Q6NFN7 (NTPA_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

DIP1850

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178157
Regions
	Region	7 – 12	6	Substrate binding By similarity
	Region	72 – 73	2	Substrate binding By similarity
Sites
	Metal binding	72	1	Magnesium or manganese By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity
	Binding site	189	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NFN7 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6EA838BB675E59BB	FASTA	207	22,032
	10 20 30 40 50 60 MKVLVASNNA KKLGELRTIL ENAGLSSVEV VPLSAIDAYD EPVEDGRTFA DNALIKARAG 70 80 90 100 110 120 AHHSGLITIA DDSGFAVEEL NGMPGVLSAR WSGQHGNDAT NNELVLAQMK HVPEERRHAA 130 140 150 160 170 180 FVSVCALVTP DGDEHIVEGR WEGRMLTAPR GANGFGYDPL FVPAEEDAAG TGRTSAEMSP 190 200 AEKNAISHRG KALQQLVPII AGYSTFF

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248359 Genomic DNA. Translation: CAE50379.1.
RefSeq	NP_940187.1. NC_002935.2.
3D structure databases
ProteinModelPortal	Q6NFN7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2649225.
GenomeReviews	Gene locus DIP1850 in contig BX248353_GR.
KEGG	cdi:DIP1850.
NMPDR	fig|257309.1.peg.1778.
PATRIC	21484934. VBICorDip47633_1834.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG697237.
OMA	GVYTADW.
PhylomeDB	Q6NFN7.
ProtClustDB	PRK00120.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1850-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_CORDI

Accession

Primary (citable) accession number: Q6NFN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program