Q6NFC1 (ISPD_CORDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	DIP1973

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_0000075568
Sites
	Site	17	1	Transition state stabilizer By similarity
	Site	24	1	Transition state stabilizer By similarity
	Site	159	1	Positions MEP for the nucleophilic attack By similarity
	Site	217	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NFC1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E1A44FC13B4A9AEC	FASTA	244	25,999
	10 20 30 40 50 60 MSRRVVALVA AAGKGTRLGA DLPKAYVPLR DIPLVVRSVR AMITSAVVDE VIVIISPQMQ 70 80 90 100 110 120 DYAAALLLRW GLFSADIPVR LVHGGAERAD SVWCGLQAIA DTDAVVLIHD SARALTPPGM 130 140 150 160 170 180 IARVARAVLD GNKAVIPVVP VADTIKTVHG TTVVATPPRR DLRAVQTPQG FDIAALRAAN 190 200 210 220 230 240 IAYQQQQPDF EATDDASLME WFGTPVLCVD GDPMAFKVTT PLDYALAKAV TDQAEPTIFE VPSD

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248359 Genomic DNA. Translation: CAE50504.1.
RefSeq	NP_940304.1. NC_002935.2.
3D structure databases
ProteinModelPortal	Q6NFC1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2649020.
GenomeReviews	Gene locus DIP1973 in contig BX248353_GR.
KEGG	cdi:DIP1973.
NMPDR	fig|257309.1.peg.1895.
PATRIC	21485208. VBICorDip47633_1962.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672839.
OMA	SKVIVVC.
PhylomeDB	Q6NFC1.
ProtClustDB	PRK00155.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1973-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_CORDI

Accession

Primary (citable) accession number: Q6NFC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program