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Protein

Chaperone protein ClpB

Gene

clpB

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi207 – 214ATP 1By similarity8
Nucleotide bindingi606 – 613ATP 2By similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:DIP2104
OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Taxonomic identifieri257309 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000002198 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001911141 – 849Chaperone protein ClpBAdd BLAST849

Proteomic databases

PRIDEiQ6NF05.

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6NF05.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 144N-terminalBy similarityAdd BLAST144
Regioni160 – 342NBD1By similarityAdd BLAST183
Regioni343 – 546LinkerBy similarityAdd BLAST204
Regioni556 – 754NBD2By similarityAdd BLAST199
Regioni755 – 849C-terminalBy similarityAdd BLAST95

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili393 – 525By similarityAdd BLAST133

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

HOGENOMiHOG000218211.
KOiK03695.
OMAiWHILLAM.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR017730. Chaperonin_ClpB.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR03346. chaperone_ClpB. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NF05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGFNPTTKT QEALQEALQK ASAAGNPDIR PEHLLAAILG QEDGIAIPVL
60 70 80 90 100
RATGVDPDVV RREAEALVAK LPKAEGANLA NPNFNRDALS VLNNAQELAG
110 120 130 140 150
ELGDEYVSTE VLLAAVARGT NDAAELLTKR GATYDVIKGV FPSVRGNKKV
160 170 180 190 200
TTESPEDQFQ ALEKYSTDLT ARAREGKIDP VIGRDQEIRR VVQVLSRRTK
210 220 230 240 250
NNPVLIGEPG VGKTAIVEGL ARRIVAGDVP ESLKGKTLIS LDLGSMVAGA
260 270 280 290 300
KYRGEFEERL KAVLDEIKSA EGEIITFIDE LHTIVGAGAT GDSAMDAGNM
310 320 330 340 350
IKPLLARGEL RLVGATTLDE YRKYIEKDAA LERRFQQVFV GEPSVEDAVG
360 370 380 390 400
ILRGLKERYE VHHGVRIQDS ALVAAATLSD RYITSRFLPD KAIDLVDEAA
410 420 430 440 450
SRLRMEIDSS PQEIDELERI VRRLEIEEVA LSKETDAASK DRLIKLRQEL
460 470 480 490 500
ADEREKLGEL VARWNNEKGA INKVREAKEE LERLRSESEI AERDGDYGKV
510 520 530 540 550
AELRYGRIPE LEKQVAEAEE HTVETTMLSE EVTPDTIAEV VSAWTGIPAG
560 570 580 590 600
KMLQGETEKL LNMEAELGKR VVGQSEAVVA VSDAVRRARA GVADPNRPTG
610 620 630 640 650
SFLFLGPTGV GKTELAKALA EFMFDDDRAM VRIDMSEYGE KHAVARLVGA
660 670 680 690 700
PPGYVGYDQG GQLTEAVRRR PYTVVLFDEV EKAHPDVFDI LLQVLDEGRL
710 720 730 740 750
TDGQGRTVDF RNTVLILTSN LGAGGTKDEM MDAVKRAFKP EFVNRLDDVV
760 770 780 790 800
IFDPLSQEQL THIVEIQIAQ LAQRLAARRL TLAVSDSAKL WLAERGYEPA
810 820 830 840
YGARPLRRLI QQAIGDQLAK KLLSGEVRDG SEVHVDADLD NDGLVISAS
Length:849
Mass (Da):92,902
Last modified:July 5, 2004 - v1
Checksum:i9347D93A64A2C32A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248360 Genomic DNA. Translation: CAE50634.1.
RefSeqiWP_010935575.1. NC_002935.2.

Genome annotation databases

EnsemblBacteriaiCAE50634; CAE50634; DIP2104.
GeneIDi2649571.
KEGGicdi:DIP2104.
PATRICi21485489. VBICorDip47633_2102.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248360 Genomic DNA. Translation: CAE50634.1.
RefSeqiWP_010935575.1. NC_002935.2.

3D structure databases

ProteinModelPortaliQ6NF05.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ6NF05.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE50634; CAE50634; DIP2104.
GeneIDi2649571.
KEGGicdi:DIP2104.
PATRICi21485489. VBICorDip47633_2102.

Phylogenomic databases

HOGENOMiHOG000218211.
KOiK03695.
OMAiWHILLAM.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR017730. Chaperonin_ClpB.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR03346. chaperone_ClpB. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLPB_CORDI
AccessioniPrimary (citable) accession number: Q6NF05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: October 5, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.