Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q6NET5

- PCKG_CORDI

UniProt

Q6NET5 - PCKG_CORDI

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei82 – 821SubstrateUniRule annotation
    Binding sitei223 – 2231Substrate; via amide nitrogenUniRule annotation
    Metal bindingi230 – 2301ManganeseUniRule annotation
    Binding sitei230 – 2301SubstrateUniRule annotation
    Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
    Binding sitei272 – 2721SubstrateUniRule annotation
    Active sitei274 – 2741UniRule annotation
    Metal bindingi297 – 2971ManganeseUniRule annotation
    Binding sitei389 – 3891GTPUniRule annotation
    Binding sitei420 – 4201GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi273 – 2786GTPUniRule annotation
    Nucleotide bindingi514 – 5174GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Synonyms:pck
    Ordered Locus Names:DIP2180
    OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
    Taxonomic identifieri257309 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000002198: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 612612Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103601Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi257309.DIP2180.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6NET5.
    SMRiQ6NET5. Positions 13-608.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni387 – 3893Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiIDWHGND.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6NET5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTVSIKGLE GEAPTKNEVL LNWIAENVEL FQPDRVVFAD GSQEEWDRLT    50
    TQLVESGTLI RLNEEKRPNS FLARSNPADV ARVESRTFIC TPTPEGAGPT 100
    NHWADPAEMK AELTERFRGS MKGRTMYVVP FCMGPINDPA PKLGVQLTDS 150
    EYVVLSMRIM TRMGAEALAK IGDTGSFVPC LHSVGAPLEP GQEDVAWPCN 200
    EEKYITHFPE TKEIWSYGSG YGGNAILAKK CYALRIASVM AKEEGWMAEH 250
    MLILKLTSPE KKVYYIAAAF PSACGKTNLA MLQPTIDGWS AEVVGDDIAW 300
    LRFGEDGRLY AINPENGFFG VAPGTNYSSN PNAMRTMEPG NTLFTNVALT 350
    DDGDIWWEDL ENMPEHLTDW LGNDWTPESS QPSSHPNSRY CVPLAQCPVA 400
    AEEYNDPQGV PISAILFGGR RPDTVPLVSQ AHNWDHATMI GALLSSGQTA 450
    AAEGTVGALR HDPMAMLPFI GYNAADYLQH WIDMGKKGGD KMPEVFLVNW 500
    FRRGDDGRFL WPGFGDNSRV LKWIVDRIEG RVEADETIAG YTARVEDLDL 550
    TGLDTPIEDI REALSAPAAQ WKSDLADNEE YLTFLGSKGS AVPAEVLEQF 600
    EALKARVAAA ES 612
    Length:612
    Mass (Da):67,353
    Last modified:July 5, 2004 - v1
    Checksum:iE3E5D0543E74F60E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248360 Genomic DNA. Translation: CAE50707.1.
    RefSeqiNP_940490.1. NC_002935.2.
    WP_010935642.1. NC_002935.2.

    Genome annotation databases

    EnsemblBacteriaiCAE50707; CAE50707; DIP2180.
    GeneIDi2648926.
    KEGGicdi:DIP2180.
    PATRICi21485661. VBICorDip47633_2184.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248360 Genomic DNA. Translation: CAE50707.1 .
    RefSeqi NP_940490.1. NC_002935.2.
    WP_010935642.1. NC_002935.2.

    3D structure databases

    ProteinModelPortali Q6NET5.
    SMRi Q6NET5. Positions 13-608.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 257309.DIP2180.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE50707 ; CAE50707 ; DIP2180 .
    GeneIDi 2648926.
    KEGGi cdi:DIP2180.
    PATRICi 21485661. VBICorDip47633_2184.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi IDWHGND.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

    Entry informationi

    Entry nameiPCKG_CORDI
    AccessioniPrimary (citable) accession number: Q6NET5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3