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Protein

Probable protein phosphatase 2C 65

Gene

At5g01700

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi83 – 831Manganese 1By similarity
Metal bindingi83 – 831Manganese 2By similarity
Metal bindingi84 – 841Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi282 – 2821Manganese 2By similarity
Metal bindingi328 – 3281Manganese 2By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G01700-MONOMER.
ARA:GQT-1503-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 65 (EC:3.1.3.16)
Short name:
AtPP2C65
Gene namesi
Ordered Locus Names:At5g01700
ORF Names:F7A7.220
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G01700.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Probable protein phosphatase 2C 65PRO_0000367987Add
BLAST

Proteomic databases

PRIDEiQ6NKS1.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G01700.2.

Structurei

3D structure databases

ProteinModelPortaliQ6NKS1.
SMRiQ6NKS1. Positions 43-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 337291PPM-type phosphatasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233897.
InParanoidiQ6NKS1.
OMAiISTISWR.
PhylomeDBiQ6NKS1.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS51746. PPM_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6NKS1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVCCSKGTG IIVEHGADDG NECGDGEAEV RDTNDGAVVR TRGSSKHVSM
60 70 80 90 100
SIKQGKKGIN QDAMTVWENF GGEEDTIFCG VFDGHGPMGH KISRHVCENL
110 120 130 140 150
PSRVHSKIRS SKSAGDENIE NNSSQSQEEL FREFEDILVT FFKQIDSELG
160 170 180 190 200
LDSPYDSFCS GTTAVTVFKQ ADCLVIANLG HSRAVLGTRS KNSFKAVQLT
210 220 230 240 250
VDLKPCVQRE AERIVSCKGR VFAMEEEPDV YRVWMPDDDC PGLAMSRAFG
260 270 280 290 300
DFCLKDYGLV CIPDVFCRKV SREDEFVVLA TDGIWDVLSN EEVVKVVGSC
310 320 330 340 350
KDRSVAAEML VQRAARTWRT KFPASKADDC AVVVLYLNHR PYPREGNVSR
360 370 380
AISTISWRSN KSNNECYGAA PLSPLGLSQR VS
Length:382
Mass (Da):42,004
Last modified:July 5, 2004 - v1
Checksum:i27DA3A16DE5E77CA
GO
Isoform 2 (identifier: Q6NKS1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Note: No experimental confirmation available.
Show »
Length:333
Mass (Da):37,045
Checksum:i497A7EE74B9A0F6D
GO

Sequence cautioni

The sequence BX831823 differs from that shown.Sequencing errors.Curated
The sequence CAB82286.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4949Missing in isoform 2. 1 PublicationVSP_036776Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161946 Genomic DNA. Translation: CAB82286.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED90378.1.
CP002688 Genomic DNA. Translation: AED90379.1.
BX831823 mRNA. No translation available.
BT011618 mRNA. Translation: AAS47624.1.
BT012622 mRNA. Translation: AAT06441.1.
PIRiT48191.
RefSeqiNP_001031819.1. NM_001036742.1. [Q6NKS1-1]
NP_195790.4. NM_120248.4. [Q6NKS1-2]
UniGeneiAt.33516.

Genome annotation databases

EnsemblPlantsiAT5G01700.2; AT5G01700.2; AT5G01700. [Q6NKS1-1]
GeneIDi831695.
KEGGiath:AT5G01700.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL161946 Genomic DNA. Translation: CAB82286.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED90378.1.
CP002688 Genomic DNA. Translation: AED90379.1.
BX831823 mRNA. No translation available.
BT011618 mRNA. Translation: AAS47624.1.
BT012622 mRNA. Translation: AAT06441.1.
PIRiT48191.
RefSeqiNP_001031819.1. NM_001036742.1. [Q6NKS1-1]
NP_195790.4. NM_120248.4. [Q6NKS1-2]
UniGeneiAt.33516.

3D structure databases

ProteinModelPortaliQ6NKS1.
SMRiQ6NKS1. Positions 43-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G01700.2.

Proteomic databases

PRIDEiQ6NKS1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G01700.2; AT5G01700.2; AT5G01700. [Q6NKS1-1]
GeneIDi831695.
KEGGiath:AT5G01700.

Organism-specific databases

TAIRiAT5G01700.

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233897.
InParanoidiQ6NKS1.
OMAiISTISWR.
PhylomeDBiQ6NKS1.

Enzyme and pathway databases

BioCyciARA:AT5G01700-MONOMER.
ARA:GQT-1503-MONOMER.

Miscellaneous databases

PROiQ6NKS1.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C65_ARATH
AccessioniPrimary (citable) accession number: Q6NKS1
Secondary accession number(s): Q2V3A9, Q9M006
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 5, 2004
Last modified: June 24, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.