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Q6NKW9

- E138_ARATH

UniProt

Q6NKW9 - E138_ARATH

Protein

Glucan endo-1,3-beta-glucosidase 8

Gene

At1g64760

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 2 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei265 – 2651NucleophileBy similarity
    Active sitei326 – 3261NucleophileBy similarity

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation, Plant defense

    Enzyme and pathway databases

    BioCyciARA:AT1G64760-MONOMER.
    ARA:GQT-2182-MONOMER.

    Protein family/group databases

    CAZyiCBM43. Carbohydrate-Binding Module Family 43.
    GH17. Glycoside Hydrolase Family 17.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase 8 (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase 8
    Short name:
    (1->3)-beta-glucanase 8
    Beta-1,3-endoglucanase 8
    Short name:
    Beta-1,3-glucanase 8
    Gene namesi
    Ordered Locus Names:At1g64760
    ORF Names:F13O11.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G64760.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: TAIR
    2. anchored component of plasma membrane Source: TAIR
    3. cell wall Source: UniProtKB-SubCell
    4. extracellular region Source: UniProtKB-KW
    5. plasma membrane Source: TAIR
    6. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Cell wall, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Sequence AnalysisAdd
    BLAST
    Chaini34 – 455422Glucan endo-1,3-beta-glucosidase 8PRO_0000251255Add
    BLAST
    Propeptidei456 – 48126Removed in mature formSequence AnalysisPRO_0000251256Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi367 ↔ 428By similarity
    Glycosylationi409 – 4091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi440 – 4401N-linked (GlcNAc...)Sequence Analysis
    Lipidationi455 – 4551GPI-anchor amidated serineSequence Analysis

    Post-translational modificationi

    Contains two additional disulfide bonds.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ6NKW9.
    PRIDEiQ6NKW9.

    Expressioni

    Gene expression databases

    GenevestigatoriQ6NKW9.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G64760.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6NKW9.
    SMRiQ6NKW9. Positions 25-344, 360-449.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG247729.
    HOGENOMiHOG000238221.
    InParanoidiQ6NKW9.
    OMAiTRYNFDG.
    PhylomeDBiQ6NKW9.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR012946. X8.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    PF07983. X8. 1 hit.
    [Graphical view]
    SMARTiSM00768. X8. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6NKW9-1 [UniParc]FASTAAdd to Basket

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    MSNLLALVVG FVIVIGHLGI LVNGLGVNWG TMATHKLPPK TVVQMLKDNN    50
    INKVKLFDAD ETTMGALAGS GLEVMVAIPN DQLKVMTSYD RAKDWVRKNV 100
    TRYNFDGGVN ITFVAVGNEP FLKSYNGSFI NLTFPALANI QNALNEAGLG 150
    NSVKATVPLN ADVYDSPASN PVPSAGRFRP DIIGQMTQIV DFLGKNNAPI 200
    TINIYPFLSL YGNDDFPLNY AFFDGAEPIN DNGIDYTNVF DANFDTLVSS 250
    LKAVGHGDMP IIVGEVGWPT EGDKHANAGS AYRFYNGLLP RLGTNKGTPL 300
    RPTYIEVYLF GLLDEDAKSI APGPFERHWG IFKFDGQPKF PIDLSGQGQS 350
    KFLIGAQNVP YLPNKWCTFN PEAKDLTKLA ANIDYACTFS DCTALGYGSS 400
    CNTLDANGNA SYAFNMFFQV KNQDESACYF QGLATITTQN ISQGQCNFPI 450
    QIVASSASSF SCSSYSLVVL IVWFLLSGMM F 481
    Length:481
    Mass (Da):52,281
    Last modified:October 3, 2006 - v2
    Checksum:i01A7938FBF3C65F0
    GO

    Sequence cautioni

    The sequence AAD38251.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti95 – 951W → L in AAS99718. 1 PublicationCurated
    Sequence conflicti95 – 951W → L in BAD95084. 1 PublicationCurated
    Sequence conflicti358 – 3581N → H in AAS99718. 1 PublicationCurated
    Sequence conflicti358 – 3581N → H in BAD95084. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006193 Genomic DNA. Translation: AAD38251.1. Different initiation.
    CP002684 Genomic DNA. Translation: AEE34283.1.
    CP002684 Genomic DNA. Translation: AEE34284.1.
    BT012574 mRNA. Translation: AAS99718.1.
    AK220635 mRNA. Translation: BAD95084.1.
    AK176856 mRNA. Translation: BAD44619.1.
    AK175510 mRNA. Translation: BAD43273.1.
    PIRiG96670.
    RefSeqiNP_001031232.1. NM_001036155.2.
    NP_176656.1. NM_105150.2.
    UniGeneiAt.50002.

    Genome annotation databases

    EnsemblPlantsiAT1G64760.1; AT1G64760.1; AT1G64760.
    AT1G64760.2; AT1G64760.2; AT1G64760.
    GeneIDi842784.
    KEGGiath:AT1G64760.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006193 Genomic DNA. Translation: AAD38251.1 . Different initiation.
    CP002684 Genomic DNA. Translation: AEE34283.1 .
    CP002684 Genomic DNA. Translation: AEE34284.1 .
    BT012574 mRNA. Translation: AAS99718.1 .
    AK220635 mRNA. Translation: BAD95084.1 .
    AK176856 mRNA. Translation: BAD44619.1 .
    AK175510 mRNA. Translation: BAD43273.1 .
    PIRi G96670.
    RefSeqi NP_001031232.1. NM_001036155.2.
    NP_176656.1. NM_105150.2.
    UniGenei At.50002.

    3D structure databases

    ProteinModelPortali Q6NKW9.
    SMRi Q6NKW9. Positions 25-344, 360-449.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G64760.2-P.

    Protein family/group databases

    CAZyi CBM43. Carbohydrate-Binding Module Family 43.
    GH17. Glycoside Hydrolase Family 17.

    Proteomic databases

    PaxDbi Q6NKW9.
    PRIDEi Q6NKW9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G64760.1 ; AT1G64760.1 ; AT1G64760 .
    AT1G64760.2 ; AT1G64760.2 ; AT1G64760 .
    GeneIDi 842784.
    KEGGi ath:AT1G64760.

    Organism-specific databases

    TAIRi AT1G64760.

    Phylogenomic databases

    eggNOGi NOG247729.
    HOGENOMi HOG000238221.
    InParanoidi Q6NKW9.
    OMAi TRYNFDG.
    PhylomeDBi Q6NKW9.

    Enzyme and pathway databases

    BioCyci ARA:AT1G64760-MONOMER.
    ARA:GQT-2182-MONOMER.

    Gene expression databases

    Genevestigatori Q6NKW9.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR012946. X8.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    PF07983. X8. 1 hit.
    [Graphical view ]
    SMARTi SM00768. X8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins."
      Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N.
      Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].
    6. "Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment."
      Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N.
      J. Proteome Res. 5:935-943(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiE138_ARATH
    AccessioniPrimary (citable) accession number: Q6NKW9
    Secondary accession number(s): Q67XG3, Q9XIR7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3