Q6NHE6 (BIOD_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	DIP1192

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000302501
Regions
	Nucleotide binding	12 – 17	6	ATP By similarity
	Nucleotide binding	107 – 110	4	ATP By similarity
Sites
	Metal binding	12	1	Magnesium 1 By similarity
	Metal binding	16	1	Magnesium 2 By similarity
	Metal binding	49	1	Magnesium 2 By similarity
	Metal binding	107	1	Magnesium 2 By similarity
	Binding site	41	1	Substrate By similarity
	Binding site	49	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NHE6 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2A99982A1A7843E2	FASTA	222	23,291
	10 20 30 40 50 60 MAIVVVTGTN TDVGKTIASA AVCQHYSRQG FRVVPVKPVQ TGEPKGSGDA QTIEKLTGIV 70 80 90 100 110 120 GKCFARFPEP LAPNLSAQRA GMQQLNLEEI VNKIRELDGP QTVVVVEGAG GLLVRLADSF 130 140 150 160 170 180 TIADVAAQLD APLIVVTNMA LGSLNAAELT VEAAQRRGLK VLGLIGGSMP KNPDLATSLN 190 200 210 220 VAEMEKVTGI PLWGSIAEGA GQLSKEAFCQ LVEDLHLPTM WP

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248357 Genomic DNA. Translation: CAE49719.1.
RefSeq	NP_939549.1. NC_002935.2.
3D structure databases
HSSP	HSSP built from PDB template 1DAI based on UniProtKB P13000.
ProteinModelPortal	Q6NHE6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2649747.
GenomeReviews	Gene locus DIP1192 in contig BX248353_GR.
KEGG	cdi:DIP1192.
NMPDR	fig|257309.1.peg.1140.
PATRIC	21483581. VBICorDip47633_1176.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	AEAVGWC.
PhylomeDB	Q6NHE6.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1192-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CORDI

Accession

Primary (citable) accession number: Q6NHE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program