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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei635 – 6351ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:RPA0287
OrganismiRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Taxonomic identifieri258594 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
ProteomesiUP000001426: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 876876Leucine--tRNA ligasePRO_0000152073Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi258594.RPA0287.

Structurei

3D structure databases

ProteinModelPortaliQ6ND22.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi632 – 6365"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.
PhylomeDBiQ6ND22.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6ND22-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNERYNARE SEPKWQAKWD EAKIFATRND DLRKKYYVLE MFPYPSGRIH
60 70 80 90 100
MGHVRNYTMG DVVARTMRAR GYNVLHPMGW DAFGLPAENA AIERKVAPKA
110 120 130 140 150
WTYDNIAAMK KQLQTMGLSL DWAREFATCD PSYYKHQQKM FLDFLKVGLV
160 170 180 190 200
EREKRKLNWD PVDMTVLANE QVIDGRGWRS GAVVELREMN QWVFKITKYA
210 220 230 240 250
QELLDALDTL DRWPDKVRLM QRNWIGRSEG LMVRFALDSA TTPAGETELK
260 270 280 290 300
IFTTRPDTLF GAKFMAIAAD HPLAQAAAAK APKVAAFIDD CKKRGTAQAE
310 320 330 340 350
IDTAEKQGID TGIRAVHPFD PSWKLPVYVA NFVLMEYGTG AIFGCPAHDQ
360 370 380 390 400
RDLDFVNKYQ LGNTPVVCPE GQDPASFVIT DTAYDGEGRM INSRFLDGKT
410 420 430 440 450
IAEAKEEVAK RLETEQLAGA PVGARKVNFR LRDWGISRQR YWGCPIPIIH
460 470 480 490 500
CPTCDVVPVP DADLPVVLPE DVSFDKPGNA LDHHPTWKHV TCPKCGGKAV
510 520 530 540 550
RETDTMDTFV DSSWYFARFT DPWNTEAPTT PDVVNRMMPV DQYIGGVEHA
560 570 580 590 600
ILHLLYSRFF TRAMKAAGHI DIQHDEPFAG LFTQGMVVHE TYRKADGHFA
610 620 630 640 650
SPAEISITVE GDTRRATLLD GGSPVEIGPI EKMSKSKRNT VDPDDIIGTY
660 670 680 690 700
GADTARWFML SDSPPDRDVI WSEEGVKGAS RFVQRLWRMV NDAAPIAASA
710 720 730 740 750
PAERPASFGA DALTLRKAAH GALDKVLSGI ERLAFNVSLA HIREFSNTLG
760 770 780 790 800
DALARSQTPS PDLAWAIRES TVILVQLFHP MMPHLAEECW TVLGQTGLVS
810 820 830 840 850
EALWPPIEQD LLVEDSITLP VQVNGKKRGD VTVPRDAPTS EIEAAVLALD
860 870
TVKQALGGKP VRKVIVVPQR IVNVVG
Length:876
Mass (Da):97,725
Last modified:July 5, 2004 - v1
Checksum:i8FDD1478FE408442
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572593 Genomic DNA. Translation: CAE25731.1.
RefSeqiNP_945640.1. NC_005296.1.

Genome annotation databases

EnsemblBacteriaiCAE25731; CAE25731; RPA0287.
GeneIDi2689342.
KEGGirpa:RPA0287.
PATRICi23284707. VBIRhoPal84835_0303.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572593 Genomic DNA. Translation: CAE25731.1.
RefSeqiNP_945640.1. NC_005296.1.

3D structure databases

ProteinModelPortaliQ6ND22.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi258594.RPA0287.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE25731; CAE25731; RPA0287.
GeneIDi2689342.
KEGGirpa:RPA0287.
PATRICi23284707. VBIRhoPal84835_0303.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.
PhylomeDBiQ6ND22.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-98 / CGA009.

Entry informationi

Entry nameiSYL_RHOPA
AccessioniPrimary (citable) accession number: Q6ND22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.