Reviewed, UniProtKB/Swiss-Prot Q6NHR6 (GLGB_CORDI)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	DIP1065

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 732	732	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000188697
Sites
	Active site	304	1	By similarity
	Active site	339	1	By similarity
	Active site	344	1	By similarity
	Active site	407	1	By similarity
	Active site	409	1	By similarity
	Active site	462	1	By similarity
	Active site	529	1	By similarity
	Active site	530	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NHR6-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6A734BCBFC3B0441	FASTA	732	82,603
	10 20 30 40 50 60 MVSPTQITPV HPDVLHALKI CQYHDPHGVY GWHEVDAERA VIRTRHIGAE RVETLLSDST 70 80 90 100 110 120 VVELAAVGDD IFEAIVDHDA SCDYRLRIHW QGGQVTEQAD AYHFLPTLGD LDLHLINEGR 130 140 150 160 170 180 HERLWEVLGA NPTTITTAMG DVEGTAFAVW APNASGVAVI GDFCGWNPSQ YPMRSLGSTG 190 200 210 220 230 240 IWELFIPNIG VGTVYKFAIH THEGHRLDKA DPMAKRAEVA PATGSIIASS SYTWNDDTWM 250 260 270 280 290 300 TNRAHTDHDN TAMSVYEVHL GSWSQGQNYE ELATNLVDYV KEMGYTHVEF LPVAEHPFGG 310 320 330 340 350 360 SWGYQVSGYY APTSRWGTPD QLRLLIDAFH QAGIGVIVDW VPAHFPKDAF ALGRFDGQAL 370 380 390 400 410 420 YEHPDWRRGE QKDWGTYVFD FGRNEVRNFL VANALYWLEE FHVDGLRVDA VASMLYLDYS 430 440 450 460 470 480 REPGEWLPNI YGGRENLEAV QFLQEMNATV HKSHPGVMTI AEESTSWPGV TSPTWEGGLG 490 500 510 520 530 540 FSMKWNMGWM NDTLEYFSHE PIHRMYHHND ITFSMVYAYS EKFVLPFSHD EVVHGKGSLW 550 560 570 580 590 600 TRMPGDAWNK AAGLRTLYAY MYAHPGKNLL FQGQEFGQVK EWSEERSLDW GDMDGWEGEY 610 620 630 640 650 660 HRGIRTLVQD LNALYKDSPA LYSQDNNPAG FSWTKSDDAA NNILSFVRYG ADGSKILAVF 670 680 690 700 710 720 NFGGADHPSY KLGVPEGGNW KCILNTDAGI YEGEDNYLDS DVMAWDTDWD GYQHSLTVHI 730 PAMSGQLYRW EA

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248357 Genomic DNA. Translation: CAE49588.1.
RefSeq	NP_939426.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2649325.
GenomeReviews	Gene locus DIP1065 in contig BX248353_GR.
KEGG	cdi:DIP1065.
NMPDR	fig|257309.1.peg.1017.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6NHR6.
OMA	PKTASIV.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1065-MON.
BRENDA	2.4.1.18. 20483.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_CORDI

Accession

Primary (citable) accession number: Q6NHR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)