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Q6NHL3 (BIOB2_CORDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase 2

EC=2.8.1.6
Gene names
Name:bioB2
Ordered Locus Names:DIP1124
OrganismCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis) [Complete proteome] [HAMAP]
Taxonomic identifier257309 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 330330Biotin synthase 2 HAMAP-Rule MF_01694
PRO_0000381326

Sites

Metal binding661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1111Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1431Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2031Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2731Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6NHL3 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 6A9E56A082D99217

FASTA33035,447
        10         20         30         40         50         60 
MTATLNTVQD LEASVLAGTA ITRDEALSLI DAPLDELSAA ADRIRAQMCG DGFDMCSIIN 

        70         80         90        100        110        120 
AKSGRCPENC TFCAQSIRYP TISVDSYPLI TADELVRQAQ ENKDKGVIRF SIVTSGRKLR 

       130        140        150        160        170        180 
RDEVRHICEG VRRIKQEVGI EVCISAGLLS AEDFQALHDA GISRVHCNLE TSRAYFPSIC 

       190        200        210        220        230        240 
ISHTFDDKIA TLQAARDEGM SLCSGGILGL GESMEDRIDM ALSARELGVN SFPVNVLVAI 

       250        260        270        280        290        300 
EGTPLAGTEQ LRPEEVQRCV AIFRFILPQA AIRLAGGREL LGDDGKACFQ SGANSAISGD 

       310        320        330 
MLTTTGTTIA SDMALVKDLG YTVTLDHSHS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248357 Genomic DNA. Translation: CAE49644.1.
RefSeqNP_939481.1. NC_002935.2.

3D structure databases

ProteinModelPortalQ6NHL3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257309.DIP1124.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE49644; CAE49644; DIP1124.
GeneID2649926.
KEGGcdi:DIP1124.
PATRIC21483433. VBICorDip47633_1106.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMATSEATYG.
OrthoDBEOG622PMP.

Enzyme and pathway databases

UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB2_CORDI
AccessionPrimary (citable) accession number: Q6NHL3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways