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Q6NCM9 (TRMB_RHOPA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:RPA0442
OrganismRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) [Complete proteome] [HAMAP]
Taxonomic identifier258594 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas

Protein attributes

Sequence length265 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 265265tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171383

Sites

Active site1701 By similarity
Binding site961S-adenosyl-L-methionine By similarity
Binding site1211S-adenosyl-L-methionine By similarity
Binding site1481S-adenosyl-L-methionine By similarity
Binding site1701S-adenosyl-L-methionine By similarity
Binding site1741Substrate By similarity
Binding site2061Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6NCM9 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 9C78AAE665729330

FASTA26530,230
        10         20         30         40         50         60 
MAVLYAGRPV QKCERSRLMF NIDQPDSAPD NEDHDGDVVM HGQGSFFGRR KGHKLRAHQA 

        70         80         90        100        110        120 
DLIENLLPHL SLQIDSPAPE PLTTLFDPPV EHMRLEIGFG GGEHLIAEAL AHPDTGFIGA 

       130        140        150        160        170        180 
EPYVNGMAKI LARIEAENIR NIRLFAGDAS ELLAWVPAGS LARIDLIHPD PWPKRRHWKR 

       190        200        210        220        230        240 
RFVQDATVAA MARALTPHGE FRFVCDIDGY TAWTLAHLLR APCFDWLAQR ADDWRKPWPN 

       250        260 
YTMTRYGRKA EREGRRANYL RFERL 

« Hide

References

[1]"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris."
Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S.
Nat. Biotechnol. 22:55-61(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX572594 Genomic DNA. Translation: CAE25886.1.
RefSeqNP_945795.1. NC_005296.1.

3D structure databases

ProteinModelPortalQ6NCM9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING258594.RPA0442.

Proteomic databases

PRIDEQ6NCM9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE25886; CAE25886; RPA0442.
GeneID2689452.
KEGGrpa:RPA0442.
PATRIC23285043. VBIRhoPal84835_0469.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMAIGCEPFV.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_RHOPA
AccessionPrimary (citable) accession number: Q6NCM9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways