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Protein

Glucan endo-1,3-beta-glucosidase 8

Gene

At1g64760

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei265 – 2651NucleophileBy similarity
Active sitei326 – 3261NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Plant defense

Enzyme and pathway databases

BioCyciARA:AT1G64760-MONOMER.
ARA:GQT-2182-MONOMER.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 8 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 8
Short name:
(1->3)-beta-glucanase 8
Beta-1,3-endoglucanase 8
Short name:
Beta-1,3-glucanase 8
Gene namesi
Ordered Locus Names:At1g64760
ORF Names:F13O11.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G64760.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: TAIR
  • anchored component of plasma membrane Source: TAIR
  • cell wall Source: UniProtKB-SubCell
  • extracellular region Source: UniProtKB-KW
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence AnalysisAdd
BLAST
Chaini34 – 455422Glucan endo-1,3-beta-glucosidase 8PRO_0000251255Add
BLAST
Propeptidei456 – 48126Removed in mature formSequence AnalysisPRO_0000251256Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi367 ↔ 428By similarity
Glycosylationi409 – 4091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi440 – 4401N-linked (GlcNAc...)Sequence Analysis
Lipidationi455 – 4551GPI-anchor amidated serineSequence Analysis

Post-translational modificationi

Contains two additional disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ6NKW9.
PRIDEiQ6NKW9.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G64760.1.

Structurei

3D structure databases

ProteinModelPortaliQ6NKW9.
SMRiQ6NKW9. Positions 25-344, 360-449.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG247729.
HOGENOMiHOG000238221.
InParanoidiQ6NKW9.
OMAiDACNFTI.
PhylomeDBiQ6NKW9.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6NKW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLLALVVG FVIVIGHLGI LVNGLGVNWG TMATHKLPPK TVVQMLKDNN
60 70 80 90 100
INKVKLFDAD ETTMGALAGS GLEVMVAIPN DQLKVMTSYD RAKDWVRKNV
110 120 130 140 150
TRYNFDGGVN ITFVAVGNEP FLKSYNGSFI NLTFPALANI QNALNEAGLG
160 170 180 190 200
NSVKATVPLN ADVYDSPASN PVPSAGRFRP DIIGQMTQIV DFLGKNNAPI
210 220 230 240 250
TINIYPFLSL YGNDDFPLNY AFFDGAEPIN DNGIDYTNVF DANFDTLVSS
260 270 280 290 300
LKAVGHGDMP IIVGEVGWPT EGDKHANAGS AYRFYNGLLP RLGTNKGTPL
310 320 330 340 350
RPTYIEVYLF GLLDEDAKSI APGPFERHWG IFKFDGQPKF PIDLSGQGQS
360 370 380 390 400
KFLIGAQNVP YLPNKWCTFN PEAKDLTKLA ANIDYACTFS DCTALGYGSS
410 420 430 440 450
CNTLDANGNA SYAFNMFFQV KNQDESACYF QGLATITTQN ISQGQCNFPI
460 470 480
QIVASSASSF SCSSYSLVVL IVWFLLSGMM F
Length:481
Mass (Da):52,281
Last modified:October 3, 2006 - v2
Checksum:i01A7938FBF3C65F0
GO

Sequence cautioni

The sequence AAD38251.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 951W → L in AAS99718 (Ref. 3) Curated
Sequence conflicti95 – 951W → L in BAD95084 (Ref. 4) Curated
Sequence conflicti358 – 3581N → H in AAS99718 (Ref. 3) Curated
Sequence conflicti358 – 3581N → H in BAD95084 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006193 Genomic DNA. Translation: AAD38251.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE34283.1.
CP002684 Genomic DNA. Translation: AEE34284.1.
BT012574 mRNA. Translation: AAS99718.1.
AK220635 mRNA. Translation: BAD95084.1.
AK176856 mRNA. Translation: BAD44619.1.
AK175510 mRNA. Translation: BAD43273.1.
PIRiG96670.
RefSeqiNP_001031232.1. NM_001036155.2.
NP_176656.1. NM_105150.2.
UniGeneiAt.50002.

Genome annotation databases

EnsemblPlantsiAT1G64760.1; AT1G64760.1; AT1G64760.
AT1G64760.2; AT1G64760.2; AT1G64760.
GeneIDi842784.
KEGGiath:AT1G64760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006193 Genomic DNA. Translation: AAD38251.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE34283.1.
CP002684 Genomic DNA. Translation: AEE34284.1.
BT012574 mRNA. Translation: AAS99718.1.
AK220635 mRNA. Translation: BAD95084.1.
AK176856 mRNA. Translation: BAD44619.1.
AK175510 mRNA. Translation: BAD43273.1.
PIRiG96670.
RefSeqiNP_001031232.1. NM_001036155.2.
NP_176656.1. NM_105150.2.
UniGeneiAt.50002.

3D structure databases

ProteinModelPortaliQ6NKW9.
SMRiQ6NKW9. Positions 25-344, 360-449.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G64760.1.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbiQ6NKW9.
PRIDEiQ6NKW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G64760.1; AT1G64760.1; AT1G64760.
AT1G64760.2; AT1G64760.2; AT1G64760.
GeneIDi842784.
KEGGiath:AT1G64760.

Organism-specific databases

TAIRiAT1G64760.

Phylogenomic databases

eggNOGiNOG247729.
HOGENOMiHOG000238221.
InParanoidiQ6NKW9.
OMAiDACNFTI.
PhylomeDBiQ6NKW9.

Enzyme and pathway databases

BioCyciARA:AT1G64760-MONOMER.
ARA:GQT-2182-MONOMER.

Miscellaneous databases

PROiQ6NKW9.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
    Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
    Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
  6. "Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment."
    Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N.
    J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiE138_ARATH
AccessioniPrimary (citable) accession number: Q6NKW9
Secondary accession number(s): Q67XG3, Q9XIR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 3, 2006
Last modified: June 24, 2015
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.