Reviewed, UniProtKB/Swiss-Prot Q6N9J7 (ACSF_RHOPA)

Names and origin

Protein names

Recommended name:
    Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Aerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	acsF
Ordered Locus Names:	RPA1552

Organism

Rhodopseudomonas palustris [Complete proteome] [HAMAP]

Taxonomic identifier

1076 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. HAMAP MF_01840 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. HAMAP MF_01840 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O. HAMAP MF_01840
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; bacteriochlorophyll biosynthesis (light-independent). HAMAP MF_01840
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Bacteriochlorophyll biosynthesis Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	bacteriochlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase HAMAP MF_01840		PRO_0000217534

Sequences

	Sequence		Length	Mass (Da)
	Q6N9J7-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 4D6E38C1AA35ED7B	FASTA	365	42,483
	10 20 30 40 50 60 MIPMEGGAQG ALRTRPDIKG SVDSLNIAKQ DTILTPRFYT TDYAAMDKLD VSLVRAEWTA 70 80 90 100 110 120 MMNELRADYN KSHFKKTDEF VNSDLDKLPP ELRAEFKDFL VSSLTAEFSG CVLYAEIKKR 130 140 150 160 170 180 IKNPEIRELF GLLSRDEARH AGFINEILKD HGIGVDLSFL TKVKKYTYFR PKFIFYATYL 190 200 210 220 230 240 SEKIGYARYI TIYRQMERHP ERRFHPIFKW FERWCNDEFR HGEAFALLMR ADPSLLSGVN 250 260 270 280 290 300 KLWIRFFLLA VFSTMYVRDH MRPAFYEALG VDATDYGMQV FRITTEISKQ VFPVTINLDD 310 320 330 340 350 360 PRFLQNLERL RIAAEKIDRS HSQGLLGKLK RPFYAASAAL AFGRLFLLPA KRNELPRVIG LRPAW

References

[1]

"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris." Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S. Nat. Biotechnol. 22:55-61(2004) [PubMed: 14704707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX572597 Genomic DNA. Translation: CAE26993.1.
RefSeq	NP_946898.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2691628.
GenomeReviews	Gene locus RPA1552 in contig BX571963_GR.
KEGG	rpa:RPA1552.
NMPDR	fig|258594.1.peg.1542.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6N9J7.
OMA	Q6N9J7. TAEFSGC.
Enzyme and pathway databases
BioCyc	RPAL258594:RPA1552-MON.
BRENDA	1.14.13.81. 373.
Family and domain databases
HAMAP	MF_01840. [Tree]
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_RHOPA

Accession

Primary (citable) accession number: Q6N9J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 5, 2004
Last modified:	May 5, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)