Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chaperone protein ClpB

Gene

clpB

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi207 – 214ATP 1By similarity8
Nucleotide bindingi606 – 613ATP 2By similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:DIP2104
OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Taxonomic identifieri257309 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000002198 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001911141 – 849Chaperone protein ClpBAdd BLAST849

Proteomic databases

PRIDEiQ6NF05

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6NF05
SMRiQ6NF05
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 144N-terminalBy similarityAdd BLAST144
Regioni160 – 342NBD1By similarityAdd BLAST183
Regioni343 – 546LinkerBy similarityAdd BLAST204
Regioni556 – 754NBD2By similarityAdd BLAST199
Regioni755 – 849C-terminalBy similarityAdd BLAST95

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili393 – 525By similarityAdd BLAST133

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

HOGENOMiHOG000218211
KOiK03695
OMAiHHKVRIK

Family and domain databases

Gene3Di1.10.1780.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR017730 Chaperonin_ClpB
IPR019489 Clp_ATPase_C
IPR004176 Clp_N
IPR036628 Clp_N_dom_sf
IPR001270 ClpA/B
IPR018368 ClpA/B_CS1
IPR028299 ClpA/B_CS2
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF07724 AAA_2, 1 hit
PF02861 Clp_N, 2 hits
PF10431 ClpB_D2-small, 1 hit
PRINTSiPR00300 CLPPROTEASEA
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM01086 ClpB_D2-small, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
SSF81923 SSF81923, 1 hit
TIGRFAMsiTIGR03346 chaperone_ClpB, 1 hit
PROSITEiView protein in PROSITE
PS00870 CLPAB_1, 1 hit
PS00871 CLPAB_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q6NF05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGFNPTTKT QEALQEALQK ASAAGNPDIR PEHLLAAILG QEDGIAIPVL
60 70 80 90 100
RATGVDPDVV RREAEALVAK LPKAEGANLA NPNFNRDALS VLNNAQELAG
110 120 130 140 150
ELGDEYVSTE VLLAAVARGT NDAAELLTKR GATYDVIKGV FPSVRGNKKV
160 170 180 190 200
TTESPEDQFQ ALEKYSTDLT ARAREGKIDP VIGRDQEIRR VVQVLSRRTK
210 220 230 240 250
NNPVLIGEPG VGKTAIVEGL ARRIVAGDVP ESLKGKTLIS LDLGSMVAGA
260 270 280 290 300
KYRGEFEERL KAVLDEIKSA EGEIITFIDE LHTIVGAGAT GDSAMDAGNM
310 320 330 340 350
IKPLLARGEL RLVGATTLDE YRKYIEKDAA LERRFQQVFV GEPSVEDAVG
360 370 380 390 400
ILRGLKERYE VHHGVRIQDS ALVAAATLSD RYITSRFLPD KAIDLVDEAA
410 420 430 440 450
SRLRMEIDSS PQEIDELERI VRRLEIEEVA LSKETDAASK DRLIKLRQEL
460 470 480 490 500
ADEREKLGEL VARWNNEKGA INKVREAKEE LERLRSESEI AERDGDYGKV
510 520 530 540 550
AELRYGRIPE LEKQVAEAEE HTVETTMLSE EVTPDTIAEV VSAWTGIPAG
560 570 580 590 600
KMLQGETEKL LNMEAELGKR VVGQSEAVVA VSDAVRRARA GVADPNRPTG
610 620 630 640 650
SFLFLGPTGV GKTELAKALA EFMFDDDRAM VRIDMSEYGE KHAVARLVGA
660 670 680 690 700
PPGYVGYDQG GQLTEAVRRR PYTVVLFDEV EKAHPDVFDI LLQVLDEGRL
710 720 730 740 750
TDGQGRTVDF RNTVLILTSN LGAGGTKDEM MDAVKRAFKP EFVNRLDDVV
760 770 780 790 800
IFDPLSQEQL THIVEIQIAQ LAQRLAARRL TLAVSDSAKL WLAERGYEPA
810 820 830 840
YGARPLRRLI QQAIGDQLAK KLLSGEVRDG SEVHVDADLD NDGLVISAS
Length:849
Mass (Da):92,902
Last modified:July 5, 2004 - v1
Checksum:i9347D93A64A2C32A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248360 Genomic DNA Translation: CAE50634.1
RefSeqiWP_010935575.1, NC_002935.2

Genome annotation databases

EnsemblBacteriaiCAE50634; CAE50634; DIP2104
KEGGicdi:DIP2104

Similar proteinsi

Entry informationi

Entry nameiCLPB_CORDI
AccessioniPrimary (citable) accession number: Q6NF05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health