Q6NKW9 (E138_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucan endo-1,3-beta-glucosidase 8
EC=3.2.1.39

Alternative name(s):
(1->3)-beta-glucan endohydrolase 8
Short name=(1->3)-beta-glucanase 8
Beta-1,3-endoglucanase 8
Short name=Beta-1,3-glucanase 8

Gene names

Ordered Locus Names:	At1g64760
ORF Names:	F13O11.7

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Secreted › cell wall Potential. Cell membrane; Lipid-anchor › GPI-anchor; Extracellular side Ref.5 Ref.6.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.
Sequence caution	The sequence AAD38251.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Plant defense
Cellular component	Cell membrane Cell wall Membrane Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to plasma membrane Inferred from direct assay Ref.5. Source: TAIR cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW plasmodesma Inferred from direct assay. Source: TAIR
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Chain	34 – 455	422	Glucan endo-1,3-beta-glucosidase 8		PRO_0000251255
	Propeptide	456 – 481	26	Removed in mature form Potential		PRO_0000251256
Sites
	Active site	265	1	Nucleophile By similarity
	Active site	326	1	Nucleophile By similarity
Amino acid modifications
	Lipidation	455	1	GPI-anchor amidated serine Potential
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	110	1	N-linked (GlcNAc...) Potential
	Glycosylation	126	1	N-linked (GlcNAc...) Potential
	Glycosylation	131	1	N-linked (GlcNAc...) Potential
	Glycosylation	409	1	N-linked (GlcNAc...) Potential
	Glycosylation	440	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	95	1	W → L in AAS99718. Ref.3
	Sequence conflict	95	1	W → L in BAD95084. Ref.4
	Sequence conflict	358	1	N → H in AAS99718. Ref.3
	Sequence conflict	358	1	N → H in BAD95084. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q6NKW9 [UniParc]. Last modified October 3, 2006. Version 2. Checksum: 01A7938FBF3C65F0	FASTA	481	52,281
	10 20 30 40 50 60 MSNLLALVVG FVIVIGHLGI LVNGLGVNWG TMATHKLPPK TVVQMLKDNN INKVKLFDAD 70 80 90 100 110 120 ETTMGALAGS GLEVMVAIPN DQLKVMTSYD RAKDWVRKNV TRYNFDGGVN ITFVAVGNEP 130 140 150 160 170 180 FLKSYNGSFI NLTFPALANI QNALNEAGLG NSVKATVPLN ADVYDSPASN PVPSAGRFRP 190 200 210 220 230 240 DIIGQMTQIV DFLGKNNAPI TINIYPFLSL YGNDDFPLNY AFFDGAEPIN DNGIDYTNVF 250 260 270 280 290 300 DANFDTLVSS LKAVGHGDMP IIVGEVGWPT EGDKHANAGS AYRFYNGLLP RLGTNKGTPL 310 320 330 340 350 360 RPTYIEVYLF GLLDEDAKSI APGPFERHWG IFKFDGQPKF PIDLSGQGQS KFLIGAQNVP 370 380 390 400 410 420 YLPNKWCTFN PEAKDLTKLA ANIDYACTFS DCTALGYGSS CNTLDANGNA SYAFNMFFQV 430 440 450 460 470 480 KNQDESACYF QGLATITTQN ISQGQCNFPI QIVASSASSF SCSSYSLVVL IVWFLLSGMM F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	Shinn P., Chen H., Cheuk R.F., Kim C.J., Carninci P., Hayashizaki Y., Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M., Shinozaki K., Ecker J.R. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins." Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N. Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed: 14517339] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment." Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N. J. Proteome Res. 5:935-943(2006) [PubMed: 16602701] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006193 Genomic DNA. Translation: AAD38251.1. Different initiation. CP002684 Genomic DNA. Translation: AEE34283.1. CP002684 Genomic DNA. Translation: AEE34284.1. BT012574 mRNA. Translation: AAS99718.1. AK220635 mRNA. Translation: BAD95084.1. AK176856 mRNA. Translation: BAD44619.1. AK175510 mRNA. Translation: BAD43273.1.
IPI	IPI00535099.
PIR	G96670.
RefSeq	NP_001031232.1. NM_001036155.2. NP_176656.1. NM_105150.2.
UniGene	At.50002.
3D structure databases
HSSP	HSSP built from PDB template 2CYG based on UniProtKB O22317.
ProteinModelPortal	Q6NKW9.
SMR	Q6NKW9. Positions 25-344, 357-451.
ModBase	Search...
Protein family/group databases
CAZy	CBM43. Carbohydrate-Binding Module Family 43. GH17. Glycoside Hydrolase Family 17.
Proteomic databases
PRIDE	Q6NKW9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G64760.1; AT1G64760.1; AT1G64760. AT1G64760.2; AT1G64760.2; AT1G64760.
GeneID	842784.
GenomeReviews	Gene locus AT1G64760 in contig CT485782_GR.
KEGG	ath:AT1G64760.
NMPDR	fig|3702.1.peg.5894.
Organism-specific databases
TAIR	At1g64760.
Phylogenomic databases
GeneTree	EPGT00050000009360.
HOGENOM	HBG605194.
InParanoid	Q6NKW9.
OMA	LYGNDNF.
PhylomeDB	Q6NKW9.
ProtClustDB	CLSN2914646.
Gene expression databases
Genevestigator	Q6NKW9.
GermOnline	AT1G64760. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR012946. X8. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. PF07983. X8. 1 hit. [Graphical view]
SMART	SM00768. X8. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E138_ARATH

Accession

Primary (citable) accession number: Q6NKW9
Secondary accession number(s): Q67XG3, Q9XIR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	October 3, 2006
Last modified:	December 14, 2011
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program