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Q6NKC7

- BIOB1_CORDI

UniProt

Q6NKC7 - BIOB1_CORDI

Protein

Biotin synthase 1

Gene

bioB1

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi90 – 901Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi93 – 931Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi129 – 1291Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi221 – 2211Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi291 – 2911Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthase 1UniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioB1UniRule annotation
    Ordered Locus Names:DIP0105
    OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
    Taxonomic identifieri257309 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000002198: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 350350Biotin synthase 1PRO_0000381325Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi257309.DIP0105.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6NKC7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiMIIARIF.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6NKC7-1 [UniParc]FASTAAdd to Basket

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    MIIARIFGHL PIATTWEHHV TDILELARTK VLNNGEGLNK EEVLQVLQLD    50
    EARIPELLEL AHEVRLKWCG EEVEVEGIIS LKTGGCPEDC HFCSQSGLFE 100
    SPVRSAWLDI AGLVEAAKQT QKSGATEFCI VAAVKGPDER LMSQLEEAVA 150
    AIKSEVDIEV AASIGILTQE QVDRLKAAGV HRYNHNLETA RSYFPNVVTT 200
    HSWESRRETL RMVGEAGMEV CSGGIIGMGE TLEQRAEFAC DLAELNPTEV 250
    PMNFLDPRPG TPFADYEVLD TTDALRAIGA FRLALPKTIL RFAGGRELTL 300
    GDLGTEQGLL GGINAVIVGN YLTTLGRPME QDLDMLGKLR LPIKALNASV 350
    Length:350
    Mass (Da):38,224
    Last modified:July 5, 2004 - v1
    Checksum:iE54D971CF77C1D1A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248354 Genomic DNA. Translation: CAE48610.1.
    RefSeqiNP_938502.1. NC_002935.2.
    WP_010934019.1. NC_002935.2.

    Genome annotation databases

    EnsemblBacteriaiCAE48610; CAE48610; DIP0105.
    GeneIDi2649072.
    KEGGicdi:DIP0105.
    PATRICi21481367. VBICorDip47633_0097.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248354 Genomic DNA. Translation: CAE48610.1 .
    RefSeqi NP_938502.1. NC_002935.2.
    WP_010934019.1. NC_002935.2.

    3D structure databases

    ProteinModelPortali Q6NKC7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257309.DIP0105.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE48610 ; CAE48610 ; DIP0105 .
    GeneIDi 2649072.
    KEGGi cdi:DIP0105.
    PATRICi 21481367. VBICorDip47633_0097.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi MIIARIF.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

    Entry informationi

    Entry nameiBIOB1_CORDI
    AccessioniPrimary (citable) accession number: Q6NKC7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3