Q6NIF2 (TYSY_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	DIP0825

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Thymidylate synthase HAMAP MF_00008		PRO_0000140950
Sites
	Active site	153	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NIF2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 53D11631FB668A9E	FASTA	270	30,835
	10 20 30 40 50 60 MSTNNTIKTP YEDLLRTILE QGSHKDDRTG TGTTSLFGQQ MRFDLTEAFP LITTKKVYWK 70 80 90 100 110 120 GVIGELLWFL QGSSNVRWLQ KRNIHIWDEW ASEEGELGPV YGVQWRSWPT PDGQHVDQIA 130 140 150 160 170 180 QALDILKNNP DSRRNIVSAW NVADLNNMAL PPCHLLFQLY VADGKLSCQL YQRSADMFLG 190 200 210 220 230 240 VPFNIASYSA LTHMLAQQAG LKVGEFIWTG GDCHIYDNHR EQVLTQLSRE PRPYPQLTLR 250 260 270 KASSIFDYDF DDFTIEGYDP HPAIRGEVAV

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248356 Genomic DNA. Translation: CAE49341.1.
RefSeq	NP_939189.1. NC_002935.2.
3D structure databases
ProteinModelPortal	Q6NIF2.
SMR	Q6NIF2. Positions 11-270.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2650540.
GenomeReviews	Gene locus DIP0825 in contig BX248353_GR.
KEGG	cdi:DIP0825.
NMPDR	fig|257309.1.peg.780.
PATRIC	21482819. VBICorDip47633_0805.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG588098.
OMA	RMALAPC.
PhylomeDB	Q6NIF2.
ProtClustDB	PRK01827.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP0825-MONOMER.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_CORDI

Accession

Primary (citable) accession number: Q6NIF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program