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Q6NKW9

- E138_ARATH

UniProt

Q6NKW9 - E138_ARATH

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Protein

Glucan endo-1,3-beta-glucosidase 8

Gene
At1g64760, F13O11.7
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei265 – 2651Nucleophile By similarity
Active sitei326 – 3261Nucleophile By similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Plant defense

Enzyme and pathway databases

BioCyciARA:AT1G64760-MONOMER.
ARA:GQT-2182-MONOMER.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 8 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 8
Short name:
(1->3)-beta-glucanase 8
Beta-1,3-endoglucanase 8
Short name:
Beta-1,3-glucanase 8
Gene namesi
Ordered Locus Names:At1g64760
ORF Names:F13O11.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G64760.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: TAIR
  2. anchored component of plasma membrane Source: TAIR
  3. cell wall Source: UniProtKB-SubCell
  4. extracellular region Source: UniProtKB-KW
  5. plasma membrane Source: TAIR
  6. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333 Reviewed predictionAdd
BLAST
Chaini34 – 455422Glucan endo-1,3-beta-glucosidase 8PRO_0000251255Add
BLAST
Propeptidei456 – 48126Removed in mature form Reviewed predictionPRO_0000251256Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi99 – 991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi110 – 1101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi126 – 1261N-linked (GlcNAc...) Reviewed prediction
Glycosylationi131 – 1311N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi367 ↔ 428 By similarity
Glycosylationi409 – 4091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi440 – 4401N-linked (GlcNAc...) Reviewed prediction
Lipidationi455 – 4551GPI-anchor amidated serine Reviewed prediction

Post-translational modificationi

Contains two additional disulfide bonds By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ6NKW9.
PRIDEiQ6NKW9.

Expressioni

Gene expression databases

GenevestigatoriQ6NKW9.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G64760.2-P.

Structurei

3D structure databases

ProteinModelPortaliQ6NKW9.
SMRiQ6NKW9. Positions 25-344, 360-449.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG247729.
HOGENOMiHOG000238221.
InParanoidiQ6NKW9.
OMAiTRYNFDG.
PhylomeDBiQ6NKW9.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6NKW9-1 [UniParc]FASTAAdd to Basket

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MSNLLALVVG FVIVIGHLGI LVNGLGVNWG TMATHKLPPK TVVQMLKDNN    50
INKVKLFDAD ETTMGALAGS GLEVMVAIPN DQLKVMTSYD RAKDWVRKNV 100
TRYNFDGGVN ITFVAVGNEP FLKSYNGSFI NLTFPALANI QNALNEAGLG 150
NSVKATVPLN ADVYDSPASN PVPSAGRFRP DIIGQMTQIV DFLGKNNAPI 200
TINIYPFLSL YGNDDFPLNY AFFDGAEPIN DNGIDYTNVF DANFDTLVSS 250
LKAVGHGDMP IIVGEVGWPT EGDKHANAGS AYRFYNGLLP RLGTNKGTPL 300
RPTYIEVYLF GLLDEDAKSI APGPFERHWG IFKFDGQPKF PIDLSGQGQS 350
KFLIGAQNVP YLPNKWCTFN PEAKDLTKLA ANIDYACTFS DCTALGYGSS 400
CNTLDANGNA SYAFNMFFQV KNQDESACYF QGLATITTQN ISQGQCNFPI 450
QIVASSASSF SCSSYSLVVL IVWFLLSGMM F 481
Length:481
Mass (Da):52,281
Last modified:October 3, 2006 - v2
Checksum:i01A7938FBF3C65F0
GO

Sequence cautioni

The sequence AAD38251.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 951W → L in AAS99718. 1 Publication
Sequence conflicti95 – 951W → L in BAD95084. 1 Publication
Sequence conflicti358 – 3581N → H in AAS99718. 1 Publication
Sequence conflicti358 – 3581N → H in BAD95084. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC006193 Genomic DNA. Translation: AAD38251.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE34283.1.
CP002684 Genomic DNA. Translation: AEE34284.1.
BT012574 mRNA. Translation: AAS99718.1.
AK220635 mRNA. Translation: BAD95084.1.
AK176856 mRNA. Translation: BAD44619.1.
AK175510 mRNA. Translation: BAD43273.1.
PIRiG96670.
RefSeqiNP_001031232.1. NM_001036155.2.
NP_176656.1. NM_105150.2.
UniGeneiAt.50002.

Genome annotation databases

EnsemblPlantsiAT1G64760.1; AT1G64760.1; AT1G64760.
AT1G64760.2; AT1G64760.2; AT1G64760.
GeneIDi842784.
KEGGiath:AT1G64760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC006193 Genomic DNA. Translation: AAD38251.1 . Different initiation.
CP002684 Genomic DNA. Translation: AEE34283.1 .
CP002684 Genomic DNA. Translation: AEE34284.1 .
BT012574 mRNA. Translation: AAS99718.1 .
AK220635 mRNA. Translation: BAD95084.1 .
AK176856 mRNA. Translation: BAD44619.1 .
AK175510 mRNA. Translation: BAD43273.1 .
PIRi G96670.
RefSeqi NP_001031232.1. NM_001036155.2.
NP_176656.1. NM_105150.2.
UniGenei At.50002.

3D structure databases

ProteinModelPortali Q6NKW9.
SMRi Q6NKW9. Positions 25-344, 360-449.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G64760.2-P.

Protein family/group databases

CAZyi CBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbi Q6NKW9.
PRIDEi Q6NKW9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G64760.1 ; AT1G64760.1 ; AT1G64760 .
AT1G64760.2 ; AT1G64760.2 ; AT1G64760 .
GeneIDi 842784.
KEGGi ath:AT1G64760.

Organism-specific databases

TAIRi AT1G64760.

Phylogenomic databases

eggNOGi NOG247729.
HOGENOMi HOG000238221.
InParanoidi Q6NKW9.
OMAi TRYNFDG.
PhylomeDBi Q6NKW9.

Enzyme and pathway databases

BioCyci ARA:AT1G64760-MONOMER.
ARA:GQT-2182-MONOMER.

Gene expression databases

Genevestigatori Q6NKW9.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view ]
SMARTi SM00768. X8. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
    Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
    Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
  6. "Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment."
    Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N.
    J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiE138_ARATH
AccessioniPrimary (citable) accession number: Q6NKW9
Secondary accession number(s): Q67XG3, Q9XIR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 3, 2006
Last modified: September 3, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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