Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q6NET4 (TRMB_CORDI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:DIP2181
OrganismCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis) [Complete proteome] [HAMAP]
Taxonomic identifier257309 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 259259tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171322

Regions

Region238 – 2414Substrate binding By similarity

Sites

Active site1641 By similarity
Binding site891S-adenosyl-L-methionine By similarity
Binding site1141S-adenosyl-L-methionine By similarity
Binding site1411S-adenosyl-L-methionine By similarity
Binding site1641S-adenosyl-L-methionine By similarity
Binding site1681Substrate By similarity
Binding site2001Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6NET4 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: D4D85C6B0C780F2B

FASTA25929,200
        10         20         30         40         50         60 
MSNTDNSDKN TKPTGYRPPQ TDFNTEFGNN LDYPRLGSVS FRRGTLTDNQ ESLWDANWPI 

        70         80         90        100        110        120 
LGKDLTDAED QRIDVAEWFG RCGHKTILEI GSGTGTSTAA MAPLEADTNI IAVELYKPGL 

       130        140        150        160        170        180 
AKLLGAVVRG DISNVRMIRG DGVEVLTRML PEESLDGVRI YFPDPWPKAR HHKRRIIQSG 

       190        200        210        220        230        240 
VLNLIASRLK PGGVLHVATD HADYAEWITE LVNVEPQLEF MGWPWDECPQ LTDRQVITKF 

       250 
EGKGLDKDHT ITEFLWRRK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248360 Genomic DNA. Translation: CAE50708.1.
RefSeqNP_940491.1. NC_002935.2.

3D structure databases

ProteinModelPortalQ6NET4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257309.DIP2181.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE50708; CAE50708; DIP2181.
GeneID2648927.
KEGGcdi:DIP2181.
PATRIC21485663. VBICorDip47633_2185.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMALHIATDH.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CORDI
AccessionPrimary (citable) accession number: Q6NET4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways