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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathway: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821SubstrateUniRule annotation
Binding sitei223 – 2231Substrate; via amide nitrogenUniRule annotation
Metal bindingi230 – 2301ManganeseUniRule annotation
Binding sitei230 – 2301SubstrateUniRule annotation
Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
Binding sitei272 – 2721SubstrateUniRule annotation
Active sitei274 – 2741UniRule annotation
Metal bindingi297 – 2971ManganeseUniRule annotation
Binding sitei389 – 3891GTPUniRule annotation
Binding sitei420 – 4201GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi273 – 2786GTPUniRule annotation
Nucleotide bindingi514 – 5174GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Synonyms:pck
Ordered Locus Names:DIP2180
OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Taxonomic identifieri257309 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
ProteomesiUP000002198 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 612612Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103601Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ6NET5.
SMRiQ6NET5. Positions 13-608.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni387 – 3893Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NET5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTVSIKGLE GEAPTKNEVL LNWIAENVEL FQPDRVVFAD GSQEEWDRLT
60 70 80 90 100
TQLVESGTLI RLNEEKRPNS FLARSNPADV ARVESRTFIC TPTPEGAGPT
110 120 130 140 150
NHWADPAEMK AELTERFRGS MKGRTMYVVP FCMGPINDPA PKLGVQLTDS
160 170 180 190 200
EYVVLSMRIM TRMGAEALAK IGDTGSFVPC LHSVGAPLEP GQEDVAWPCN
210 220 230 240 250
EEKYITHFPE TKEIWSYGSG YGGNAILAKK CYALRIASVM AKEEGWMAEH
260 270 280 290 300
MLILKLTSPE KKVYYIAAAF PSACGKTNLA MLQPTIDGWS AEVVGDDIAW
310 320 330 340 350
LRFGEDGRLY AINPENGFFG VAPGTNYSSN PNAMRTMEPG NTLFTNVALT
360 370 380 390 400
DDGDIWWEDL ENMPEHLTDW LGNDWTPESS QPSSHPNSRY CVPLAQCPVA
410 420 430 440 450
AEEYNDPQGV PISAILFGGR RPDTVPLVSQ AHNWDHATMI GALLSSGQTA
460 470 480 490 500
AAEGTVGALR HDPMAMLPFI GYNAADYLQH WIDMGKKGGD KMPEVFLVNW
510 520 530 540 550
FRRGDDGRFL WPGFGDNSRV LKWIVDRIEG RVEADETIAG YTARVEDLDL
560 570 580 590 600
TGLDTPIEDI REALSAPAAQ WKSDLADNEE YLTFLGSKGS AVPAEVLEQF
610
EALKARVAAA ES
Length:612
Mass (Da):67,353
Last modified:July 5, 2004 - v1
Checksum:iE3E5D0543E74F60E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248360 Genomic DNA. Translation: CAE50707.1.
RefSeqiNP_940490.1. NC_002935.2.
WP_010935642.1. NC_002935.2.

Genome annotation databases

EnsemblBacteriaiCAE50707; CAE50707; DIP2180.
GeneIDi2648926.
KEGGicdi:DIP2180.
PATRICi21485661. VBICorDip47633_2184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248360 Genomic DNA. Translation: CAE50707.1.
RefSeqiNP_940490.1. NC_002935.2.
WP_010935642.1. NC_002935.2.

3D structure databases

ProteinModelPortaliQ6NET5.
SMRiQ6NET5. Positions 13-608.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE50707; CAE50707; DIP2180.
GeneIDi2648926.
KEGGicdi:DIP2180.
PATRICi21485661. VBICorDip47633_2184.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Entry informationi

Entry nameiPCKG_CORDI
AccessioniPrimary (citable) accession number: Q6NET5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: July 5, 2004
Last modified: June 24, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.