cellular componenti

  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

No visible phenotype when grown under normal conditions. No proline hypersensitivity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2929MitochondrionSequence AnalysisAdd
BLAST
Chaini30 – 476447Proline dehydrogenase 2, mitochondrialPRO_0000394797Add
BLAST

Proteomic databases

PRIDEiQ6NKX1.

Expressioni

Tissue specificityi

Expressed in the vascular tissue and in the abscission zone of petals, sepals, stamina, pistils and siliques. Not detected in petioles.1 Publication

Developmental stagei

Strongly expressed in senescent leaves.1 Publication

Inductioni

Down-regulated by high succrose; via the repression of bZIP11. Up-regulated by proline and salt or drought stress.3 Publications

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G38710.1.

Structurei

3D structure databases

ProteinModelPortaliQ6NKX1.
SMRiQ6NKX1. Positions 211-451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi114 – 1185Poly-Ala

Sequence similaritiesi

Belongs to the proline oxidase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0506.
HOGENOMiHOG000243575.
InParanoidiQ6NKX1.
KOiK00318.
OMAiSKYMPYG.
PhylomeDBiQ6NKX1.

Family and domain databases

Gene3Di3.20.20.220. 2 hits.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR002872. Proline_DH_dom.
IPR015659. Proline_oxidase.
[Graphical view]
PANTHERiPTHR13914. PTHR13914. 1 hit.
PfamiPF01619. Pro_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6NKX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANRFLRPNL IHRFSTVSPV GPPTTIIPEI LSFDQPKPEV DLDLSDQARL
60 70 80 90 100
FASVPISTLL RSTAILHATS IGPMVDLGSW LMSSKLMDTT VTRDLVLRIV
110 120 130 140 150
KGTFYDHFCA GEDAAAAARR VSSVYESTGL KGMLVYGVEH AEDGGACDEN
160 170 180 190 200
IQKFIETVEA AKTLPSSHLS SVVVKITAIC PMNVLKRVSD LLRWQYKNPN
210 220 230 240 250
FKLPWKLNSF PVFSGLSPLY HTTSEPEPLT VEEERELEKA HERLKSVCLR
260 270 280 290 300
CQESNVPLLI DAEDTILQPA IDYMAYWSAI MFNSDKDRPI VYNTIQAYLK
310 320 330 340 350
DAGERLHLAL RESEKMNVPI GFKLVRGAYM SSEAKLADSL GYKSPVHDTI
360 370 380 390 400
QNTHDCYNDC MSFLMEKASN GSGIAVILAT HNTDSGKLGA RKASELGINK
410 420 430 440 450
ENGKIEFAQL YGMSDALSFG LKRAGFNVSK YMPYGPVDTA IPYLIRRAYE
460 470
NRGMMSTGAL DRQLMRKELK RRVMAW
Length:476
Mass (Da):53,074
Last modified:July 5, 2004 - v1
Checksum:iF15FBDF8E06A13F3
GO

Sequence cautioni

The sequence BAB10129.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011478 Genomic DNA. Translation: BAB10129.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED94352.1.
BT012572 mRNA. Translation: AAS99716.1.
AK221601 mRNA. Translation: BAD95150.1.
RefSeqiNP_198687.1. NM_123232.2.
UniGeneiAt.50494.

Genome annotation databases

EnsemblPlantsiAT5G38710.1; AT5G38710.1; AT5G38710.
GeneIDi833862.
KEGGiath:AT5G38710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011478 Genomic DNA. Translation: BAB10129.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED94352.1.
BT012572 mRNA. Translation: AAS99716.1.
AK221601 mRNA. Translation: BAD95150.1.
RefSeqiNP_198687.1. NM_123232.2.
UniGeneiAt.50494.

3D structure databases

ProteinModelPortaliQ6NKX1.
SMRiQ6NKX1. Positions 211-451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G38710.1.

Proteomic databases

PRIDEiQ6NKX1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G38710.1; AT5G38710.1; AT5G38710.
GeneIDi833862.
KEGGiath:AT5G38710.

Organism-specific databases

TAIRiAT5G38710.

Phylogenomic databases

eggNOGiCOG0506.
HOGENOMiHOG000243575.
InParanoidiQ6NKX1.
KOiK00318.
OMAiSKYMPYG.
PhylomeDBiQ6NKX1.

Enzyme and pathway databases

UniPathwayiUPA00261; UER00373.
BioCyciARA:AT5G38710-MONOMER.
MetaCyc:AT5G38710-MONOMER.
BRENDAi1.5.99.B2. 399.
ReactomeiREACT_282765. Proline catabolism.

Miscellaneous databases

PROiQ6NKX1.

Family and domain databases

Gene3Di3.20.20.220. 2 hits.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR002872. Proline_DH_dom.
IPR015659. Proline_oxidase.
[Graphical view]
PANTHERiPTHR13914. PTHR13914. 1 hit.
PfamiPF01619. Pro_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
    Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Arabidopsis ORF clones."
    Shinn P., Chen H., Cheuk R., Kim C.J., Carninci P., Hayashizaki Y., Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M., Shinozaki K., Ecker J.R.
    Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Two tobacco proline dehydrogenases are differentially regulated and play a role in early plant development."
    Ribarits A., Abdullaev A., Tashpulatov A., Richter A., Heberle-Bors E., Touraev A.
    Planta 225:1313-1324(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "The sucrose regulated transcription factor bZIP11 affects amino acid metabolism by regulating the expression of ASPARAGINE SYNTHETASE1 and PROLINE DEHYDROGENASE2."
    Hanson J., Hanssen M., Wiese A., Hendriks M.M., Smeekens S.
    Plant J. 53:935-949(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Non-redundant functions of two proline dehydrogenase isoforms in Arabidopsis."
    Funck D., Eckard S., Mueller G.
    BMC Plant Biol. 10:70-70(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPROD2_ARATH
AccessioniPrimary (citable) accession number: Q6NKX1
Secondary accession number(s): Q9FKR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: July 5, 2004
Last modified: June 24, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.