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Protein

Glucan endo-1,3-beta-glucosidase 8

Gene

At1g64760

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei265NucleophileBy similarity1
Active sitei326NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Plant defense

Enzyme and pathway databases

BioCyciARA:AT1G64760-MONOMER.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase 8 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase 8
Short name:
(1->3)-beta-glucanase 8
Beta-1,3-endoglucanase 8
Short name:
Beta-1,3-glucanase 8
Gene namesi
Ordered Locus Names:At1g64760
ORF Names:F13O11.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G64760.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: TAIR
  • anchored component of plasma membrane Source: TAIR
  • cell wall Source: UniProtKB-SubCell
  • extracellular region Source: UniProtKB-KW
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000025125534 – 455Glucan endo-1,3-beta-glucosidase 8Add BLAST422
PropeptideiPRO_0000251256456 – 481Removed in mature formSequence analysisAdd BLAST26

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Glycosylationi110N-linked (GlcNAc...)Sequence analysis1
Glycosylationi126N-linked (GlcNAc...)Sequence analysis1
Glycosylationi131N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi367 ↔ 428By similarity
Glycosylationi409N-linked (GlcNAc...)Sequence analysis1
Glycosylationi440N-linked (GlcNAc...)Sequence analysis1
Lipidationi455GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

Contains two additional disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ6NKW9.
PRIDEiQ6NKW9.

Expressioni

Gene expression databases

GenevisibleiQ6NKW9. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G64760.1.

Structurei

3D structure databases

ProteinModelPortaliQ6NKW9.
SMRiQ6NKW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410II84. Eukaryota.
ENOG410YARB. LUCA.
HOGENOMiHOG000238221.
InParanoidiQ6NKW9.
OMAiCNFIIQI.
OrthoDBiEOG09360BL5.
PhylomeDBiQ6NKW9.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6NKW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLLALVVG FVIVIGHLGI LVNGLGVNWG TMATHKLPPK TVVQMLKDNN
60 70 80 90 100
INKVKLFDAD ETTMGALAGS GLEVMVAIPN DQLKVMTSYD RAKDWVRKNV
110 120 130 140 150
TRYNFDGGVN ITFVAVGNEP FLKSYNGSFI NLTFPALANI QNALNEAGLG
160 170 180 190 200
NSVKATVPLN ADVYDSPASN PVPSAGRFRP DIIGQMTQIV DFLGKNNAPI
210 220 230 240 250
TINIYPFLSL YGNDDFPLNY AFFDGAEPIN DNGIDYTNVF DANFDTLVSS
260 270 280 290 300
LKAVGHGDMP IIVGEVGWPT EGDKHANAGS AYRFYNGLLP RLGTNKGTPL
310 320 330 340 350
RPTYIEVYLF GLLDEDAKSI APGPFERHWG IFKFDGQPKF PIDLSGQGQS
360 370 380 390 400
KFLIGAQNVP YLPNKWCTFN PEAKDLTKLA ANIDYACTFS DCTALGYGSS
410 420 430 440 450
CNTLDANGNA SYAFNMFFQV KNQDESACYF QGLATITTQN ISQGQCNFPI
460 470 480
QIVASSASSF SCSSYSLVVL IVWFLLSGMM F
Length:481
Mass (Da):52,281
Last modified:October 3, 2006 - v2
Checksum:i01A7938FBF3C65F0
GO

Sequence cautioni

The sequence AAD38251 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95W → L in AAS99718 (Ref. 3) Curated1
Sequence conflicti95W → L in BAD95084 (Ref. 4) Curated1
Sequence conflicti358N → H in AAS99718 (Ref. 3) Curated1
Sequence conflicti358N → H in BAD95084 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006193 Genomic DNA. Translation: AAD38251.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE34283.1.
CP002684 Genomic DNA. Translation: AEE34284.1.
BT012574 mRNA. Translation: AAS99718.1.
AK220635 mRNA. Translation: BAD95084.1.
AK176856 mRNA. Translation: BAD44619.1.
AK175510 mRNA. Translation: BAD43273.1.
PIRiG96670.
RefSeqiNP_001031232.1. NM_001036155.3.
NP_176656.1. NM_105150.3.
UniGeneiAt.50002.

Genome annotation databases

EnsemblPlantsiAT1G64760.1; AT1G64760.1; AT1G64760.
AT1G64760.2; AT1G64760.2; AT1G64760.
GeneIDi842784.
GrameneiAT1G64760.1; AT1G64760.1; AT1G64760.
AT1G64760.2; AT1G64760.2; AT1G64760.
KEGGiath:AT1G64760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006193 Genomic DNA. Translation: AAD38251.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE34283.1.
CP002684 Genomic DNA. Translation: AEE34284.1.
BT012574 mRNA. Translation: AAS99718.1.
AK220635 mRNA. Translation: BAD95084.1.
AK176856 mRNA. Translation: BAD44619.1.
AK175510 mRNA. Translation: BAD43273.1.
PIRiG96670.
RefSeqiNP_001031232.1. NM_001036155.3.
NP_176656.1. NM_105150.3.
UniGeneiAt.50002.

3D structure databases

ProteinModelPortaliQ6NKW9.
SMRiQ6NKW9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G64760.1.

Protein family/group databases

CAZyiCBM43. Carbohydrate-Binding Module Family 43.
GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbiQ6NKW9.
PRIDEiQ6NKW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G64760.1; AT1G64760.1; AT1G64760.
AT1G64760.2; AT1G64760.2; AT1G64760.
GeneIDi842784.
GrameneiAT1G64760.1; AT1G64760.1; AT1G64760.
AT1G64760.2; AT1G64760.2; AT1G64760.
KEGGiath:AT1G64760.

Organism-specific databases

TAIRiAT1G64760.

Phylogenomic databases

eggNOGiENOG410II84. Eukaryota.
ENOG410YARB. LUCA.
HOGENOMiHOG000238221.
InParanoidiQ6NKW9.
OMAiCNFIIQI.
OrthoDBiEOG09360BL5.
PhylomeDBiQ6NKW9.

Enzyme and pathway databases

BioCyciARA:AT1G64760-MONOMER.

Miscellaneous databases

PROiQ6NKW9.

Gene expression databases

GenevisibleiQ6NKW9. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR012946. X8.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
PF07983. X8. 1 hit.
[Graphical view]
SMARTiSM00768. X8. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiE138_ARATH
AccessioniPrimary (citable) accession number: Q6NKW9
Secondary accession number(s): Q67XG3, Q9XIR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 3, 2006
Last modified: November 30, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.