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Protein

Proline dehydrogenase 2, mitochondrial

Gene

POX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Converts proline to delta-1-pyrroline-5-carboxylate.1 Publication

Catalytic activityi

L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol.

Cofactori

Pathwayi: L-proline degradation into L-glutamate

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate from L-proline.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Proline dehydrogenase 1, mitochondrial (POX1), Proline dehydrogenase 2, mitochondrial (POX2)
  2. Delta-1-pyrroline-5-carboxylate dehydrogenase 12A1, mitochondrial (ALDH12A1)
This subpathway is part of the pathway L-proline degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from L-proline, the pathway L-proline degradation into L-glutamate and in Amino-acid degradation.

GO - Molecular functioni

  • FAD binding Source: GO_Central
  • proline dehydrogenase activity Source: UniProtKB

GO - Biological processi

  • proline catabolic process Source: UniProtKB
  • proline catabolic process to glutamate Source: GO_Central
  • response to osmotic stress Source: UniProtKB
  • response to water deprivation Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
Biological processProline metabolism, Stress response
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciARA:AT5G38710-MONOMER
MetaCyc:AT5G38710-MONOMER
BRENDAi1.5.99.B2 399
ReactomeiR-ATH-389661 Glyoxylate metabolism and glycine degradation
R-ATH-70688 Proline catabolism
UniPathwayiUPA00261; UER00373

Names & Taxonomyi

Protein namesi
Recommended name:
Proline dehydrogenase 2, mitochondrial (EC:1.5.5.2)
Alternative name(s):
Osmotic stress-induced proline dehydrogenase
Proline oxidase
Gene namesi
Name:POX2
Synonyms:PRODH2
Ordered Locus Names:At5g38710
ORF Names:MKD10.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G38710
TAIRilocus:2166645 AT5G38710

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

No visible phenotype when grown under normal conditions. No proline hypersensitivity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29MitochondrionSequence analysisAdd BLAST29
ChainiPRO_000039479730 – 476Proline dehydrogenase 2, mitochondrialAdd BLAST447

Proteomic databases

PaxDbiQ6NKX1

PTM databases

iPTMnetiQ6NKX1

Expressioni

Tissue specificityi

Expressed in the vascular tissue and in the abscission zone of petals, sepals, stamina, pistils and siliques. Not detected in petioles.1 Publication

Developmental stagei

Strongly expressed in senescent leaves.1 Publication

Inductioni

Down-regulated by high succrose; via the repression of bZIP11. Up-regulated by proline and salt or drought stress.3 Publications

Gene expression databases

ExpressionAtlasiQ6NKX1 baseline and differential
GenevisibleiQ6NKX1 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G38710.1

Structurei

3D structure databases

ProteinModelPortaliQ6NKX1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi114 – 118Poly-Ala5

Sequence similaritiesi

Belongs to the proline oxidase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0186 Eukaryota
COG0506 LUCA
HOGENOMiHOG000243575
InParanoidiQ6NKX1
KOiK00318
OMAiKYVPWGP
OrthoDBiEOG09360J6A
PhylomeDBiQ6NKX1

Family and domain databases

InterProiView protein in InterPro
IPR029041 FAD-linked_oxidoreductase-like
IPR002872 Proline_DH_dom
IPR015659 Proline_oxidase
PANTHERiPTHR13914 PTHR13914, 1 hit
PfamiView protein in Pfam
PF01619 Pro_dh, 1 hit
SUPFAMiSSF51730 SSF51730, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6NKX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANRFLRPNL IHRFSTVSPV GPPTTIIPEI LSFDQPKPEV DLDLSDQARL
60 70 80 90 100
FASVPISTLL RSTAILHATS IGPMVDLGSW LMSSKLMDTT VTRDLVLRIV
110 120 130 140 150
KGTFYDHFCA GEDAAAAARR VSSVYESTGL KGMLVYGVEH AEDGGACDEN
160 170 180 190 200
IQKFIETVEA AKTLPSSHLS SVVVKITAIC PMNVLKRVSD LLRWQYKNPN
210 220 230 240 250
FKLPWKLNSF PVFSGLSPLY HTTSEPEPLT VEEERELEKA HERLKSVCLR
260 270 280 290 300
CQESNVPLLI DAEDTILQPA IDYMAYWSAI MFNSDKDRPI VYNTIQAYLK
310 320 330 340 350
DAGERLHLAL RESEKMNVPI GFKLVRGAYM SSEAKLADSL GYKSPVHDTI
360 370 380 390 400
QNTHDCYNDC MSFLMEKASN GSGIAVILAT HNTDSGKLGA RKASELGINK
410 420 430 440 450
ENGKIEFAQL YGMSDALSFG LKRAGFNVSK YMPYGPVDTA IPYLIRRAYE
460 470
NRGMMSTGAL DRQLMRKELK RRVMAW
Length:476
Mass (Da):53,074
Last modified:July 5, 2004 - v1
Checksum:iF15FBDF8E06A13F3
GO

Sequence cautioni

The sequence BAB10129 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011478 Genomic DNA Translation: BAB10129.1 Sequence problems.
CP002688 Genomic DNA Translation: AED94352.1
BT012572 mRNA Translation: AAS99716.1
AK221601 mRNA Translation: BAD95150.1
RefSeqiNP_198687.1, NM_123232.3
UniGeneiAt.50494

Genome annotation databases

EnsemblPlantsiAT5G38710.1; AT5G38710.1; AT5G38710
GeneIDi833862
GrameneiAT5G38710.1; AT5G38710.1; AT5G38710
KEGGiath:AT5G38710

Similar proteinsi

Entry informationi

Entry nameiPROD2_ARATH
AccessioniPrimary (citable) accession number: Q6NKX1
Secondary accession number(s): Q9FKR5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: July 5, 2004
Last modified: April 25, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health