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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway:iN(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891S-adenosyl-L-methionineUniRule annotation
Binding sitei114 – 1141S-adenosyl-L-methionineUniRule annotation
Binding sitei141 – 1411S-adenosyl-L-methionineUniRule annotation
Active sitei164 – 1641By similarity
Binding sitei164 – 1641S-adenosyl-L-methionineUniRule annotation
Binding sitei168 – 1681SubstrateUniRule annotation
Binding sitei200 – 2001SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:DIP2181
OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Taxonomic identifieri257309 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
ProteomesiUP000002198 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259tRNA (guanine-N(7)-)-methyltransferasePRO_0000171322Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ6NET4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni238 – 2414Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiKFEDKAH.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NET4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTDNSDKN TKPTGYRPPQ TDFNTEFGNN LDYPRLGSVS FRRGTLTDNQ
60 70 80 90 100
ESLWDANWPI LGKDLTDAED QRIDVAEWFG RCGHKTILEI GSGTGTSTAA
110 120 130 140 150
MAPLEADTNI IAVELYKPGL AKLLGAVVRG DISNVRMIRG DGVEVLTRML
160 170 180 190 200
PEESLDGVRI YFPDPWPKAR HHKRRIIQSG VLNLIASRLK PGGVLHVATD
210 220 230 240 250
HADYAEWITE LVNVEPQLEF MGWPWDECPQ LTDRQVITKF EGKGLDKDHT

ITEFLWRRK
Length:259
Mass (Da):29,200
Last modified:July 5, 2004 - v1
Checksum:iD4D85C6B0C780F2B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248360 Genomic DNA. Translation: CAE50708.1.
RefSeqiWP_010935643.1. NC_002935.2.

Genome annotation databases

EnsemblBacteriaiCAE50708; CAE50708; DIP2181.
GeneIDi2648927.
KEGGicdi:DIP2181.
PATRICi21485663. VBICorDip47633_2185.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248360 Genomic DNA. Translation: CAE50708.1.
RefSeqiWP_010935643.1. NC_002935.2.

3D structure databases

ProteinModelPortaliQ6NET4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE50708; CAE50708; DIP2181.
GeneIDi2648927.
KEGGicdi:DIP2181.
PATRICi21485663. VBICorDip47633_2185.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiKFEDKAH.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Entry informationi

Entry nameiTRMB_CORDI
AccessioniPrimary (citable) accession number: Q6NET4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: July 22, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.