Q6NGI5 (TRMD_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	DIP1530

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060363
Regions
	Region	175 – 180	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	151	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NGI5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 4B6ABDD03F1D1A09	FASTA	292	32,418
	10 20 30 40 50 60 MRLDVITIFP EYLDPLRHAL LGKAIEKDLL SVGVHDLRLW AEDAHKSVDD SPFGGGPGMV 70 80 90 100 110 120 MKPTVWGPAL DDVATMSGKA HMGAQLDSAR VHVDKPRHDE LEGIQFAGYD AAEVAEADKP 130 140 150 160 170 180 LLLVPTPAGA PFTQEDARAW SNEEHIVFAC GRYEGIDQRV IEDAKKTYRV REVSIGDYVL 190 200 210 220 230 240 IGGEVAVLVI AEAVVRLIPG VLGNTQSHQD DSFSDGLLEG PSYTKPREWR GLEVPEVLTS 250 260 270 280 290 GNHAKIERWR REQSLKRTWE VRPELLDGME LDRHDQAYVE GLRRGNTSDN LN

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248358 Genomic DNA. Translation: CAE50056.1.
RefSeq	NP_939874.1. NC_002935.2.
3D structure databases
ProteinModelPortal	Q6NGI5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2650720.
GenomeReviews	Gene locus DIP1530 in contig BX248353_GR.
KEGG	cdi:DIP1530.
NMPDR	fig|257309.1.peg.1465.
PATRIC	21484270. VBICorDip47633_1511.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285805.
OMA	VCGRFEG.
PhylomeDB	Q6NGI5.
ProtClustDB	PRK00026.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1530-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 2 hits. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_CORDI

Accession

Primary (citable) accession number: Q6NGI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program