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Protein

dITP/XTP pyrophosphatase

Gene

RPA0328

Organism
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

XTP + H2O = XMP + diphosphate.UniRule annotation
dITP + H2O = dIMP + diphosphate.UniRule annotation
ITP + H2O = IMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45MagnesiumUniRule annotation1
Active sitei74Proton acceptorUniRule annotation1
Metal bindingi74MagnesiumUniRule annotation1
Binding sitei75Substrate; via amide nitrogenUniRule annotation1
Binding sitei183SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRPAL258594:G1GT2-340-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:RPA0328
OrganismiRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Taxonomic identifieri258594 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
Proteomesi
  • UP000001426 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001782201 – 210dITP/XTP pyrophosphataseAdd BLAST210

Proteomic databases

PRIDEiQ6NCY9

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi258594.RPA0328

Structurei

3D structure databases

ProteinModelPortaliQ6NCY9
SMRiQ6NCY9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 18Substrate bindingUniRule annotation6
Regioni160 – 163Substrate bindingUniRule annotation4
Regioni195 – 196Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108V82 Bacteria
COG0127 LUCA
HOGENOMiHOG000293319
KOiK02428
OMAiYDPIFQP
OrthoDBiPOG091H02BP
PhylomeDBiQ6NCY9

Family and domain databases

CDDicd00515 HAM1, 1 hit
Gene3Di3.90.950.10, 1 hit
HAMAPiMF_01405 Non_canon_purine_NTPase, 1 hit
InterProiView protein in InterPro
IPR020922 dITP/XTP_pyrophosphatase
IPR002637 Ham1p-like
IPR029001 ITPase-like_fam
PANTHERiPTHR11067 PTHR11067, 1 hit
PfamiView protein in Pfam
PF01725 Ham1p_like, 1 hit
SUPFAMiSSF52972 SSF52972, 1 hit
TIGRFAMsiTIGR00042 TIGR00042, 1 hit

Sequencei

Sequence statusi: Complete.

Q6NCY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRRITGKLV IATHNPGKLA EMRELLAPYG IEAISAGELG LAEPDETGDS
60 70 80 90 100
FQANARIKAE AAAKAAQLPA FADDSGLAVD ALDGAPGIYS ARWAGDAKDF
110 120 130 140 150
AGAMARIERL LQERGATAPE RRTAHFVSAL CVAWPDGHIE EVEARADGTL
160 170 180 190 200
VWPPRGTAGF GYDPVFLPEG HSRTFGEMTS IEKHGLPPLG LGLSHRAKAF
210
VKLAEICLAG
Length:210
Mass (Da):22,219
Last modified:July 5, 2004 - v1
Checksum:i41BEC9DC18A2567E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572594 Genomic DNA Translation: CAE25772.1
RefSeqiWP_011155896.1, NC_005296.1

Genome annotation databases

EnsemblBacteriaiCAE25772; CAE25772; RPA0328
KEGGirpa:RPA0328

Similar proteinsi

Entry informationi

Entry nameiIXTPA_RHOPA
AccessioniPrimary (citable) accession number: Q6NCY9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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