Q6NKX1 (PROD2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline dehydrogenase 2, mitochondrial
EC=1.5.99.8

Alternative name(s):
Osmotic stress-induced proline dehydrogenase
Proline oxidase

Gene names

Name:	POX2
Synonyms:	PRODH2
Ordered Locus Names:	At5g38710
ORF Names:	MKD10.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts proline to delta-1-pyrroline-5-carboxylate. Ref.7
Catalytic activity	L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor.
Cofactor	FAD.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.
Subcellular location	Mitochondrion Ref.7.
Tissue specificity	Expressed in the vascular tissue and in the abscission zone of petals, sepals, stamina, pistils and siliques. Not detected in petioles. Ref.7
Developmental stage	Strongly expressed in senescent leaves. Ref.7
Induction	Down-regulated by high succrose; via the repression of bZIP11. Up-regulated by proline and salt or drought stress. Ref.5 Ref.6 Ref.7
Disruption phenotype	No visible phenotype when grown under normal conditions. No proline hypersensitivity. Ref.7
Sequence similarities	Belongs to the proline oxidase family.
Sequence caution	The sequence BAB10129.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Proline metabolism Stress response
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamate biosynthetic process Inferred from electronic annotation. Source: InterPro proline catabolic process Inferred from genetic interaction Ref.7. Source: UniProtKB proline catabolic process to glutamate Inferred from electronic annotation. Source: UniProtKB-UniPathway response to osmotic stress Inferred from expression pattern Ref.7. Source: UniProtKB response to water deprivation Inferred from expression pattern Ref.7. Source: UniProtKB
Cellular_component	mitochondrion Inferred from direct assay Ref.7. Source: UniProtKB
Molecular_function	proline dehydrogenase activity Inferred from genetic interaction Ref.7. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 29	29	Mitochondrion Potential
	Chain	30 – 476	447	Proline dehydrogenase 2, mitochondrial		PRO_0000394797
Regions
	Compositional bias	114 – 118	5	Poly-Ala

Sequences

	Sequence		Length	Mass (Da)
	Q6NKX1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: F15FBDF8E06A13F3	FASTA	476	53,074
	10 20 30 40 50 60 MANRFLRPNL IHRFSTVSPV GPPTTIIPEI LSFDQPKPEV DLDLSDQARL FASVPISTLL 70 80 90 100 110 120 RSTAILHATS IGPMVDLGSW LMSSKLMDTT VTRDLVLRIV KGTFYDHFCA GEDAAAAARR 130 140 150 160 170 180 VSSVYESTGL KGMLVYGVEH AEDGGACDEN IQKFIETVEA AKTLPSSHLS SVVVKITAIC 190 200 210 220 230 240 PMNVLKRVSD LLRWQYKNPN FKLPWKLNSF PVFSGLSPLY HTTSEPEPLT VEEERELEKA 250 260 270 280 290 300 HERLKSVCLR CQESNVPLLI DAEDTILQPA IDYMAYWSAI MFNSDKDRPI VYNTIQAYLK 310 320 330 340 350 360 DAGERLHLAL RESEKMNVPI GFKLVRGAYM SSEAKLADSL GYKSPVHDTI QNTHDCYNDC 370 380 390 400 410 420 MSFLMEKASN GSGIAVILAT HNTDSGKLGA RKASELGINK ENGKIEFAQL YGMSDALSFG 430 440 450 460 470 LKRAGFNVSK YMPYGPVDTA IPYLIRRAYE NRGMMSTGAL DRQLMRKELK RRVMAW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones." Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Shinn P., Chen H., Cheuk R., Kim C.J., Carninci P., Hayashizaki Y., Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M., Shinozaki K., Ecker J.R. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Two tobacco proline dehydrogenases are differentially regulated and play a role in early plant development." Ribarits A., Abdullaev A., Tashpulatov A., Richter A., Heberle-Bors E., Touraev A. Planta 225:1313-1324(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[6]	"The sucrose regulated transcription factor bZIP11 affects amino acid metabolism by regulating the expression of ASPARAGINE SYNTHETASE1 and PROLINE DEHYDROGENASE2." Hanson J., Hanssen M., Wiese A., Hendriks M.M., Smeekens S. Plant J. 53:935-949(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[7]	"Non-redundant functions of two proline dehydrogenase isoforms in Arabidopsis." Funck D., Eckard S., Mueller G. BMC Plant Biol. 10:70-70(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB011478 Genomic DNA. Translation: BAB10129.1. Sequence problems. CP002688 Genomic DNA. Translation: AED94352.1. BT012572 mRNA. Translation: AAS99716.1. AK221601 mRNA. Translation: BAD95150.1.
IPI	IPI00544454.
RefSeq	NP_198687.1. NM_123232.2.
UniGene	At.50494.
3D structure databases
ProteinModelPortal	Q6NKX1.
SMR	Q6NKX1. Positions 211-451.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G38710.1-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G38710.1; AT5G38710.1; AT5G38710.
GeneID	833862.
KEGG	ath:AT5G38710.
Organism-specific databases
TAIR	At5g38710.
Phylogenomic databases
eggNOG	COG0506.
HOGENOM	HOG000243575.
InParanoid	Q6NKX1.
KO	K00318.
OMA	PIGFKLV.
PhylomeDB	Q6NKX1.
ProtClustDB	PLN02681.
Enzyme and pathway databases
BioCyc	ARA:AT5G38710-MONOMER. MetaCyc:AT5G38710-MONOMER.
UniPathway	UPA00261; UER00373.
Gene expression databases
Genevestigator	Q6NKX1.
Family and domain databases
InterPro	IPR002872. Proline_DH. IPR015659. Proline_oxidase. [Graphical view]
PANTHER	PTHR13914. PTHR13914. 1 hit.
Pfam	PF01619. Pro_dh. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROD2_ARATH

Accession

Primary (citable) accession number: Q6NKX1
Secondary accession number(s): Q9FKR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program