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Protein

Glucan endo-1,3-beta-glucosidase 8

Gene

At1g64760

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei265NucleophileBy similarity1
Active sitei326NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • defense response Source: UniProtKB-KW
  • plant-type cell wall organization Source: TAIR

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCell wall biogenesis/degradation, Plant defense

Enzyme and pathway databases

BioCyciARA:AT1G64760-MONOMER

Protein family/group databases

CAZyiCBM43 Carbohydrate-Binding Module Family 43
GH17 Glycoside Hydrolase Family 17

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 8 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 8
Short name:
(1->3)-beta-glucanase 8
Beta-1,3-endoglucanase 8
Short name:
Beta-1,3-glucanase 8
Gene namesi
Ordered Locus Names:At1g64760
ORF Names:F13O11.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G64760
TAIRilocus:2010916 AT1G64760

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000025125534 – 455Glucan endo-1,3-beta-glucosidase 8Add BLAST422
PropeptideiPRO_0000251256456 – 481Removed in mature formSequence analysisAdd BLAST26

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi99N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi110N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi126N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi131N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi367 ↔ 428By similarity
Glycosylationi409N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi440N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi455GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

Contains two additional disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ6NKW9
PRIDEiQ6NKW9

Expressioni

Gene expression databases

ExpressionAtlasiQ6NKW9 baseline and differential
GenevisibleiQ6NKW9 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G64760.1

Structurei

3D structure databases

ProteinModelPortaliQ6NKW9
SMRiQ6NKW9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410II84 Eukaryota
ENOG410YARB LUCA
HOGENOMiHOG000238221
InParanoidiQ6NKW9
OMAiQATHKLP
OrthoDBiEOG09360BL5
PhylomeDBiQ6NKW9

Family and domain databases

InterProiView protein in InterPro
IPR000490 Glyco_hydro_17
IPR017853 Glycoside_hydrolase_SF
IPR012946 X8
PfamiView protein in Pfam
PF00332 Glyco_hydro_17, 1 hit
PF07983 X8, 1 hit
SMARTiView protein in SMART
SM00768 X8, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00587 GLYCOSYL_HYDROL_F17, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6NKW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLLALVVG FVIVIGHLGI LVNGLGVNWG TMATHKLPPK TVVQMLKDNN
60 70 80 90 100
INKVKLFDAD ETTMGALAGS GLEVMVAIPN DQLKVMTSYD RAKDWVRKNV
110 120 130 140 150
TRYNFDGGVN ITFVAVGNEP FLKSYNGSFI NLTFPALANI QNALNEAGLG
160 170 180 190 200
NSVKATVPLN ADVYDSPASN PVPSAGRFRP DIIGQMTQIV DFLGKNNAPI
210 220 230 240 250
TINIYPFLSL YGNDDFPLNY AFFDGAEPIN DNGIDYTNVF DANFDTLVSS
260 270 280 290 300
LKAVGHGDMP IIVGEVGWPT EGDKHANAGS AYRFYNGLLP RLGTNKGTPL
310 320 330 340 350
RPTYIEVYLF GLLDEDAKSI APGPFERHWG IFKFDGQPKF PIDLSGQGQS
360 370 380 390 400
KFLIGAQNVP YLPNKWCTFN PEAKDLTKLA ANIDYACTFS DCTALGYGSS
410 420 430 440 450
CNTLDANGNA SYAFNMFFQV KNQDESACYF QGLATITTQN ISQGQCNFPI
460 470 480
QIVASSASSF SCSSYSLVVL IVWFLLSGMM F
Length:481
Mass (Da):52,281
Last modified:October 3, 2006 - v2
Checksum:i01A7938FBF3C65F0
GO

Sequence cautioni

The sequence AAD38251 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95W → L in AAS99718 (Ref. 3) Curated1
Sequence conflicti95W → L in BAD95084 (Ref. 4) Curated1
Sequence conflicti358N → H in AAS99718 (Ref. 3) Curated1
Sequence conflicti358N → H in BAD95084 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006193 Genomic DNA Translation: AAD38251.1 Different initiation.
CP002684 Genomic DNA Translation: AEE34283.1
CP002684 Genomic DNA Translation: AEE34284.1
BT012574 mRNA Translation: AAS99718.1
AK220635 mRNA Translation: BAD95084.1
AK176856 mRNA Translation: BAD44619.1
AK175510 mRNA Translation: BAD43273.1
PIRiG96670
RefSeqiNP_001031232.1, NM_001036155.3
NP_176656.1, NM_105150.3
UniGeneiAt.50002

Genome annotation databases

EnsemblPlantsiAT1G64760.1; AT1G64760.1; AT1G64760
AT1G64760.2; AT1G64760.2; AT1G64760
GeneIDi842784
GrameneiAT1G64760.1; AT1G64760.1; AT1G64760
AT1G64760.2; AT1G64760.2; AT1G64760
KEGGiath:AT1G64760

Similar proteinsi

Entry informationi

Entry nameiE138_ARATH
AccessioniPrimary (citable) accession number: Q6NKW9
Secondary accession number(s): Q67XG3, Q9XIR7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 3, 2006
Last modified: May 23, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health