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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 1211D-inositol 3-phosphateUniRule annotation
Binding sitei26 – 261UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei81 – 8111D-inositol 3-phosphateUniRule annotation
Binding sitei113 – 11311D-inositol 3-phosphateUniRule annotation
Binding sitei137 – 13711D-inositol 3-phosphateUniRule annotation
Binding sitei157 – 15711D-inositol 3-phosphateUniRule annotation
Binding sitei234 – 2341UDP-GlcNAcUniRule annotation
Binding sitei239 – 2391UDP-GlcNAcUniRule annotation
Binding sitei297 – 2971UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi306 – 3061Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi307 – 3071Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi309 – 3091Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei319 – 3191UDP-GlcNAcUniRule annotation
Binding sitei327 – 3271UDP-GlcNAcUniRule annotation
Metal bindingi333 – 3331MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:DIP0388
OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Taxonomic identifieri257309 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000002198 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427D-inositol 3-phosphate glycosyltransferasePRO_0000400116Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ6NJL3.
SMRiQ6NJL3. Positions 4-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni18 – 192UDP-GlcNAc bindingUniRule annotation
Regioni23 – 2861D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6NJL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMTMRIAMIS IHTSPLQQPG SGDAGGMNVY IISIARELAR RGVDVDIYTR
60 70 80 90 100
ATRPSQGDVV EVESGLRVIN IVAGPYEGLS KEELPTQLAA FAGGVVQFAK
110 120 130 140 150
CHHMRYDVIH SHYWLSGQVG WLLRDLWNIP LVHTAHTLAA VKNAHRSAGD
160 170 180 190 200
TEESEARRIC EQQLVDNADI LVVNTPEETN DLVRHYDANP DSVAVIAPGA
210 220 230 240 250
NVELFTPGTQ RNTEQSRRCL GIPLHTKVMA FVGRLQQFKG PEVLLRAVAE
260 270 280 290 300
MLERDPDRDM RVIMCGGPSG AAATVEHYIE LTRSLGIAHR VRFLDPRPPE
310 320 330 340 350
ELVSVYQAAD VVAVPSYNES FGLVAMEAQA SGTPVVAARV GGLPIAVVDG
360 370 380 390 400
ETGVLVDGHD PIMWADALEQ LLDDDPTRQQ MGVAAVEHAA NFTWAAAAEK
410 420
LESVYGDAAM LDVAQCHDRY AAGSDRA
Length:427
Mass (Da):46,352
Last modified:July 5, 2004 - v1
Checksum:i4E101F9D30B9630F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248355 Genomic DNA. Translation: CAE48892.1.
RefSeqiWP_010934263.1. NC_002935.2.

Genome annotation databases

EnsemblBacteriaiCAE48892; CAE48892; DIP0388.
GeneIDi2650670.
KEGGicdi:DIP0388.
PATRICi21481931. VBICorDip47633_0370.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248355 Genomic DNA. Translation: CAE48892.1.
RefSeqiWP_010934263.1. NC_002935.2.

3D structure databases

ProteinModelPortaliQ6NJL3.
SMRiQ6NJL3. Positions 4-408.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

DNASUi2650670.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE48892; CAE48892; DIP0388.
GeneIDi2650670.
KEGGicdi:DIP0388.
PATRICi21481931. VBICorDip47633_0370.

Phylogenomic databases

HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_CORDI
AccessioniPrimary (citable) accession number: Q6NJL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.