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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway:iN(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961S-adenosyl-L-methionineUniRule annotation
Binding sitei121 – 1211S-adenosyl-L-methionineUniRule annotation
Binding sitei148 – 1481S-adenosyl-L-methionineUniRule annotation
Active sitei170 – 1701By similarity
Binding sitei170 – 1701S-adenosyl-L-methionineUniRule annotation
Binding sitei174 – 1741SubstrateUniRule annotation
Binding sitei206 – 2061SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:RPA0442
OrganismiRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Taxonomic identifieri258594 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
ProteomesiUP000001426 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 265265tRNA (guanine-N(7)-)-methyltransferasePRO_0000171383Add
BLAST

Proteomic databases

PRIDEiQ6NCM9.

Interactioni

Protein-protein interaction databases

STRINGi258594.RPA0442.

Structurei

3D structure databases

ProteinModelPortaliQ6NCM9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OMAiIGCEPFV.
OrthoDBiEOG6K6VBC.
PhylomeDBiQ6NCM9.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NCM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVLYAGRPV QKCERSRLMF NIDQPDSAPD NEDHDGDVVM HGQGSFFGRR
60 70 80 90 100
KGHKLRAHQA DLIENLLPHL SLQIDSPAPE PLTTLFDPPV EHMRLEIGFG
110 120 130 140 150
GGEHLIAEAL AHPDTGFIGA EPYVNGMAKI LARIEAENIR NIRLFAGDAS
160 170 180 190 200
ELLAWVPAGS LARIDLIHPD PWPKRRHWKR RFVQDATVAA MARALTPHGE
210 220 230 240 250
FRFVCDIDGY TAWTLAHLLR APCFDWLAQR ADDWRKPWPN YTMTRYGRKA
260
EREGRRANYL RFERL
Length:265
Mass (Da):30,230
Last modified:July 5, 2004 - v1
Checksum:i9C78AAE665729330
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572594 Genomic DNA. Translation: CAE25886.1.
RefSeqiWP_011156010.1. NC_005296.1.

Genome annotation databases

EnsemblBacteriaiCAE25886; CAE25886; RPA0442.
KEGGirpa:RPA0442.
PATRICi23285043. VBIRhoPal84835_0469.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572594 Genomic DNA. Translation: CAE25886.1.
RefSeqiWP_011156010.1. NC_005296.1.

3D structure databases

ProteinModelPortaliQ6NCM9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi258594.RPA0442.

Proteomic databases

PRIDEiQ6NCM9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE25886; CAE25886; RPA0442.
KEGGirpa:RPA0442.
PATRICi23285043. VBIRhoPal84835_0469.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OMAiIGCEPFV.
OrthoDBiEOG6K6VBC.
PhylomeDBiQ6NCM9.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-98 / CGA009.

Entry informationi

Entry nameiTRMB_RHOPA
AccessioniPrimary (citable) accession number: Q6NCM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: July 22, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.