Q6NHN6 (ILVD_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	DIP1096

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 613	613	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000225384
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	197	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6NHN6 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5FA8D12F1372C3DC	FASTA	613	65,025
	10 20 30 40 50 60 MFPLRSRVTT VGRNAAGARA LWRATGTKDN EFGKPIVAIV NSYTQFVPGH VHLKNVGDIV 70 80 90 100 110 120 AEAVRAAGGV PKEFNTIAVD DGIAMGHGGM LYSLPSREII ADSVEYMVNA HTADAMVCIS 130 140 150 160 170 180 NCDKITPGML NAALRLNIPA VFVSGGPMEA GKAVVADGIA HAPTDLITAI TASAKDSVND 190 200 210 220 230 240 DQLLSIEQSA CPTCGSCSGM FTANSMNCLT EALGLSLPGN GTTLATHTAR RELFETAGKL 250 260 270 280 290 300 VVDLCQRYYG EEDESVLPRN IATKKAFINA MALDMAMGGS TNTVLHTLAA AQEGEIDFDL 310 320 330 340 350 360 SDIDALSHRI PCLSKVAPNG NYHIEDVHRA GGIPAILGEL RRRDLLEKGV HTVLYKEMDS 370 380 390 400 410 420 WLDDWDIRGG KATDKAIELF HAAPGGVRTT EPFSQSNRWD SLDTDQARGC IHDVDHAYSA 430 440 450 460 470 480 DGGLVILRGN LAPDGAVVKA AGVDESIWTF SGPARVVESQ EEAVSIILDK QVQPGDVVVI 490 500 510 520 530 540 RYEGPAGGPG MQEMLHPTSF LKGAGLGKAC ALITDGRFSG GTSGLSIGHI SPEAAHGGLI 550 560 570 580 590 600 GLVENGDTIT IDIRNRSLNL DVDDAVIEQR RAAMLEREKP WTPTNRNRPV SKALRAYASM 610 ATSADKGAVR QLD

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248357 Genomic DNA. Translation: CAE49619.1.
RefSeq	NP_939457.1. NC_002935.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2650838.
GenomeReviews	Gene locus DIP1096 in contig BX248353_GR.
KEGG	cdi:DIP1096.
NMPDR	fig|257309.1.peg.1048.
PATRIC	21483375. VBICorDip47633_1080.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	NSHNEII.
PhylomeDB	Q6NHN6.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1096-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_CORDI

Accession

Primary (citable) accession number: Q6NHN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program