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Q6NET5

- PCKG_CORDI

UniProt

Q6NET5 - PCKG_CORDI

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn(2+) ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821SubstrateUniRule annotation
Binding sitei223 – 2231Substrate; via amide nitrogenUniRule annotation
Metal bindingi230 – 2301ManganeseUniRule annotation
Binding sitei230 – 2301SubstrateUniRule annotation
Metal bindingi250 – 2501Manganese; via tele nitrogenUniRule annotation
Binding sitei272 – 2721SubstrateUniRule annotation
Active sitei274 – 2741UniRule annotation
Metal bindingi297 – 2971ManganeseUniRule annotation
Binding sitei389 – 3891GTPUniRule annotation
Binding sitei420 – 4201GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi273 – 2786GTPUniRule annotation
Nucleotide bindingi514 – 5174GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Synonyms:pck
Ordered Locus Names:DIP2180
OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Taxonomic identifieri257309 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000002198: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 612612Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103601Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi257309.DIP2180.

Structurei

3D structure databases

ProteinModelPortaliQ6NET5.
SMRiQ6NET5. Positions 13-608.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni387 – 3893Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiIDWHGND.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NET5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTVSIKGLE GEAPTKNEVL LNWIAENVEL FQPDRVVFAD GSQEEWDRLT
60 70 80 90 100
TQLVESGTLI RLNEEKRPNS FLARSNPADV ARVESRTFIC TPTPEGAGPT
110 120 130 140 150
NHWADPAEMK AELTERFRGS MKGRTMYVVP FCMGPINDPA PKLGVQLTDS
160 170 180 190 200
EYVVLSMRIM TRMGAEALAK IGDTGSFVPC LHSVGAPLEP GQEDVAWPCN
210 220 230 240 250
EEKYITHFPE TKEIWSYGSG YGGNAILAKK CYALRIASVM AKEEGWMAEH
260 270 280 290 300
MLILKLTSPE KKVYYIAAAF PSACGKTNLA MLQPTIDGWS AEVVGDDIAW
310 320 330 340 350
LRFGEDGRLY AINPENGFFG VAPGTNYSSN PNAMRTMEPG NTLFTNVALT
360 370 380 390 400
DDGDIWWEDL ENMPEHLTDW LGNDWTPESS QPSSHPNSRY CVPLAQCPVA
410 420 430 440 450
AEEYNDPQGV PISAILFGGR RPDTVPLVSQ AHNWDHATMI GALLSSGQTA
460 470 480 490 500
AAEGTVGALR HDPMAMLPFI GYNAADYLQH WIDMGKKGGD KMPEVFLVNW
510 520 530 540 550
FRRGDDGRFL WPGFGDNSRV LKWIVDRIEG RVEADETIAG YTARVEDLDL
560 570 580 590 600
TGLDTPIEDI REALSAPAAQ WKSDLADNEE YLTFLGSKGS AVPAEVLEQF
610
EALKARVAAA ES
Length:612
Mass (Da):67,353
Last modified:July 5, 2004 - v1
Checksum:iE3E5D0543E74F60E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248360 Genomic DNA. Translation: CAE50707.1.
RefSeqiNP_940490.1. NC_002935.2.
WP_010935642.1. NC_002935.2.

Genome annotation databases

EnsemblBacteriaiCAE50707; CAE50707; DIP2180.
GeneIDi2648926.
KEGGicdi:DIP2180.
PATRICi21485661. VBICorDip47633_2184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248360 Genomic DNA. Translation: CAE50707.1 .
RefSeqi NP_940490.1. NC_002935.2.
WP_010935642.1. NC_002935.2.

3D structure databases

ProteinModelPortali Q6NET5.
SMRi Q6NET5. Positions 13-608.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 257309.DIP2180.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE50707 ; CAE50707 ; DIP2180 .
GeneIDi 2648926.
KEGGi cdi:DIP2180.
PATRICi 21485661. VBICorDip47633_2184.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi IDWHGND.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Entry informationi

Entry nameiPCKG_CORDI
AccessioniPrimary (citable) accession number: Q6NET5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3