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Protein

Glutamate 5-kinase

Gene

proB

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei23ATPUniRule annotation1
Binding sitei63SubstrateUniRule annotation1
Binding sitei150SubstrateUniRule annotation1
Binding sitei162Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi182 – 183ATPUniRule annotation2
Nucleotide bindingi222 – 228ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:DIP1777
OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Taxonomic identifieri257309 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000002198 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001096631 – 376Glutamate 5-kinaseAdd BLAST376

Structurei

3D structure databases

ProteinModelPortaliQ6NFV9.
SMRiQ6NFV9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini284 – 358PUAUniRule annotationAdd BLAST75

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246368.
KOiK00931.
OMAiTHEIRFG.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiView protein in InterPro
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiView protein in SMART
SM00359. PUA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiView protein in PROSITE
PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6NFV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAQHSSADL RDVIRNAKRI VVKIGSSSLT GENFEVSPGR IDRIVEALED
60 70 80 90 100
RMHRGSDVIV VSSGAVAAGM APLGLTSRPK DLATKQAAAS VGQVHLANAW
110 120 130 140 150
GNSFARYNRT IGQVLLTASD AGQRDRARNA QRTIDRLRLL HAVPIINEND
160 170 180 190 200
TVATSEMHFG DNDRLAAIVS HLVSADALVL LSDVDGLYDK NPADPSARFV
210 220 230 240 250
SEVRDGNDLK GVIAGDGGAV GTGGMASKVS AARLASRSGV PVLLTSADYV
260 270 280 290 300
AEALGDAEVG TVFYPKDDRL SAWKFWALYA ADTGGALRID AGAVAAVTAG
310 320 330 340 350
GNSLLAVGIT EVIGDFHAGE IVEILGPEGE IIGRGEVAYD SAVLITMLGK
360 370
QTAQLPEGLQ RPVVHADYLS DYASRA
Length:376
Mass (Da):39,361
Last modified:July 5, 2004 - v1
Checksum:iB48316665C25E7B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248359 Genomic DNA. Translation: CAE50307.1.
RefSeqiWP_003852446.1. NC_002935.2.

Genome annotation databases

EnsemblBacteriaiCAE50307; CAE50307; DIP1777.
KEGGicdi:DIP1777.

Similar proteinsi

Entry informationi

Entry nameiPROB_CORDI
AccessioniPrimary (citable) accession number: Q6NFV9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 5, 2004
Last modified: August 30, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families