ID Q6NK15_CORDI Unreviewed; 560 AA. AC Q6NK15; DT 05-JUL-2004, integrated into UniProtKB/TrEMBL. DT 05-JUL-2004, sequence version 1. DT 27-MAR-2024, entry version 96. DE SubName: Full=Diphtheria toxin {ECO:0000313|EMBL:CAE48728.1}; DE EC=2.4.2.36 {ECO:0000313|EMBL:CAE48728.1}; GN Name=tox {ECO:0000313|EMBL:CAE48728.1}; GN OrderedLocusNames=DIP0222 {ECO:0000313|EMBL:CAE48728.1}; OS Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype OS gravis). OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; OC Corynebacteriaceae; Corynebacterium. OX NCBI_TaxID=257309 {ECO:0000313|EMBL:CAE48728.1, ECO:0000313|Proteomes:UP000002198}; RN [1] {ECO:0000313|EMBL:CAE48728.1, ECO:0000313|Proteomes:UP000002198} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 700971 / NCTC 13129 / Biotype gravis RC {ECO:0000313|Proteomes:UP000002198}; RX PubMed=14602910; DOI=10.1093/nar/gkg874; RA Cerdeno-Tarraga A.M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M., RA Bentley S.D., Besra G.S., Churcher C., James K.D., De Zoysa A., RA Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., RA Jagels K., Moule S., Quail M.A., Rabbinowitsch E., Rutherford K., RA Thomson N.R., Unwin L., Whitehead S., Barrell B.G.Parkhill.J.; RT "The complete genome sequence and analysis of Corynebacterium diphtheriae RT NCTC13129."; RL Nucleic Acids Res. 31:6516-6523(2003). RN [2] {ECO:0007829|PDB:5I82} RP X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 26-560, AND DISULFIDE BONDS. RX PubMed=29875175; DOI=10.1042/bsr20180238; RA Mishra R.P.N., Yadav R.S.P., Jones C., Nocadello S., Minasov G., RA Shuvalova L.A., Anderson W.F., Goel A.; RT "Structural and immunological characterization of E. coli derived RT recombinant CRM197 protein used as carrier in conjugate vaccines. ."; RL Biosci. Rep. 38:BSR20180238-BSR20180238(2018). RN [3] {ECO:0007829|PDB:7K7B, ECO:0007829|PDB:7K7C} RP X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 26-560, AND DISULFIDE BONDS. RX PubMed=33171806; DOI=10.3390/toxins12110704; RA Rodnin M.V., Kashipathy M.M., Kyrychenko A., Battaile K.P., Lovell S., RA Ladokhin A.S.; RT "Structure of the Diphtheria Toxin at Acidic pH: Implications for the RT Conformational Switching of the Translocation Domain. ."; RL Toxins 12:E704-E704(2020). RN [4] {ECO:0007829|PDB:7O4W} RP X-RAY CRYSTALLOGRAPHY (2.03 ANGSTROMS) OF 26-560, AND DISULFIDE BONDS. RX PubMed=35310500; DOI=10.1039/d1sc01928g; RA Carboni F., Kitowski A., Sorieul C., Veggi D., Marques M.C., Oldrini D., RA Balducci E., Brogioni B., Del Bino L., Corrado A., Angiolini F., RA Dello Iacono L., Margarit I., Romano M.R., Bernardes G.J.L., Adamo R.; RT "Retaining the structural integrity of disulfide bonds in diphtheria toxoid RT carrier protein is crucial for the effectiveness of glycoconjugate vaccine RT candidates."; RL Chem. Sci. 13:2440-2449(2022). RN [5] {ECO:0007829|PDB:7RRW} RP X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 26-560, AND DISULFIDE BONDS. RX PubMed=36995122; DOI=10.1107/s2053230x23002364; RA Gallagher D.T., Oganesyan N., Lees A.; RT "Monomeric crystal structure of the vaccine carrier protein RT CRM197 and implications for vaccine development."; RL Acta Crystallogr. F Struct. Biol. Commun. 79:82-86(2023). RN [6] {ECO:0007829|PDB:8G0F} RP X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 26-560, AND DISULFIDE BONDS. RX PubMed=37505680; DOI=10.3390/toxins15070410; RA Rodnin M.V., Vasques-Montes V., Kyrychenko A., Oliveira N.F.B., RA Kashipathy M.M., Battaile K.P., Douglas J., Lovell S., Machuqueiro M., RA Ladokhin A.S.; RT "Histidine Protonation and Conformational Switching in Diphtheria Toxin RT Translocation Domain."; RL Toxins 15:410-410(2023). CC -!- INTERACTION: CC Q6NK15; Q6NK15: tox; NbExp=2; IntAct=EBI-7700183, EBI-7700183; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BX248354; CAE48728.1; -; Genomic_DNA. DR RefSeq; WP_003850266.1; NC_002935.2. DR PDB; 5I82; X-ray; 2.35 A; A/B/C/D=26-560. DR PDB; 7K7B; X-ray; 2.05 A; A/B=26-560. DR PDB; 7K7C; X-ray; 2.05 A; A/B=26-560. DR PDB; 7K7D; X-ray; 2.10 A; A/B=26-560. DR PDB; 7K7E; X-ray; 2.30 A; A/B=26-560. DR PDB; 7O4W; X-ray; 2.03 A; A=26-560. DR PDB; 7RRW; X-ray; 2.00 A; A=26-560. DR PDB; 8G0F; X-ray; 2.25 A; A/B=26-560. DR PDBsum; 5I82; -. DR AlphaFoldDB; Q6NK15; -. DR SMR; Q6NK15; -. DR MINT; Q6NK15; -. DR STRING; 257309.DIP0222; -. DR KEGG; cdi:DIP0222; -. DR HOGENOM; CLU_486373_0_0_11; -. DR PHI-base; PHI:11205; -. DR Proteomes; UP000002198; Chromosome. DR GO; GO:0005615; C:extracellular space; IEA:InterPro. DR GO; GO:0042802; F:identical protein binding; IPI:IntAct. DR GO; GO:0047286; F:NAD+-diphthamide ADP-ribosyltransferase activity; IEA:UniProtKB-EC. DR GO; GO:0090729; F:toxin activity; IEA:InterPro. DR Gene3D; 3.90.175.10; Diphtheria Toxin, domain 1; 1. DR Gene3D; 2.60.40.700; Diphtheria toxin, receptor-binding domain; 1. DR Gene3D; 1.10.490.40; Diphtheria toxin, translocation domain; 1. DR InterPro; IPR036799; Diphtheria_tox_rcpt-bd_dom_sf. DR InterPro; IPR036801; Diphtheria_tox_transloc_sf. DR InterPro; IPR000512; Diphtheria_toxin. DR InterPro; IPR022406; Diphtheria_toxin_catalytic_dom. DR InterPro; IPR022404; Diphtheria_toxin_rcpt-bd_dom. DR Pfam; PF02763; Diphtheria_C; 1. DR Pfam; PF01324; Diphtheria_R; 1. DR Pfam; PF02764; Diphtheria_T; 1. DR PIRSF; PIRSF000490; Diphtheria_toxin; 1. DR PRINTS; PR00769; DPTHRIATOXIN. DR SUPFAM; SSF56399; ADP-ribosylation; 1. DR SUPFAM; SSF49380; Diphtheria toxin, C-terminal domain; 1. DR SUPFAM; SSF56845; Diphtheria toxin, middle domain; 1. PE 1: Evidence at protein level; KW 3D-structure {ECO:0007829|PDB:5I82, ECO:0007829|PDB:7K7B}; KW Glycosyltransferase {ECO:0000313|EMBL:CAE48728.1}; KW Reference proteome {ECO:0000313|Proteomes:UP000002198}; KW Signal {ECO:0000256|SAM:SignalP}; KW Transferase {ECO:0000313|EMBL:CAE48728.1}. FT SIGNAL 1..25 FT /evidence="ECO:0000256|SAM:SignalP" FT CHAIN 26..560 FT /evidence="ECO:0000256|SAM:SignalP" FT /id="PRO_5004279123" FT DOMAIN 26..212 FT /note="Diphtheria toxin catalytic" FT /evidence="ECO:0000259|Pfam:PF02763" FT DOMAIN 406..559 FT /note="Diphtheria toxin receptor-binding" FT /evidence="ECO:0000259|Pfam:PF01324" FT DISULFID 211..226 FT /evidence="ECO:0007829|PDB:5I82, ECO:0007829|PDB:7K7B" FT DISULFID 486..496 FT /evidence="ECO:0007829|PDB:5I82, ECO:0007829|PDB:7K7B" SQ SEQUENCE 560 AA; 60815 MW; 51F5718361B94EAB CRC64; MSRKLFASIL IGALLGIGAP PSAHAGADDV VDSSKSFVME NFSSYHGTKP GYVDSIQKGI QKPKSGTQGN YDDDWKGFYS TDNKYDAAGY SVDNENPLSG KAGGVVKVTY PGLTKVLALK VDNAETIKKE LGLSLTEPLM EQVGTEEFIK RFGDGASRVV LSLPFAEGSS SVEYINNWEQ AKALSVELEI NFETRGKRGQ DAMYEYMAQA CAGNRVRRSV GSSLSCINLD WDVIRDKTKT KIESLKEHGP IKNKMSESPN KTVSEEKAKQ YLEEFHQTAL EHPELSELKT VTGTNPVFAG ANYAAWAVNV AQVIDSETAD NLEKTTAALS ILPGIGSVMG IADGAVHHNT EEIVAQSIAL SSLMVAQAIP LVGELVDIGF AAYNFVESII NLFQVVHNSY NRPAYSPGHK TQPFLHDGYA VSWNTVEDSI IRTGFQGESG HDIKITAENT PLPIAGVLLP TIPGKLDVNK SKTHISVNGR KIRMRCRAID GDVTFCRPKS PVYVGNGVHA NLHVAFHRSS SEKIHSNEIS SDSIGVLGYQ KTVDHTKVNS KLSLFFEIKS //