Q6NH87 (FTHS_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	DIP1253

Organism

Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis) [Complete proteome] [HAMAP]

Taxonomic identifier

257309 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium diphtheriae

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000199342
Regions
	Nucleotide binding	62 – 69	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NH87 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 27D7F2392BABA05D	FASTA	550	58,098
	10 20 30 40 50 60 MPTDVEIAQA HTLEPITDIA NRAGVPSDAL IPYGFTKAKI DINRIASENT GKLVLVTGIS 70 80 90 100 110 120 PTPAGEGKST VLIGLSDAMR LRGHNSIVAI REPSLGPVMG IKGGAAGGGY SQIVPMEDIN 130 140 150 160 170 180 LHFTGDFHAI TAANNTLAAM IDNHIHQGNT LGIDVRRITW QRCLDVNDRC LRKVVTGLGG 190 200 210 220 230 240 KAHGVPTETG FTITAASEIM AILCLALDLT DLEARLARIV VGQTFSSEPV TVGQLNAQGA 250 260 270 280 290 300 LAALLRDAVN PNLVQTLGGT PALCHGGPFA NIAHGCNSLI ATKTALSLGD VVLTEAGFGS 310 320 330 340 350 360 DLGAEKFFDI KSRVGDLNVA ATVVVATVRS LKYNAGVPKD ELTTENLEAL ASGVVNLERH 370 380 390 400 410 420 VENIRAFGIE PIVALNKFAS DTDAEINQLK AWAETMSVQL IPVEVWAHGG QGALELADAV 430 440 450 460 470 480 AVSMQNQTSH HLYDPELGIE ASLLTIAQKI YGAADVELSK QARQDLAYLQ ENGWDRLPVC 490 500 510 520 530 540 ISKTQYSFSD DPSQLGRPEG HTLHVRNLLP RIGAGFIVAL TGDVMTMPGL PKKPAAENIG 550 VENGEIKGLF

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248357 Genomic DNA. Translation: CAE49782.1.
RefSeq	NP_939608.1. NC_002935.2.
3D structure databases
ProteinModelPortal	Q6NH87.
ModBase	Search...
Protein-protein interaction databases
STRING	257309.DIP1253.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE49782; CAE49782; DIP1253.
GeneID	2649808.
KEGG	cdi:DIP1253.
PATRIC	21483701. VBICorDip47633_1235.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
OMA	EIMAVLC.
ProtClustDB	PRK13505.
Enzyme and pathway databases
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_CORDI

Accession

Primary (citable) accession number: Q6NH87

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program