Reviewed, UniProtKB/Swiss-Prot Q6NCX0 (ACEK_RHOPA)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	RPA0347

Organism

Rhodopseudomonas palustris [Complete proteome] [HAMAP]

Taxonomic identifier

1076 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	610 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 610	610	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288298
Regions
	Nucleotide binding	359 – 365	7	ATP By similarity
Sites
	Active site	419	1	By similarity
	Binding site	380	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NCX0-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2EC20E5CC0434057	FASTA	610	67,975
	10 20 30 40 50 60 MTATASHTRA RPLGASEIEH ATRIAEPDFD LLDALYRTDD PDDQARLLAR VVLSAFDNYY 70 80 90 100 110 120 AVSRRIPALA QAAFEARDWP VTVRLSKIRI GLYTACIDQL VPLLKAGLPE LTTDEQLWPT 130 140 150 160 170 180 AEAELLAAIE GRYEADFAFA FWQSLRRKLV SDEWRPVSYD AGSTARRTTS PAAVLKTTAT 190 200 210 220 230 240 TLPITAEVIA GILDEAGFRV PWRDRDGDAA LAAQAIETAL EPLSPRPGEP VKIEIADSGF 250 260 270 280 290 300 LRNRGACLVG RIKLRDRGDM PMRNLPLLIA LLNEKDGLVV DAVLTDSDEL QYAFSSTLAN 310 320 330 340 350 360 YHATNPRYHE LARLLYELMP KRPLGTQYSC IGFHHLGKVA VMSEILAEHR KTKEKLATAP 370 380 390 400 410 420 GFKGTVAIAF TMPSSAYVLK IIRDHPTDDY KFDYFDGLDE VLRKYNLVHE IDRAGSMLDN 430 440 450 460 470 480 IIYSNVKLDR AMFAPELLDE LLEAGIGTVT LDRGALVFRH LIVQIKLTPL PLYLANASAA 490 500 510 520 530 540 ESRAAVINLG DCIKNNAAAD IFNKDLDGRN YGVSRIRKVY LFDYDAVEPL TSVTVSRDGA 550 560 570 580 590 600 APGEFDNGMV FRPQEMLEGL RIDDPGLRRA FRDAHPELMQ ADYWEGMQQA LRDGKVPKVM 610 NYPASRRLRR

References

[1]

"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris." Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S. Nat. Biotechnol. 22:55-61(2004) [PubMed: 14704707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX572594 Genomic DNA. Translation: CAE25791.1.
RefSeq	NP_945700.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2689655.
GenomeReviews	Gene locus RPA0347 in contig BX571963_GR.
KEGG	rpa:RPA0347.
NMPDR	fig|258594.1.peg.344.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6NCX0.
OMA	FYRDFLH.
Enzyme and pathway databases
BioCyc	RPAL258594:RPA0347-MON.
BRENDA	2.7.11.5. 373.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_RHOPA

Accession

Primary (citable) accession number: Q6NCX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)