Q6NFN6 (RNPH_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	DIP1851

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Ribonuclease PH HAMAP MF_00564		PRO_0000139883

Sequences

	Sequence		Length	Mass (Da)
	Q6NFN6 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 73F96D0030D3F03E	FASTA	241	25,895
	10 20 30 40 50 60 MTDFSRADGR AVDQMRTVKI TRGFTSNPAG SVLVEFGNTR VMCTASVELG VPRFKRDSGE 70 80 90 100 110 120 GWLTAEYAML PAATAERNAR ESMRGKVKGR THEISRLIGR SLRAAVDLDE LGENTINIDC 130 140 150 160 170 180 DVLQADGGTR TASITGAYVA LADAIAVLKE QGVVPGNPLK AAVAAVSVGV IDGQVCLDLP 190 200 210 220 230 240 YEEDSRADVD MNVIMQGDRF VEIQGTGEHN TFDRDELAVI LDFAQKGCQE LFAAQKAALE Q

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248359 Genomic DNA. Translation: CAE50380.1.
RefSeq	NP_940188.1. NC_002935.2.
3D structure databases
ProteinModelPortal	Q6NFN6.
SMR	Q6NFN6. Positions 6-239.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2649226.
GenomeReviews	Gene locus DIP1851 in contig BX248353_GR.
KEGG	cdi:DIP1851.
NMPDR	fig|257309.1.peg.1779.
PATRIC	21484936. VBICorDip47633_1835.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG737187.
OMA	MLPRATG.
PhylomeDB	Q6NFN6.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1851-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_CORDI

Accession

Primary (citable) accession number: Q6NFN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program