Q6NGJ8 (RNH2_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	DIP1515

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Ribonuclease HII HAMAP MF_00052_B		PRO_0000111567
Sites
	Metal binding	40	1	Divalent metal cation By similarity
	Metal binding	41	1	Divalent metal cation By similarity
	Metal binding	134	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NGJ8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 33F29A0EFFDC6F12	FASTA	229	25,067
	10 20 30 40 50 60 MITQEHYCAL PAPKIRTLKH LRTFEVTLDK HGLGPVAGVD EAGRGSCCGP ITIAACIMPQ 70 80 90 100 110 120 RPIAQLQALT DSKKLSAAKR AELYPLIKKY ALAWSIIHIS AAEIDREGIQ HANIFGMRRA 130 140 150 160 170 180 IEKLDVAPGY VLTDAMKVPG LRCPSLPIIG GDAAVRCIAA ASVLAKHSRD LIMDDLGGKY 190 200 210 220 PEYGLEDHKG YGTKSHMDAV RHHGATPEHR YSYSNVKAAH DQWLQEKTQ

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248358 Genomic DNA. Translation: CAE50042.1.
RefSeq	NP_939861.1. NC_002935.2.
3D structure databases
ProteinModelPortal	Q6NGJ8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2650399.
GenomeReviews	Gene locus DIP1515 in contig BX248353_GR.
KEGG	cdi:DIP1515.
NMPDR	fig|257309.1.peg.1452.
PATRIC	21484240. VBICorDip47633_1496.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG584843.
OMA	RLGPTPI.
PhylomeDB	Q6NGJ8.
ProtClustDB	PRK00015.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1515-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_CORDI

Accession

Primary (citable) accession number: Q6NGJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program