Q6NFL4 (Q6NFL4_CORDI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193 EMBL CAE50402.1
Ordered Locus Names:	DIP1873

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 SAAS SAAS003694 RuleBase RU004252
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 NAD HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 SAAS SAAS003694 RuleBase RU003811 EMBL CAE50402.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	43 – 50	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	45	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	Q6NFL4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8FDF2061A944EF83	FASTA	272	29,928
	10 20 30 40 50 60 MRNNIIHRLG TKPTIDPATE IRNRVEFLTR YIKHSGTKGF ALGISGGQDS TLAGRLAQLA 70 80 90 100 110 120 VEQLRVAGYP AEFWAIRLPY GVQADEDDAH IALEFIQPDH SVVINIKEAT DAAARATAQA 130 140 150 160 170 180 LGLQDVGDFN KGNIKARQRM IAQYALAGEK KLLVIGTDHA AENVTGFFTK FGDGGADILP 190 200 210 220 230 240 LAGLSKRQGA ALLEHLGAPA STWEKVPTAD LEEDRPALPD EEALGVTYRE IDAYIEGNEE 250 260 270 VSPEAQQRIE RLWKIGQHKR HLPVEPDDTW WH

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248359 Genomic DNA. Translation: CAE50402.1.
RefSeq	NP_940210.1. NC_002935.2.
3D structure databases
ProteinModelPortal	Q6NFL4.
SMR	Q6NFL4. Positions 1-272.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2649248.
GenomeReviews	Gene locus DIP1873 in contig BX248353_GR.
KEGG	cdi:DIP1873.
NMPDR	fig|257309.1.peg.1801.
PATRIC	21484980. VBICorDip47633_1857.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	GLNKRRV.
PhylomeDB	Q6NFL4.
ProtClustDB	PRK00768.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q6NFL4_CORDI

Accession

Primary (citable) accession number: Q6NFL4

Entry history

Integrated into UniProtKB/TrEMBL:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)