Reviewed, UniProtKB/Swiss-Prot Q6NFC3 (SYC1_CORDI)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase 1
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase 1
      Short name=CysRS 1

Gene names

Name:	cysS1
Ordered Locus Names:	DIP1971

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Cysteinyl-tRNA synthetase 1 HAMAP MF_00041		PRO_0000159384
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	268 – 272	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	212	1	Zinc By similarity
	Metal binding	237	1	Zinc By similarity
	Metal binding	241	1	Zinc By similarity
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NFC3-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: BB58FB59BBCCEAF7	FASTA	463	51,195
	10 20 30 40 50 60 MTLRIFDTGT RSLRDFEPIR PGHASIYLCG ATPQTQPHIG HVRSGVAFDI LRRWLIAQGL 70 80 90 100 110 120 DVAFVRNVTD IDDKILTKAA ENGRPWWEWV STYEREFTWA YNQLGVLPPS VEPRATGHVT 130 140 150 160 170 180 QMVEYMQRLI DNGFAYAAEG SVYFDVAAWT AAEGSDYGSL SGNRVEEMEQ GETEITGKRG 190 200 210 220 230 240 AHDFALWKAA KPGEPSWPTP WGDGRPGWHL ECSAMATWYL GGEFDIHCGG LDLQFPHHEN 250 260 270 280 290 300 EIAQSHAAGD GFANYWMHNH WVTMSGEKMS KSLGNVLSVP NILDKVRPVE LRYYLGSAHY 310 320 330 340 350 360 RSMLEYSEGA LLEAAAGYRR IEAFLTKVAD VEAGQWTTEF EEAMNDDLAV PRALAEIHTQ 370 380 390 400 410 420 VRHGNSALAA GDVDKAHEIA ASVRAMAAVL GVDPLDEKWL DSAASDSSTN AALDVLVQAE 430 440 450 460 LQRRTEARAA KDWATADEVR DRLHAAGITV TDTPDGPTWA LNN

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248359 Genomic DNA. Translation: CAE50502.1.
RefSeq	NP_940302.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2649018.
GenomeReviews	Gene locus DIP1971 in contig BX248353_GR.
KEGG	cdi:DIP1971.
NMPDR	fig|257309.1.peg.1893.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6NFC3.
OMA	VLWKAAK.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1971-MON.
BRENDA	6.1.1.16. 20483.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC1_CORDI

Accession

Primary (citable) accession number: Q6NFC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)