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Q6NGD7

- SYI_CORDI

UniProt

Q6NGD7 - SYI_CORDI

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei630 – 6301ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:DIP1589
    OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
    Taxonomic identifieri257309 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000002198: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10521052Isoleucine--tRNA ligasePRO_0000098536Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi257309.DIP1589.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6NGD7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi627 – 6315"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiENRDWFE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6NGD7-1 [UniParc]FASTAAdd to Basket

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    MSDAVGGVYP RVDMSGGTNV FPDMERQVLE YWKDDETFKA SLTNREENPE     50
    YVFYDGPPFA NGLPHYGHLL TGYVKDIVPR YQTMKGKLVN RVFGWDCHGL 100
    PAELEAEKQL GIKDKGEIEA MGLESFNNYC AKSVLEYTQE WKDYVTRQAR 150
    WVDFDNGYKT MDMDFMESVM WAFKTLYDKG LIYQGFRVLP YSWAEHTPLS 200
    NQETRLDDSY KMRQDPTLTV TFPITGVKDD SAADASLVGA YALAWTTTPW 250
    TLPSNLALAV NPQVNYVEVK VGDQGAEAIR GQRVLLAEAL VGAYAKELGE 300
    DHEVLTVRPG SELVGLTYQP IFSYFADHEN AFQILAAEYV TTEDGTGIVH 350
    QAPAFGEDDM NTCKEYGIEV VIPVDMDGKF TSLVPEYQGQ LVFDANKSII 400
    ADLKAAGRVV RHQTIEHSYP HSWRSGEPLI YMALPSWFVE VTKIRDRMVE 450
    LNKEIDWMPS HIRDGQFGKW LEGARDWNIS RNRYWGSPIP VWVSDDENYP 500
    RVDVYGSLEE LERDFGVRPE SLHRPHIDEL TRPNPDDPTG KSTMRRVPEV 550
    LDCWFESGSM PFAQKHYPFE NKDWFDTHSP ADFIVEYSGQ TRGWFYTLHV 600
    LATALFDRPA FKKVVAHGIV LGDDGTKMSK SRRNYPDVNE VFNRDGSDAM 650
    RWFLMSSPIL RGGNLIVTEQ GIREGVRQAL LPMWNAYSFL QLYSSKPAQW 700
    SVDSSDVLDR YILAKLHDVV AAVGDALDNT DIARACDEVR TFCDALTNWY 750
    VRRSRDRFWA GDTEHPEAFY TLYTVLETLT RVTAPLLPMV SEVIWRGLTG 800
    ERSVHLADFP QADQFPADDD LVRAMDEVRG VCSATSSVRK AHKLRNRLPL 850
    PKVTVALPES ARLADFADII RDEVNVKEVA LTSDVDSVGR FDVVVNAKVA 900
    GPRLCKDVQR AIKAVKSGNY ERRGDTVVAD GIELVAGEFT ERLVAADPDS 950
    TTQIDGVDGL VVLDMTLTEE LEAEGWAADV IRGLQDARKA SGFEVSDRIE 1000
    VKLVVPEEKK EWALRHTDMI AGEVLATSFE VVTGEPAEHD IVAGVTATVQ 1050
    KV 1052
    Length:1,052
    Mass (Da):118,486
    Last modified:July 5, 2004 - v1
    Checksum:i0E045969A137FBCD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248358 Genomic DNA. Translation: CAE50114.1.
    RefSeqiNP_939931.1. NC_002935.2.
    WP_010935178.1. NC_002935.2.

    Genome annotation databases

    EnsemblBacteriaiCAE50114; CAE50114; DIP1589.
    GeneIDi2650778.
    KEGGicdi:DIP1589.
    PATRICi21484388. VBICorDip47633_1570.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248358 Genomic DNA. Translation: CAE50114.1 .
    RefSeqi NP_939931.1. NC_002935.2.
    WP_010935178.1. NC_002935.2.

    3D structure databases

    ProteinModelPortali Q6NGD7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257309.DIP1589.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE50114 ; CAE50114 ; DIP1589 .
    GeneIDi 2650778.
    KEGGi cdi:DIP1589.
    PATRICi 21484388. VBICorDip47633_1570.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi ENRDWFE.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

    Entry informationi

    Entry nameiSYI_CORDI
    AccessioniPrimary (citable) accession number: Q6NGD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3