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Protein

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Gene

ispE

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.UniRule annotation

Catalytic activityi

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 3 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei13 – 131UniRule annotation
Active sitei156 – 1561UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi114 – 12411ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00056; UER00094.

Names & Taxonomyi

Protein namesi
Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseUniRule annotation (EC:2.7.1.148UniRule annotation)
Short name:
CMKUniRule annotation
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinaseUniRule annotation
Gene namesi
Name:ispEUniRule annotation
Ordered Locus Names:DIP0876
OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Taxonomic identifieri257309 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000002198 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3113114-diphosphocytidyl-2-C-methyl-D-erythritol kinasePRO_0000189210Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ6NIA1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GHMP kinase family. IspE subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000019600.
KOiK00919.
OMAiGYHELNT.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00061. IspE. 1 hit.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamiPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF010376. IspE. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00154. ispE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6NIA1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSYAITAVA SSKVNLHLGV GNARDDGYHE LVTVFQSLSL HDTITVTPAA
60 70 80 90 100
DDMHDAEDQG IVATLSVSGD SAQAVPTDAT NLAWQAAEKM YQAHRRNGGA
110 120 130 140 150
PAKRVHIAID KGIPVAGGMA GGSADAAATL AAMAQYLGNT NTEQEILSIA
160 170 180 190 200
AELGSDVPFT YLGDTRLGTG RGEQLVPVLS RGTYHWALAF SAQGLSTPEV
210 220 230 240 250
FRKIDQMDRQ PHLDVTALNA ALITGDPHKV AAHLHNDLQA AALSLRPELR
260 270 280 290 300
TILEQGRAAG ALAGIVSGSG PTCAFLCEDA ETAQAVAAEL SVHYRTAVAT
310
GPVRGAHVKG H
Length:311
Mass (Da):32,338
Last modified:July 5, 2004 - v1
Checksum:i1504BAEF015F80B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248356 Genomic DNA. Translation: CAE49393.1.
RefSeqiWP_010934631.1. NC_002935.2.

Genome annotation databases

EnsemblBacteriaiCAE49393; CAE49393; DIP0876.
GeneIDi2648490.
KEGGicdi:DIP0876.
PATRICi21482925. VBICorDip47633_0858.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248356 Genomic DNA. Translation: CAE49393.1.
RefSeqiWP_010934631.1. NC_002935.2.

3D structure databases

ProteinModelPortaliQ6NIA1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE49393; CAE49393; DIP0876.
GeneIDi2648490.
KEGGicdi:DIP0876.
PATRICi21482925. VBICorDip47633_0858.

Phylogenomic databases

HOGENOMiHOG000019600.
KOiK00919.
OMAiGYHELNT.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00094.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00061. IspE. 1 hit.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamiPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF010376. IspE. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00154. ispE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiISPE_CORDI
AccessioniPrimary (citable) accession number: Q6NIA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.