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Q6NKS1 (P2C65_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable protein phosphatase 2C 65

Short name=AtPP2C65
EC=3.1.3.16
Gene names
Ordered Locus Names:At5g01700
ORF Names:F7A7.220
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Sequence caution

The sequence BX831823 differs from that shown. Reason: Sequencing errors.

The sequence CAB82286.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   LigandMagnesium
Manganese
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoprotein phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6NKS1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6NKS1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382Probable protein phosphatase 2C 65
PRO_0000367987

Regions

Domain46 – 314269PP2C-like

Sites

Metal binding831Manganese 1 By similarity
Metal binding831Manganese 2 By similarity
Metal binding841Manganese 1; via carbonyl oxygen By similarity
Metal binding2821Manganese 2 By similarity
Metal binding3281Manganese 2 By similarity

Natural variations

Alternative sequence1 – 4949Missing in isoform 2.
VSP_036776

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 27DA3A16DE5E77CA

FASTA38242,004
        10         20         30         40         50         60 
MGVCCSKGTG IIVEHGADDG NECGDGEAEV RDTNDGAVVR TRGSSKHVSM SIKQGKKGIN 

        70         80         90        100        110        120 
QDAMTVWENF GGEEDTIFCG VFDGHGPMGH KISRHVCENL PSRVHSKIRS SKSAGDENIE 

       130        140        150        160        170        180 
NNSSQSQEEL FREFEDILVT FFKQIDSELG LDSPYDSFCS GTTAVTVFKQ ADCLVIANLG 

       190        200        210        220        230        240 
HSRAVLGTRS KNSFKAVQLT VDLKPCVQRE AERIVSCKGR VFAMEEEPDV YRVWMPDDDC 

       250        260        270        280        290        300 
PGLAMSRAFG DFCLKDYGLV CIPDVFCRKV SREDEFVVLA TDGIWDVLSN EEVVKVVGSC 

       310        320        330        340        350        360 
KDRSVAAEML VQRAARTWRT KFPASKADDC AVVVLYLNHR PYPREGNVSR AISTISWRSN 

       370        380 
KSNNECYGAA PLSPLGLSQR VS 

« Hide

Isoform 2 [UniParc].

Checksum: 497A7EE74B9A0F6D
Show »

FASTA33337,045

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL161946 Genomic DNA. Translation: CAB82286.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED90378.1.
CP002688 Genomic DNA. Translation: AED90379.1.
BX831823 mRNA. No translation available.
BT011618 mRNA. Translation: AAS47624.1.
BT012622 mRNA. Translation: AAT06441.1.
PIRT48191.
RefSeqNP_001031819.1. NM_001036742.1.
NP_195790.4. NM_120248.4.
UniGeneAt.33516.

3D structure databases

ProteinModelPortalQ6NKS1.
SMRQ6NKS1. Positions 43-340.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ6NKS1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G01700.2; AT5G01700.2; AT5G01700. [Q6NKS1-1]
GeneID831695.
KEGGath:AT5G01700.

Organism-specific databases

TAIRAT5G01700.

Phylogenomic databases

eggNOGCOG0631.
HOGENOMHOG000233897.
InParanoidQ6NKS1.
OMAISTISWR.
PhylomeDBQ6NKS1.
ProtClustDBCLSN2681021.

Enzyme and pathway databases

BioCycARA:AT5G01700-MONOMER.
ARA:GQT-1503-MONOMER.

Gene expression databases

GenevestigatorQ6NKS1.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
ProtoNetSearch...

Entry information

Entry nameP2C65_ARATH
AccessionPrimary (citable) accession number: Q6NKS1
Secondary accession number(s): Q2V3A9, Q9M006
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names