Reviewed, UniProtKB/Swiss-Prot Q6N8V0 (DNAE2_RHOPA)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	RPA1803

Organism

Rhodopseudomonas palustris [Complete proteome] [HAMAP]

Taxonomic identifier

1076 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	1195 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: InterPro error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1195	1195	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103398

Sequences

	Sequence		Length	Mass (Da)
	Q6N8V0-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1D1F8E9803B1E1D0	FASTA	1,195	133,397
	10 20 30 40 50 60 MSARYAEIGV TTNFSFLEGG SHPQDYVHEA SRLGLDAIGI ADRNTLAGVV RAFSEFDNEE 70 80 90 100 110 120 LIHKPKLLIG TRLCFADGTP DLLAYPTDRA AYGRLCRLLS AGKLRAGKGE CHLTFADLEA 130 140 150 160 170 180 FIHERSPSIH ELATVIHPPS ASINQPSASI HQFPTPSWPG LTRPSTPTSK VVLVSVDARV 190 200 210 220 230 240 KPGHDGGKRV ADNSQILLVV MPSYRFQTKT IAIALERLTA LNADAVWLGL TPYYRGDDKR 250 260 270 280 290 300 RLARLRRIAA VARVPCIATN DVLYHHPRRR ALQDVLTCVR EKTSIDKAGR RLEGNAERHL 310 320 330 340 350 360 KPAAEMARLF RHDPEAIAET LRFADRISFT LDELKYHYPD EPVPPGKTAQ AHLQDLTEQG 370 380 390 400 410 420 IKQYFPNGIS DRLRATIAKE LAIIERRGYA HYFLTVHDIV RYARSQDILC QGRGSAANSA 430 440 450 460 470 480 VCYVLGITCV DPTEIDLLFE RFVSEERDEP PDIDVDFEHS RREEVMQYIY RRYGRHRAAI 490 500 510 520 530 540 VATVIHYRPR SAIRDVGKAL GLSEDVTAAL ADTVWGSWGK GLNEMQVRQA GLDPHNPMIG 550 560 570 580 590 600 RAVELATELI GFPRHLSQHV GGYVLTQDRL DSYVPIGNAA MADRTFIEWD KDDIDAVKMM 610 620 630 640 650 660 KVDVLALGML TCIRKGFHLI AQHKGVRFQL SDIKSEDDNA VYKMLQRGES IGVFQVESRA 670 680 690 700 710 720 QMNMLPRLKP RCFYDLVIEV AIVRPGPIQG DMVHPYLRRR NGQEPVVYPS PSGEAGDKNE 730 740 750 760 770 780 LRQILGKTLG VPLFQEQAMR IAIEAAHFTP DEANQLRRAM ATFRNVGTIG KFEAKMIGNL 790 800 810 820 830 840 VKRGYDATFA KNCFEQIKGF GSYGFPESHA ASFAKLVYVS AWMKCEHPDA FCCALLNSQP 850 860 870 880 890 900 MGFYAPAQIV GDARSNGVEV RPVDVSFSEG QCTLEERCGR HHAVRLGFRM IDGFRWADPD 910 920 930 940 950 960 EERVQLEAGE PPSDDWAARI VAARARGAFT SLEQFARITA LPKRALILLA DADAFRSLGL 970 980 990 1000 1010 1020 DRRAALWAVR RLPDDVPLPL FEAASAREQE DERAAPLPAM PMAEHVVADY QTVRLSLKGH 1030 1040 1050 1060 1070 1080 PMEFLRALFA AERVVTCREV SRSEQRASGC WLRCAGVVLV RQRPGSANGV IFMTIEDETG 1090 1100 1110 1120 1130 1140 IANIVVWPAV MEKYRKEVMG ARLVLVEGKV QASAEGVVHL VAERLIDRSA EMGRLAEGLV 1150 1160 1170 1180 1190 RPALPDGADL YEPLTSEKYN YEALNGDRRD TPDAPAQRHR HPRDVRILPP SRDFH

References

[1]

"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris." Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S. Nat. Biotechnol. 22:55-61(2004) [PubMed: 14704707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX572598 Genomic DNA. Translation: CAE27244.1.
RefSeq	NP_947148.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2692528.
GenomeReviews	Gene locus RPA1803 in contig BX571963_GR.
KEGG	rpa:RPA1803.
NMPDR	fig|258594.1.peg.1792.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6N8V0.
Enzyme and pathway databases
BioCyc	RPAL258594:RPA1803-MON.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_RHOPA

Accession

Primary (citable) accession number: Q6N8V0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)