Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q6NJR6 (Q6NJR6_CORDI) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Name:topA EMBL CAE48833.1
Ordered Locus Names:DIP0327
OrganismCorynebacterium diphtheriae [Complete proteome] [HAMAP]
Taxonomic identifier1717 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length964 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
Q6NJR6 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 3136B27538E327F5

FASTA964106,551
        10         20         30         40         50         60 
MAAKTGNKRL VIVESATKAK KIAPYLGDDY IVEASVGHIR DLPRGAADVP AKYKKEPWAR 

        70         80         90        100        110        120 
LGVNTDKGFE PLYVVSPDKK KKVADLKAKL KLVDELFLAT DPDREGEAIA WHLLEVLKPK 

       130        140        150        160        170        180 
VPVKRMVFNE ITKPAILAAA ENTRELDDNL VDAQETRRIL DRLYGYEVSP VLWKKVMPRL 

       190        200        210        220        230        240 
SAGRVQSVAT RVIVERERER MAFISADYWD VEAEFDTGKP AQDSNPTSFT GRLTALDGKR 

       250        260        270        280        290        300 
VATGRDFNDR GELKGDAVVV DKQRAEALVE ELTGVAMSVT KVEEKPYTRR PYAPFMTSTL 

       310        320        330        340        350        360 
QQEAGRKLHY TSERTMRIAQ RLYENGHITY MRTDSTTLSE QGLKAAREQA ISLYGADYVA 

       370        380        390        400        410        420 
DAPRRYDRKV KNSQEAHEAI RPAGERFATP GDLHGQLDAE EFKLYELIWQ RTVASQMADA 

       430        440        450        460        470        480 
KGTSMKVTIT GNNAEFSATG RTITFPGFLR AYVEVSTLAD GRDVADNAES HLPRLAEGDG 

       490        500        510        520        530        540 
VETKNLEADG HSTNPPARYT EASLVKKMED LGIGRPSTYA SIIKTIQDRG YVYSRGNALV 

       550        560        570        580        590        600 
PSWVAFAVIG LLEKSFAALV DYDFTSSMED ELDEIAAGHE NGAHWLTGFY FGDAEASDAK 

       610        620        630        640        650        660 
AESIARHGGL KALVGDNLEQ IDARLVNSLP LFKDQEGRDV NVRVGRYGPY LERQVGVSED 

       670        680        690        700        710        720 
GEPEYQRANL SDSTTPDELT LELAEKLFAT PQSGRELGRN PKNNRMIVAK EGRFGPYVTE 

       730        740        750        760        770        780 
LVNDDERTEV EAKAEEIVAA EREAEDLQRA AEGKRAKNWG TKTAAAQKEK RIAQIIEETL 

       790        800        810        820        830        840 
KPSTASLFKT MEPSSVTLDE ALQLLALPRE VGVDPSDNEV ITAQNGRYGP YLKKGSDSRT 

       850        860        870        880        890        900 
LASEDQIFSI TLDEARRIYA EPKRRGRGAT SQSVIKQLGD NDVSGKPMSV RDGRFGPYVT 

       910        920        930        940        950        960 
DGTTNASLRR GDDPTQLTEA RANELLSERR AKEAADGGSS KKATKKASKK TTKRVAKAGA 


RKKK

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX248354 Genomic DNA. Translation: CAE48833.1.
RefSeqNP_938715.1. NC_002935.2.

3D structure databases

ProteinModelPortalQ6NJR6.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2649678.
GenomeReviewsGene locus DIP0327 in contig BX248353_GR.
KEGGcdi:DIP0327.
NMPDRfig|257309.1.peg.306.
PATRIC21481813. VBICorDip47633_0312.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG512935.
OMAKKVMPRL.
PhylomeDBQ6NJR6.
ProtClustDBPRK07561.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ6NJR6_CORDI
AccessionPrimary (citable) accession number: Q6NJR6
Entry history
Integrated into UniProtKB/TrEMBL: July 5, 2004
Last sequence update: July 5, 2004
Last modified: December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info