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Q6NJH8 (MEND_CORDI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Short name=SEPHCHC synthase
EC=2.2.1.9
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene names
Name:menD
Ordered Locus Names:DIP0423
OrganismCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis) [Complete proteome] [HAMAP]
Taxonomic identifier257309 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity. HAMAP-Rule MF_01659

Catalytic activity

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2. HAMAP-Rule MF_01659

Cofactor

Magnesium or manganese By similarity. HAMAP-Rule MF_01659

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 2/8. HAMAP-Rule MF_01659

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01659

Sequence similarities

Belongs to the TPP enzyme family. MenD subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5095092-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase HAMAP-Rule MF_01659
PRO_0000341726

Sequences

Sequence LengthMass (Da)Tools
Q6NJH8 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: BDD2B2DD303A2068

FASTA50954,051
        10         20         30         40         50         60 
MTSSPELARA VAATLARHVT DVVICPGSRN SPLSLELIAR DDIRVHTRID ERSAAFLALG 

        70         80         90        100        110        120 
LARSSKRHVA VVTTSGTAVA NCAPAMIEAA YSHTPLIMVS ADRPERLHGT GANQTIEQRG 

       130        140        150        160        170        180 
IFNVVDTDHV VDITDLNSIS FARNVIHINV ALDVPLVDVL PEVGDPVERV REVIEVDHGE 

       190        200        210        220        230        240 
VVLNLNERIL VVAGDEAWEV PGLEDVPTIA EPSAPAPYHP VHPLAAQIFA HKEIKPEHIV 

       250        260        270        280        290        300 
VVGHPTLHRA VLSLLADVPV TVLTKTDTIT GNPQAVGSRV KVTGELDKRW LQICEDASTL 

       310        320        330        340        350        360 
CADRVKEVLE GHGFTGLHVA AAVADTMAVG DTLFLGASNP VRDASLVGMP LGGVDIFAAR 

       370        380        390        400        410        420 
GAAGIDGTVS QAVGVALDVQ HRDATAIRAD RTVALMGDVT FLHDVGGLLG ARPENLTIVV 

       430        440        450        460        470        480 
ANDDGCGIFE TLEIGAPEFR PSFEQAFGTP HGVHIEAIAQ AYGVNYLRAE TLPELIEALI 

       490        500 
DTTDSAGFNI IEAVTTRSTR RDIDKALTL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248355 Genomic DNA. Translation: CAE48927.1.
RefSeqNP_938804.1. NC_002935.2.

3D structure databases

ProteinModelPortalQ6NJH8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257309.DIP0423.

Protocols and materials databases

DNASU2649096.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE48927; CAE48927; DIP0423.
GeneID2649096.
KEGGcdi:DIP0423.
PATRIC21482003. VBICorDip47633_0406.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1165.
HOGENOMHOG000218359.
KOK02551.
OMAWRSAVCR.
OrthoDBEOG6NWBQW.

Enzyme and pathway databases

UniPathwayUPA00079; UER00164.

Family and domain databases

Gene3D3.40.50.970. 2 hits.
HAMAPMF_01659. MenD.
InterProIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamPF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFPIRSF004983. MenD. 1 hit.
SUPFAMSSF52518. SSF52518. 2 hits.
TIGRFAMsTIGR00173. menD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMEND_CORDI
AccessionPrimary (citable) accession number: Q6NJH8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways