Q6NFV9 (PROB_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	DIP1777

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Glutamate 5-kinase HAMAP MF_00456		PRO_0000109663
Regions
	Domain	284 – 358	75	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q6NFV9 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B48316665C25E7B0	FASTA	376	39,361
	10 20 30 40 50 60 MNAQHSSADL RDVIRNAKRI VVKIGSSSLT GENFEVSPGR IDRIVEALED RMHRGSDVIV 70 80 90 100 110 120 VSSGAVAAGM APLGLTSRPK DLATKQAAAS VGQVHLANAW GNSFARYNRT IGQVLLTASD 130 140 150 160 170 180 AGQRDRARNA QRTIDRLRLL HAVPIINEND TVATSEMHFG DNDRLAAIVS HLVSADALVL 190 200 210 220 230 240 LSDVDGLYDK NPADPSARFV SEVRDGNDLK GVIAGDGGAV GTGGMASKVS AARLASRSGV 250 260 270 280 290 300 PVLLTSADYV AEALGDAEVG TVFYPKDDRL SAWKFWALYA ADTGGALRID AGAVAAVTAG 310 320 330 340 350 360 GNSLLAVGIT EVIGDFHAGE IVEILGPEGE IIGRGEVAYD SAVLITMLGK QTAQLPEGLQ 370 RPVVHADYLS DYASRA

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248359 Genomic DNA. Translation: CAE50307.1.
RefSeq	NP_940115.1. NC_002935.2.
3D structure databases
ProteinModelPortal	Q6NFV9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2649402.
GenomeReviews	Gene locus DIP1777 in contig BX248353_GR.
KEGG	cdi:DIP1777.
NMPDR	fig|257309.1.peg.1706.
PATRIC	21484780. VBICorDip47633_1763.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG507643.
OMA	TFGDNDM.
PhylomeDB	Q6NFV9.
ProtClustDB	PRK05429.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1777-MONOMER.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00931.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. ProB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_CORDI

Accession

Primary (citable) accession number: Q6NFV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program