Q6NJ04 (DNAE2_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase
EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	DIP0612

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	1039 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1039	1039	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103376

Sequences

	Sequence		Length	Mass (Da)
	Q6NJ04 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B0978740FB847B14	FASTA	1,039	113,416
	10 20 30 40 50 60 MVDWVSGVNG KPLKWSQLEG VLSGTYPQEF KLANVGGSGS GSEKIRYYSS QPFAELHAAS 70 80 90 100 110 120 YYHFLHGANS PQDMVAAACQ QGMSALALID RDGFYGAMEF AEAAATSGLP TVFGAELTLA 130 140 150 160 170 180 PDRILTVLAR GVEGYTSLSR LITQARMRGS KDVTIYPPLA EIVESMGKNC RFLLNHSWCT 190 200 210 220 230 240 DESLLEIFYP DTVVLEYGVT MRPEDVDHHE LLDSIRQRYG FMAIASAVPS AVDRVSARVA 250 260 270 280 290 300 GAKQALAQRE SLPQATPRLH PMGSTWMRGG DELAGSVDKQ LLDASVEVAR DCAFALDLVA 310 320 330 340 350 360 PNLPRWPVPD EMAHLTGLVN ARFDARYRGR SEDVKNQARA QIHHELAVIK ELGFPGYFLI 370 380 390 400 410 420 VDDIVSFCHT NNILCQGRGS AANSVVCFVL GITNVEPIST GLLFERFLSR DREGPPDIDI 430 440 450 460 470 480 DIESGRRDAV ITYVYDTYGR DNAAQVANVI TYRTKAALRD AARALGYPNT SADAWSTNPD 490 500 510 520 530 540 EAPNAVRFLA EQLKGQPRHL GIHAGGMVIC DRPIADVVPT EWARKHGRSV VQWDKESCAS 550 560 570 580 590 600 AGLVKFDLLG LGMLEALHHM IDLVVETTGT TINLWEIDLA EPEIYTMLSN GDAVGVFQVE 610 620 630 640 650 660 SRAQLATLPR LRPREFFDLV VEVALVRPGP IQGGSVHPYI RRRNGLEAVT YEHPILERSL 670 680 690 700 710 720 RKTLGVPLFQ EQLMHIAVDA AGFSGGEADE LRRAMGSRRS VDTMNRMKRR FFAGLRRNNI 730 740 750 760 770 780 TGDIAGILWN KIVAFVAYGF PESHSQSFAS LVYFSAWFKY HHPAEFYVGL LRAQPMGFYS 790 800 810 820 830 840 PQSLLADARR KELTILSHNI NKSQVEATVE NGAIRLGLNM IKGVGKTEAE AIVAHAPYRD 850 860 870 880 890 900 IADLSRRAGL GVAVIEALAR SGALETLGVG RRQALWQAGA AATEKAAMLP GLSMVSAPSL 910 920 930 940 950 960 PGMNAFELIV ADIAMTGVSS ENPVALLRRQ LELRGVIPAS ELLNCENARR ILLAGVVTHR 970 980 990 1000 1010 1020 QRPQTAAGVT FLGLEDETGL ANVVVSVGLW KRSRAVKVAR AVLVRGVVHN ADGVASVTAD 1030 RVEELPFAEM MSAGSRDFR

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248355 Genomic DNA. Translation: CAE49129.1.
RefSeq	NP_938986.1. NC_002935.2.
3D structure databases
ProteinModelPortal	Q6NJ04.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2648530.
GenomeReviews	Gene locus DIP0612 in contig BX248353_GR.
KEGG	cdi:DIP0612.
NMPDR	fig|257309.1.peg.577.
PATRIC	21482395. VBICorDip47633_0598.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734490.
OMA	ERIGNSD.
ProtClustDB	PRK05672.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP0612-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. [Graphical view]
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF89550. PHP-like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_CORDI

Accession

Primary (citable) accession number: Q6NJ04

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program