Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei630 – 6301ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:DIP1589
OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Taxonomic identifieri257309 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
ProteomesiUP000002198 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10521052Isoleucine--tRNA ligasePRO_0000098536Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ6NGD7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi627 – 6315"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NGD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDAVGGVYP RVDMSGGTNV FPDMERQVLE YWKDDETFKA SLTNREENPE
60 70 80 90 100
YVFYDGPPFA NGLPHYGHLL TGYVKDIVPR YQTMKGKLVN RVFGWDCHGL
110 120 130 140 150
PAELEAEKQL GIKDKGEIEA MGLESFNNYC AKSVLEYTQE WKDYVTRQAR
160 170 180 190 200
WVDFDNGYKT MDMDFMESVM WAFKTLYDKG LIYQGFRVLP YSWAEHTPLS
210 220 230 240 250
NQETRLDDSY KMRQDPTLTV TFPITGVKDD SAADASLVGA YALAWTTTPW
260 270 280 290 300
TLPSNLALAV NPQVNYVEVK VGDQGAEAIR GQRVLLAEAL VGAYAKELGE
310 320 330 340 350
DHEVLTVRPG SELVGLTYQP IFSYFADHEN AFQILAAEYV TTEDGTGIVH
360 370 380 390 400
QAPAFGEDDM NTCKEYGIEV VIPVDMDGKF TSLVPEYQGQ LVFDANKSII
410 420 430 440 450
ADLKAAGRVV RHQTIEHSYP HSWRSGEPLI YMALPSWFVE VTKIRDRMVE
460 470 480 490 500
LNKEIDWMPS HIRDGQFGKW LEGARDWNIS RNRYWGSPIP VWVSDDENYP
510 520 530 540 550
RVDVYGSLEE LERDFGVRPE SLHRPHIDEL TRPNPDDPTG KSTMRRVPEV
560 570 580 590 600
LDCWFESGSM PFAQKHYPFE NKDWFDTHSP ADFIVEYSGQ TRGWFYTLHV
610 620 630 640 650
LATALFDRPA FKKVVAHGIV LGDDGTKMSK SRRNYPDVNE VFNRDGSDAM
660 670 680 690 700
RWFLMSSPIL RGGNLIVTEQ GIREGVRQAL LPMWNAYSFL QLYSSKPAQW
710 720 730 740 750
SVDSSDVLDR YILAKLHDVV AAVGDALDNT DIARACDEVR TFCDALTNWY
760 770 780 790 800
VRRSRDRFWA GDTEHPEAFY TLYTVLETLT RVTAPLLPMV SEVIWRGLTG
810 820 830 840 850
ERSVHLADFP QADQFPADDD LVRAMDEVRG VCSATSSVRK AHKLRNRLPL
860 870 880 890 900
PKVTVALPES ARLADFADII RDEVNVKEVA LTSDVDSVGR FDVVVNAKVA
910 920 930 940 950
GPRLCKDVQR AIKAVKSGNY ERRGDTVVAD GIELVAGEFT ERLVAADPDS
960 970 980 990 1000
TTQIDGVDGL VVLDMTLTEE LEAEGWAADV IRGLQDARKA SGFEVSDRIE
1010 1020 1030 1040 1050
VKLVVPEEKK EWALRHTDMI AGEVLATSFE VVTGEPAEHD IVAGVTATVQ

KV
Length:1,052
Mass (Da):118,486
Last modified:July 5, 2004 - v1
Checksum:i0E045969A137FBCD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248358 Genomic DNA. Translation: CAE50114.1.
RefSeqiNP_939931.1. NC_002935.2.
WP_010935178.1. NC_002935.2.

Genome annotation databases

EnsemblBacteriaiCAE50114; CAE50114; DIP1589.
GeneIDi2650778.
KEGGicdi:DIP1589.
PATRICi21484388. VBICorDip47633_1570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248358 Genomic DNA. Translation: CAE50114.1.
RefSeqiNP_939931.1. NC_002935.2.
WP_010935178.1. NC_002935.2.

3D structure databases

ProteinModelPortaliQ6NGD7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE50114; CAE50114; DIP1589.
GeneIDi2650778.
KEGGicdi:DIP1589.
PATRICi21484388. VBICorDip47633_1570.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Entry informationi

Entry nameiSYI_CORDI
AccessioniPrimary (citable) accession number: Q6NGD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: June 24, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.