Q6NGL1 (DXR_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Short name=DXP reductoisomerase
EC=1.1.1.267

Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomerase
2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	DIP1500

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00183
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity. HAMAP MF_00183
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: EC NADPH binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_0000163640
Regions
	Nucleotide binding	8 – 37	30	NADP By similarity
Sites
	Metal binding	147	1	Divalent metal cation By similarity
	Metal binding	149	1	Divalent metal cation By similarity
	Metal binding	218	1	Divalent metal cation By similarity
	Binding site	124	1	Substrate By similarity
	Binding site	149	1	Substrate By similarity
	Binding site	173	1	Substrate By similarity
	Binding site	196	1	Substrate By similarity
	Binding site	218	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NGL1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: ADDA981B009E1406	FASTA	387	40,704
	10 20 30 40 50 60 MRKKILILGS TGSIGTQALE VIASRQDQFE VVGIAAGGRD PQLIIQQAQM LGLAADHVAV 70 80 90 100 110 120 ANEKSATQVS RALGAAVLSG RDAATDLVES VPADTVLNGL VGSMGLRATL ATIQLGEVLA 130 140 150 160 170 180 LANKESLVAG GTFVTSQAAP GQIVPVDSEH SAMAQCLRSG ASAEVSKLVL TASGGPFRGW 190 200 210 220 230 240 SREEMWDVTP EQAAAHPTWS MGQMNTLNSA TLINKGLELI EATLLFDIPA DRIEVTVHPQ 250 260 270 280 290 300 SIVHSMVTFC DGATIAQASP PSMLLSISHA LAWPHRVPEA QPALDFSQAS TWDFMPVDNE 310 320 330 340 350 360 AFPAVELARE VALKQGTYPA VYNAANEQAA AAFLRGRIKF PQIVDIVGEV VAGSSQFAGV 370 380 ASSVEEIIAH ESESRRRADA LVDKLSR

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248358 Genomic DNA. Translation: CAE50027.1.
RefSeq	NP_939846.1. NC_002935.2.
3D structure databases
ProteinModelPortal	Q6NGL1.
SMR	Q6NGL1. Positions 2-384.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2650384.
GenomeReviews	Gene locus DIP1500 in contig BX248353_GR.
KEGG	cdi:DIP1500.
NMPDR	fig|257309.1.peg.1437.
PATRIC	21484208. VBICorDip47633_1480.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG430762.
OMA	IHSMVEY.
PhylomeDB	Q6NGL1.
ProtClustDB	PRK05447.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1500-MONOMER.
Family and domain databases
HAMAP	MF_00183. DXP_reductoisom. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00099.
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_CORDI

Accession

Primary (citable) accession number: Q6NGL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program