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Q6NIF2 (TYSY_CORDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:DIP0825
OrganismCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis) [Complete proteome] [HAMAP]
Taxonomic identifier257309 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 270270Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000140950

Sites

Active site1531 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6NIF2 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 53D11631FB668A9E

FASTA27030,835
        10         20         30         40         50         60 
MSTNNTIKTP YEDLLRTILE QGSHKDDRTG TGTTSLFGQQ MRFDLTEAFP LITTKKVYWK 

        70         80         90        100        110        120 
GVIGELLWFL QGSSNVRWLQ KRNIHIWDEW ASEEGELGPV YGVQWRSWPT PDGQHVDQIA 

       130        140        150        160        170        180 
QALDILKNNP DSRRNIVSAW NVADLNNMAL PPCHLLFQLY VADGKLSCQL YQRSADMFLG 

       190        200        210        220        230        240 
VPFNIASYSA LTHMLAQQAG LKVGEFIWTG GDCHIYDNHR EQVLTQLSRE PRPYPQLTLR 

       250        260        270 
KASSIFDYDF DDFTIEGYDP HPAIRGEVAV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248356 Genomic DNA. Translation: CAE49341.1.
RefSeqNP_939189.1. NC_002935.2.

3D structure databases

ProteinModelPortalQ6NIF2.
SMRQ6NIF2. Positions 11-270.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257309.DIP0825.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE49341; CAE49341; DIP0825.
GeneID2650540.
KEGGcdi:DIP0825.
PATRIC21482819. VBICorDip47633_0805.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257899.
KOK00560.
OMATEIDAFT.
OrthoDBEOG6K6V53.

Enzyme and pathway databases

UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 2 hits.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_CORDI
AccessionPrimary (citable) accession number: Q6NIF2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways