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Q6NKC7 (BIOB1_CORDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase 1

EC=2.8.1.6
Gene names
Name:bioB1
Ordered Locus Names:DIP0105
OrganismCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis) [Complete proteome] [HAMAP]
Taxonomic identifier257309 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 350350Biotin synthase 1 HAMAP-Rule MF_01694
PRO_0000381325

Sites

Metal binding861Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding901Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding931Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1291Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2211Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2911Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6NKC7 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: E54D971CF77C1D1A

FASTA35038,224
        10         20         30         40         50         60 
MIIARIFGHL PIATTWEHHV TDILELARTK VLNNGEGLNK EEVLQVLQLD EARIPELLEL 

        70         80         90        100        110        120 
AHEVRLKWCG EEVEVEGIIS LKTGGCPEDC HFCSQSGLFE SPVRSAWLDI AGLVEAAKQT 

       130        140        150        160        170        180 
QKSGATEFCI VAAVKGPDER LMSQLEEAVA AIKSEVDIEV AASIGILTQE QVDRLKAAGV 

       190        200        210        220        230        240 
HRYNHNLETA RSYFPNVVTT HSWESRRETL RMVGEAGMEV CSGGIIGMGE TLEQRAEFAC 

       250        260        270        280        290        300 
DLAELNPTEV PMNFLDPRPG TPFADYEVLD TTDALRAIGA FRLALPKTIL RFAGGRELTL 

       310        320        330        340        350 
GDLGTEQGLL GGINAVIVGN YLTTLGRPME QDLDMLGKLR LPIKALNASV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248354 Genomic DNA. Translation: CAE48610.1.
RefSeqNP_938502.1. NC_002935.2.

3D structure databases

ProteinModelPortalQ6NKC7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257309.DIP0105.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE48610; CAE48610; DIP0105.
GeneID2649072.
KEGGcdi:DIP0105.
PATRIC21481367. VBICorDip47633_0097.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMAMIIARIF.
OrthoDBEOG622PMP.

Enzyme and pathway databases

UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB1_CORDI
AccessionPrimary (citable) accession number: Q6NKC7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways