Q6NHU3 (KHSE_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	DIP1037

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Homoserine kinase HAMAP MF_00384		PRO_0000156563
Regions
	Nucleotide binding	95 – 105	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6NHU3 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 3D9F2B5126111A3E	FASTA	308	32,455
	10 20 30 40 50 60 MAIELPVGKK VTVTVPASSA NLGPGFDTLG LALSLYDTVE VEVTDHGLEV EVFGEGQGEL 70 80 90 100 110 120 PLDGSHLVVK AIRAGLKAAD VQVPGLRVVC HNNIPQSRGL GSSAAAAVAG VAAANGLAGF 130 140 150 160 170 180 PLDDARVVQL SSAFEGHPDN AAASVLGNAV VSWTEIPVDG RTEPQFKAVT INVDSRIKAT 190 200 210 220 230 240 ALVPDFHAST EAVRRVLPSD VTHLDARFNV SRCAVMTVAL QHHPELLWEG TRDRLHQPYR 250 260 270 280 290 300 ADVLPVTAEW VNRLRNRGYA AYLSGAGPTI MVLHTEPVDE AVLNDAREAG LRVLSLDVAD AVSVKVDA

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248356 Genomic DNA. Translation: CAE49558.1.
RefSeq	NP_939399.1. NC_002935.2.
3D structure databases
ProteinModelPortal	Q6NHU3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2648622.
GenomeReviews	Gene locus DIP1037 in contig BX248353_GR.
KEGG	cdi:DIP1037.
NMPDR	fig|257309.1.peg.990.
PATRIC	21483253. VBICorDip47633_1019.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646290.
OMA	DRLHQPY.
PhylomeDB	Q6NHU3.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1037-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_CORDI

Accession

Primary (citable) accession number: Q6NHU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program