Q6NAM5 (LPXK_RHOPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	RPA1159

Organism

Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) [Complete proteome] [HAMAP]

Taxonomic identifier

258594 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_0000190943
Regions
	Nucleotide binding	51 – 58	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6NAM5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 32450902CBC6F0B5	FASTA	340	35,595
	10 20 30 40 50 60 MREPGFWHRP PSLVSRLLLP IAAIYGNIAA ARMQKAGTTV GVPVLCVGNY HMGGAGKTPT 70 80 90 100 110 120 TLALVALLRE FGETPVVLSR GYGGRLQGPV QVDPSRHSAA DIGDEPLMMA RRVPVVVARD 130 140 150 160 170 180 RTDGAALACA LGATVILMDD GFQNPALTKD ASLIVVDSHR SIGNGSVFPA GPLRAPLPLQ 190 200 210 220 230 240 VARTDALVVV GDGTAADGLA QQITTKGGVV LRARLVPEPA SVEALRGRRV LAFAGIGDPA 250 260 270 280 290 300 RFVATLRDSG VEVVEQRAFA DHHPFTAEEL AELAAAAKRD GLTLVTTEKD LARIGGAQQA 310 320 330 340 LGVEIVPFAV TLAFGDEAKL RLFLLDRLNG ARAAKLAGRR

References

[1]

"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris." Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S. Nat. Biotechnol. 22:55-61(2004) [PubMed: 14704707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX572596 Genomic DNA. Translation: CAE26602.1.
RefSeq	NP_946510.1. NC_005296.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2691625.
GenomeReviews	Gene locus RPA1159 in contig BX571963_GR.
KEGG	rpa:RPA1159.
NMPDR	fig|258594.1.peg.1154.
PATRIC	23286541. VBIRhoPal84835_1211.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	PRK00652.
Enzyme and pathway databases
BioCyc	RPAL258594:RPA1159-MONOMER.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_RHOPA

Accession

Primary (citable) accession number: Q6NAM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program