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Q6NC62 (GLO2_RHOPA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6
Alternative name(s):
Glyoxalase II
Short name=Glx II
Gene names
Name:gloB
Ordered Locus Names:RPA0610
OrganismRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) [Complete proteome] [HAMAP]
Taxonomic identifier258594 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length255 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374

Catalytic activity

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374

Cofactor

Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374

Pathway

Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374

Subunit structure

Monomer By similarity. HAMAP MF_01374

Sequence similarities

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular functionhydroxyacylglutathione hydrolase activity

Inferred from electronic annotation. Source: EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 255255Hydroxyacylglutathione hydrolase HAMAP MF_01374
PRO_0000309693

Sites

Metal binding561Zinc 1 By similarity
Metal binding581Zinc 1 By similarity
Metal binding601Zinc 2 By similarity
Metal binding611Zinc 2 By similarity
Metal binding1141Zinc 1 By similarity
Metal binding1331Zinc 1 By similarity
Metal binding1331Zinc 2 By similarity
Metal binding1711Zinc 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6NC62 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 4263C6DDE6A134D1

FASTA25527,574
        10         20         30         40         50         60 
MPADIRIVPC LTDNFGYLVH DPATGATASI DAPEAAPLIA ALDKEGWKLT DILVTHHHGD 

        70         80         90        100        110        120 
HVGGIAELKK KYQCRVHAPH DANAKIADAD VRLQEGDVVR VGDLTARVLE TPGHTLDHLS 

       130        140        150        160        170        180 
YVFDDDRALF AADTLFSIGC GRVFEGTYPM MWESLLKLRA LPDDFKLYCG HEYTASNVKF 

       190        200        210        220        230        240 
ALGIEPDNAA LQARAKQVES LRAEGKPTIP VTLGEEKQAN VFLRADVPSV AAAVGMPGAP 

       250 
AAEVFGEIRE RKNNG

« Hide

References

[1]"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris."
Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S.
Nat. Biotechnol. 22:55-61(2004) [PubMed: 14704707] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX572594 Genomic DNA. Translation: CAE26054.1.
RefSeqNP_945963.1. NC_005296.1.

3D structure databases

HSSPHSSP built from PDB template 1XM8 based on UniProtKB Q9SID3.
ProteinModelPortalQ6NC62.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2692749.
GenomeReviewsGene locus RPA0610 in contig BX571963_GR.
KEGGrpa:RPA0610.
NMPDRfig|258594.1.peg.607.
PATRIC23285397. VBIRhoPal84835_0644.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG753931.
OMAIGCGRVF.
ProtClustDBCLSK818037.

Enzyme and pathway databases

BioCycRPAL258594:RPA0610-MONOMER.

Family and domain databases

HAMAPMF_01374. Glyoxalase_2.
[Tree]
InterProIPR001279. Beta-lactamas-like.
IPR017782. Hydroxyacylglutathione_Hdrlase.
[Graphical view]
KOK01069.
PANTHERPTHR11935:SF7. PTHR11935:SF7. 1 hit.
PfamPF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTSM00849. Lactamase_B. 1 hit.
[Graphical view]
TIGRFAMsTIGR03413. GSH_gloB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLO2_RHOPA
AccessionPrimary (citable) accession number: Q6NC62
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: July 5, 2004
Last modified: January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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