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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathway: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei409 – 4091NucleophileUniRule annotation
Active sitei462 – 4621Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:DIP1065
OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Taxonomic identifieri257309 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
ProteomesiUP000002198 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7327321,4-alpha-glucan branching enzyme GlgBPRO_0000188697Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ6NHR6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDDADHNT.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 2 hits.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6NHR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSPTQITPV HPDVLHALKI CQYHDPHGVY GWHEVDAERA VIRTRHIGAE
60 70 80 90 100
RVETLLSDST VVELAAVGDD IFEAIVDHDA SCDYRLRIHW QGGQVTEQAD
110 120 130 140 150
AYHFLPTLGD LDLHLINEGR HERLWEVLGA NPTTITTAMG DVEGTAFAVW
160 170 180 190 200
APNASGVAVI GDFCGWNPSQ YPMRSLGSTG IWELFIPNIG VGTVYKFAIH
210 220 230 240 250
THEGHRLDKA DPMAKRAEVA PATGSIIASS SYTWNDDTWM TNRAHTDHDN
260 270 280 290 300
TAMSVYEVHL GSWSQGQNYE ELATNLVDYV KEMGYTHVEF LPVAEHPFGG
310 320 330 340 350
SWGYQVSGYY APTSRWGTPD QLRLLIDAFH QAGIGVIVDW VPAHFPKDAF
360 370 380 390 400
ALGRFDGQAL YEHPDWRRGE QKDWGTYVFD FGRNEVRNFL VANALYWLEE
410 420 430 440 450
FHVDGLRVDA VASMLYLDYS REPGEWLPNI YGGRENLEAV QFLQEMNATV
460 470 480 490 500
HKSHPGVMTI AEESTSWPGV TSPTWEGGLG FSMKWNMGWM NDTLEYFSHE
510 520 530 540 550
PIHRMYHHND ITFSMVYAYS EKFVLPFSHD EVVHGKGSLW TRMPGDAWNK
560 570 580 590 600
AAGLRTLYAY MYAHPGKNLL FQGQEFGQVK EWSEERSLDW GDMDGWEGEY
610 620 630 640 650
HRGIRTLVQD LNALYKDSPA LYSQDNNPAG FSWTKSDDAA NNILSFVRYG
660 670 680 690 700
ADGSKILAVF NFGGADHPSY KLGVPEGGNW KCILNTDAGI YEGEDNYLDS
710 720 730
DVMAWDTDWD GYQHSLTVHI PAMSGQLYRW EA
Length:732
Mass (Da):82,603
Last modified:July 5, 2004 - v1
Checksum:i6A734BCBFC3B0441
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248357 Genomic DNA. Translation: CAE49588.1.
RefSeqiNP_939426.1. NC_002935.2.
WP_010934779.1. NC_002935.2.

Genome annotation databases

EnsemblBacteriaiCAE49588; CAE49588; DIP1065.
GeneIDi2649325.
KEGGicdi:DIP1065.
PATRICi21483311. VBICorDip47633_1048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248357 Genomic DNA. Translation: CAE49588.1.
RefSeqiNP_939426.1. NC_002935.2.
WP_010934779.1. NC_002935.2.

3D structure databases

ProteinModelPortaliQ6NHR6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE49588; CAE49588; DIP1065.
GeneIDi2649325.
KEGGicdi:DIP1065.
PATRICi21483311. VBICorDip47633_1048.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDDADHNT.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 2 hits.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Entry informationi

Entry nameiGLGB_CORDI
AccessioniPrimary (citable) accession number: Q6NHR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: July 5, 2004
Last modified: June 24, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.