Reviewed, UniProtKB/Swiss-Prot Q6NFM3 (COX1_CORDI)

Names and origin

Protein names

Recommended name:
    Cytochrome c oxidase subunit 1
    EC=1.9.3.1
Alternative name(s):
    Cytochrome c oxidase polypeptide I
    Cytochrome aa3 subunit 1

Gene names

Name:	ctaD
Ordered Locus Names:	DIP1864

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B By similarity.
Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Cofactor	Binds 1 copper B per subunit By similarity. Binds 2 heme groups per subunit By similarity.
Pathway	Energy metabolism; oxidative phosphorylation.
Subunit structure	Associates with subunits II, III and IV to form cytochrome c oxidase By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the heme-copper respiratory oxidase family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Copper Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from electronic annotation. Source: InterPro electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	Cytochrome c oxidase subunit 1		PRO_0000183438
Regions
	Transmembrane	43 – 63	21	Potential
	Transmembrane	83 – 103	21	Potential
	Transmembrane	122 – 142	21	Potential
	Transmembrane	171 – 191	21	Potential
	Transmembrane	214 – 234	21	Potential
	Transmembrane	259 – 279	21	Potential
	Transmembrane	292 – 312	21	Potential
	Transmembrane	316 – 336	21	Potential
	Transmembrane	360 – 380	21	Potential
	Transmembrane	399 – 419	21	Potential
	Transmembrane	434 – 454	21	Potential
	Transmembrane	477 – 497	21	Potential
Sites
	Metal binding	87	1	Iron (heme A axial ligand) Probable
	Metal binding	265	1	Copper B Probable
	Metal binding	269	1	Copper B Probable
	Metal binding	314	1	Copper B Probable
	Metal binding	315	1	Copper B Probable
	Metal binding	398	1	Iron (heme A3 axial ligand) Probable
	Metal binding	400	1	Iron (heme A axial ligand) Probable
Amino acid modifications
	Cross-link	265 ↔ 269		1'-histidyl-3'-tyrosine (His-Tyr) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NFM3-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2865A8DB72B3CA6E	FASTA	564	63,291
	10 20 30 40 50 60 MTAVAPRLEN YAEPTRPAPT GGARKGTLAW KMLTTTDHKL LGMMYIVMSF VWFFVGGLMA 70 80 90 100 110 120 LLIRAELFSP GLQFLSNEQF NQLFTLHGTI MLLAFGTPVV WGFSNYILPL QIGAPDVAFP 130 140 150 160 170 180 RLNAFGFWIT QIGVVAMLAG FLTPGGAADF GWTMYLPLAD SIHSPGVGGD FWIIGVGATG 190 200 210 220 230 240 VGTIASAVNM ITTILCMRAP GMTMFRMPIF CWNIFVASVI VLLIFPLLTA AALGVMYDRK 250 260 270 280 290 300 LGGHIYDPGN GGAILWQHLF WFFGHPEVYV LALPFFGIVS EVIPVFARKP MFGYIGLVFA 310 320 330 340 350 360 TLSIGMLSMA VWAHHMFVTG AILLPFFSFM TFLISVPTGV KFFNWLGTMW RGHISWETPM 370 380 390 400 410 420 TWTMGFLVTF LFGGLTGIML ASPPLDFHIS DTYFVVAHFH YTLFGTVVFA SYAGVYFWFP 430 440 450 460 470 480 KMTGRMLDER LGKIHFWITF VGFHGTFLVQ HWVGNEGMPR RYADYLESDG FTTLNQISTV 490 500 510 520 530 540 FSFLLGVSVI PFIWNVFKSY RYGEIVTVDD PWGYGNSLEW ATSCPPPRHN FTSLPRIRSE 550 560 RPAFELHYPH MVERMRREAH IGHH

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248359 Genomic DNA. Translation: CAE50393.1.
RefSeq	NP_940201.1.
3D structure databases
SMR	Q6NFM3. Positions 36-531.
ModBase	Search...
Genome annotation databases
GeneID	2649239.
KEGG	cdi:DIP1864.
NMPDR	fig|257309.1.peg.1792.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG603668.
OMA	NQISTVF.
PhylomeDB	Q6NFM3.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1864-MONOMER.
BRENDA	1.9.3.1. 20483.
Family and domain databases
InterPro	IPR000883. Cyt_c_oxidase_su1. IPR014241. Cyt_c_oxidase_su1_bac. [Graphical view]
Gene3D	G3DSA:1.20.210.10. COX1. 1 hit.
PANTHER	PTHR10422. COX1. 1 hit.
Pfam	PF00115. COX1. 1 hit. [Graphical view]
PRINTS	PR01165. CYCOXIDASEI.
TIGRFAMs	TIGR02891. CtaD_CoxA. 1 hit.
PROSITE	PS50855. COX1. 1 hit. PS00077. COX1_CUB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COX1_CORDI

Accession

Primary (citable) accession number: Q6NFM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)