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Q6NEF5 (SYL_CORDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:DIP2320
OrganismCorynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis) [Complete proteome] [HAMAP]
Taxonomic identifier257309 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length960 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 960960Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152005

Regions

Motif71 – 8212"HIGH" region HAMAP-Rule MF_00049
Motif729 – 7335"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7321ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6NEF5 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 51819FA7BEB071F8

FASTA960107,162
        10         20         30         40         50         60 
MTMTNPSGNT PDTSAGPAFR YTAELAGEIE RSWQQYWIDN GTFNAPNPVG DLAVSGDKLP 

        70         80         90        100        110        120 
EDKLFVQDMF PYPSGAGLHV GHPLGYIATD VFARFNRMLG KNVLHTLGYD AFGLPAEQYA 

       130        140        150        160        170        180 
IQTGTHPRTT TMANIANMQR QLGALGLGHD PRRSVATTDP EFYKWTQWIF LQIFNAWFDT 

       190        200        210        220        230        240 
KQQKARPISE LIPLLESGEV ALPEGFEGSA DSYSELDAVE KAKVVDEFRL VYRSHSMVNW 

       250        260        270        280        290        300 
CPGLGTVLAN EEVTADGRSE RGNFPVFRKK LSQWMMRITA YSDRLIDDLD LLDWPDKVKS 

       310        320        330        340        350        360 
MQRNWIGRSR GAEVDFDALG HTITVFTTRP DTLFGASYMV LAPEHELVDA LVAAGTNSYE 

       370        380        390        400        410        420 
GIDPRWTFGQ ATPAEAVKAY RASIAAKSDL ERQENKEKTG VYLGVNAVNP VNGESIPVFI 

       430        440        450        460        470        480 
GDYVLTGYGT GAIMAVPAHD TRDYEFATEF GLPIREVVAG GNIAEAAYTE SGAAVNSAND 

       490        500        510        520        530        540 
QGLDINGLDK QEAIAQVIEW LVDKQKGSEK IQYKLRDWLF ARQRYWGEPF PVVYDENGLA 

       550        560        570        580        590        600 
HALPESMLPV ELPEVEDYKP VSFDPDDADS EPHPPLAKAT EWTQVELDLG DGLKKYTRDT 

       610        620        630        640        650        660 
NVMPQWAGSS WYQLRYIDPT NSEAFCDIEN ERYWTGPRSA EDSGGVDLYV GGVEHAVLHL 

       670        680        690        700        710        720 
LYSRFWHKVL FDLGFVTSRE PYRRLFNQGY IQAFAYTDSR GVYVPAEEVE EKDGKFYYQG 

       730        740        750        760        770        780 
EEVNQEYGKM GKSLKNAVAP DDICRDFGAD TLRVYEMAMG PLDTSRPWST KDVIGAHRFL 

       790        800        810        820        830        840 
QRLWRLVVSE DDGSIVVTDA ALTDDDLKQL HRTIAGMRDD YEGLRINTVV AKAIEYVNYL 

       850        860        870        880        890        900 
TKAYGSTGAP RAAVEPLVIM VAAVAPHIAE ELWKRLGHND TITFVPFPEY EDKWLVDDEV 

       910        920        930        940        950        960 
EMPVQINGKV RARIMVPADA SQDQISEIAL ASEAVATHIE GKNVIKKIVV SGRMVNLVVK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248360 Genomic DNA. Translation: CAE50842.1.
RefSeqNP_940621.1. NC_002935.2.

3D structure databases

ProteinModelPortalQ6NEF5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257309.DIP2320.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE50842; CAE50842; DIP2320.
GeneID2649143.
KEGGcdi:DIP2320.
PATRIC21485957. VBICorDip47633_2329.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAYARYHRM.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CORDI
AccessionPrimary (citable) accession number: Q6NEF5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries