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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:DIP1670
OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Taxonomic identifieri257309 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000002198 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002092411 – 1054Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST1054

Structurei

3D structure databases

ProteinModelPortaliQ6NG61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 535Adenylyl removaseUniRule annotationAdd BLAST535
Regioni541 – 1054Adenylyl transferaseUniRule annotationAdd BLAST514

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000243040.
KOiK00982.
OMAiFTVQLLQ.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

Q6NG61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKTLPSIGA LGFSNTNAEK DLCCLGWYSN DPDTVVDAPE QAAEILWHLS
60 70 80 90 100
GTADPDLALN TIIRLMEALG EQKGQLHNAL RTDPELRVKL FSLLGASTAL
110 120 130 140 150
GDHLVAHPHT WPELQRPMPT RQEMFRLMLG SVGAARASFS SESQQPQGSD
160 170 180 190 200
SDSEFSFGAD LSADDTANTD LSTPGTYRAE ITGERAEVAL KTTYRSLLLR
210 220 230 240 250
IAAADLAGTY PEDIHRTGLP EVPFVTVTKA LADLADAALT AALAVAVAHV
260 270 280 290 300
YPEGEVDTHL AVMAMGKCGA QELNYISDVD VIFVAEPVTP KAIRLAGEFI
310 320 330 340 350
RIGCACFFEV DAALRPEGKH GVLVRTLDSH VAYYKRWAET WEFQALLKHR
360 370 380 390 400
PMTGYMPLGQ AYSEKLQPMV WEASQRESFV DNVQRMRRRV LENVPAKLKN
410 420 430 440 450
LELKLGEGGL RDVEFAVQLL QLVHGRSDES LRTLSTIDAL NALIEGGYVG
460 470 480 490 500
REDGAELIRA YEFLRLLEHR LQLQKVKRTH TMPEATKTKQ LRWLARAAGF
510 520 530 540 550
KTSKLASATD EMNAMLKTSR LHISSLHRKL FYRPLLDSVV NISVGTLKLS
560 570 580 590 600
PAAAKLQLAA LGYVFPDRAM DHLHALAAGG SRKAKIQAML LPTLMEWLSE
610 620 630 640 650
TAEPDAGLLN YRKLSDAAYD RTWFLRMLRD EGVVGQRLMR ILGNSPYTAD
660 670 680 690 700
LIISAPDIVK QLGDGATRPK LLETSEDRVT KSIVAAAARH DDPDVAIAVA
710 720 730 740 750
RSLRRAELAR IASADLLQML SVQQVCRRLS YVWDAVLEAG LQAEIRASLI
760 770 780 790 800
GSTGDKNSVP PARIAVIGMG RLGGSELGYG SDADVMFVCE PTEGVSDEAA
810 820 830 840 850
VKWAIGVCDS MRSRLAKPSG DPPLDVDLGL RPEGRSGAVV RTLESYKQYY
860 870 880 890 900
ERWGEVWEIQ ALLRADFIAG DQELGARFLE MIEPLRYPEA GVSQKVIREV
910 920 930 940 950
RRMKARVDNE RLPRGADRNT HTKLGRGALT DVEWTVQLLT MMHAHEFQDL
960 970 980 990 1000
HNPSTLDSLD VIEKHAVIEP EKVEVLRQAW LTATRARNAI VLVRGKRVDQ
1010 1020 1030 1040 1050
LPQQGTQLAA VAGAAGWEPS DSQQYLDHYL KVTRRARQVV DEVFWGVDSI

EHDY
Length:1,054
Mass (Da):116,477
Last modified:July 5, 2004 - v1
Checksum:i688E75171CE61964
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248358 Genomic DNA. Translation: CAE50199.1.
RefSeqiWP_010935241.1. NC_002935.2.

Genome annotation databases

EnsemblBacteriaiCAE50199; CAE50199; DIP1670.
KEGGicdi:DIP1670.

Similar proteinsi

Entry informationi

Entry nameiGLNE_CORDI
AccessioniPrimary (citable) accession number: Q6NG61
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 5, 2004
Last modified: November 22, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families