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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei732 – 7321ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:DIP2320
OrganismiCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)
Taxonomic identifieri257309 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
ProteomesiUP000002198 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 960960Leucine--tRNA ligasePRO_0000152005Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ6NEF5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi71 – 8212"HIGH" regionAdd
BLAST
Motifi729 – 7335"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NEF5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMTNPSGNT PDTSAGPAFR YTAELAGEIE RSWQQYWIDN GTFNAPNPVG
60 70 80 90 100
DLAVSGDKLP EDKLFVQDMF PYPSGAGLHV GHPLGYIATD VFARFNRMLG
110 120 130 140 150
KNVLHTLGYD AFGLPAEQYA IQTGTHPRTT TMANIANMQR QLGALGLGHD
160 170 180 190 200
PRRSVATTDP EFYKWTQWIF LQIFNAWFDT KQQKARPISE LIPLLESGEV
210 220 230 240 250
ALPEGFEGSA DSYSELDAVE KAKVVDEFRL VYRSHSMVNW CPGLGTVLAN
260 270 280 290 300
EEVTADGRSE RGNFPVFRKK LSQWMMRITA YSDRLIDDLD LLDWPDKVKS
310 320 330 340 350
MQRNWIGRSR GAEVDFDALG HTITVFTTRP DTLFGASYMV LAPEHELVDA
360 370 380 390 400
LVAAGTNSYE GIDPRWTFGQ ATPAEAVKAY RASIAAKSDL ERQENKEKTG
410 420 430 440 450
VYLGVNAVNP VNGESIPVFI GDYVLTGYGT GAIMAVPAHD TRDYEFATEF
460 470 480 490 500
GLPIREVVAG GNIAEAAYTE SGAAVNSAND QGLDINGLDK QEAIAQVIEW
510 520 530 540 550
LVDKQKGSEK IQYKLRDWLF ARQRYWGEPF PVVYDENGLA HALPESMLPV
560 570 580 590 600
ELPEVEDYKP VSFDPDDADS EPHPPLAKAT EWTQVELDLG DGLKKYTRDT
610 620 630 640 650
NVMPQWAGSS WYQLRYIDPT NSEAFCDIEN ERYWTGPRSA EDSGGVDLYV
660 670 680 690 700
GGVEHAVLHL LYSRFWHKVL FDLGFVTSRE PYRRLFNQGY IQAFAYTDSR
710 720 730 740 750
GVYVPAEEVE EKDGKFYYQG EEVNQEYGKM GKSLKNAVAP DDICRDFGAD
760 770 780 790 800
TLRVYEMAMG PLDTSRPWST KDVIGAHRFL QRLWRLVVSE DDGSIVVTDA
810 820 830 840 850
ALTDDDLKQL HRTIAGMRDD YEGLRINTVV AKAIEYVNYL TKAYGSTGAP
860 870 880 890 900
RAAVEPLVIM VAAVAPHIAE ELWKRLGHND TITFVPFPEY EDKWLVDDEV
910 920 930 940 950
EMPVQINGKV RARIMVPADA SQDQISEIAL ASEAVATHIE GKNVIKKIVV
960
SGRMVNLVVK
Length:960
Mass (Da):107,162
Last modified:July 5, 2004 - v1
Checksum:i51819FA7BEB071F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248360 Genomic DNA. Translation: CAE50842.1.

Genome annotation databases

EnsemblBacteriaiCAE50842; CAE50842; DIP2320.
KEGGicdi:DIP2320.
PATRICi21485957. VBICorDip47633_2329.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248360 Genomic DNA. Translation: CAE50842.1.

3D structure databases

ProteinModelPortaliQ6NEF5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE50842; CAE50842; DIP2320.
KEGGicdi:DIP2320.
PATRICi21485957. VBICorDip47633_2329.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Entry informationi

Entry nameiSYL_CORDI
AccessioniPrimary (citable) accession number: Q6NEF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: July 22, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.