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Protein

Cytochrome p450 CYP199A2

Gene

cyp199a2

Organism
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The oxidative demethylation of 4-methoxybenzoate requires the participation of the monooxygenase CYP199A2, the ferredoxin-like protein ThcC/RPA1872 and a ferredoxin reductase to mediate the transfer of electrons from NADH to CYP199A2. It is also active with 4-ethylbenzoate.2 Publications

Catalytic activityi

4-methoxybenzoate + AH2 + O2 = 4-hydroxybenzoate + formaldehyde + A + H2O.2 Publications

Cofactori

heme3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei247Substrate1 Publication1
Metal bindingi361Iron (heme axial ligand)2 Publications1

GO - Molecular functioni

  • 4-methoxybenzoate monooxygenase (O-demethylating) activity Source: UniProtKB
  • heme binding Source: UniProtKB
  • iron ion binding Source: UniProtKB

GO - Biological processi

  • oxidation-reduction process Source: UniProtKB
  • xenobiotic metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.99.15. 5412.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome p450 CYP199A21 Publication (EC:1.14.99.152 Publications)
Alternative name(s):
4-methoxybenzoate O-demethylase CYP199A2Curated
4-methoxybenzoate monooxygenase (O-demethylating)Curated
Gene namesi
Name:cyp199a21 Publication
Ordered Locus Names:RPA1871Imported
OrganismiRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)Imported
Taxonomic identifieri258594 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
Proteomesi
  • UP000001426 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004306731 – 412Cytochrome p450 CYP199A2Add BLAST412

Interactioni

Subunit structurei

Interacts with the ferredoxin-like iron-sulfur protein ThcC.1 Publication

Protein-protein interaction databases

STRINGi258594.RPA1871.

Structurei

Secondary structure

1412
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 34Combined sources5
Helixi37 – 46Combined sources10
Beta strandi48 – 52Combined sources5
Turni53 – 56Combined sources4
Beta strandi57 – 60Combined sources4
Helixi63 – 70Combined sources8
Turni73 – 75Combined sources3
Beta strandi76 – 78Combined sources3
Beta strandi82 – 85Combined sources4
Turni87 – 89Combined sources3
Turni98 – 101Combined sources4
Helixi106 – 117Combined sources12
Helixi120 – 143Combined sources24
Beta strandi146 – 149Combined sources4
Helixi151 – 156Combined sources6
Helixi157 – 167Combined sources11
Helixi174 – 176Combined sources3
Helixi177 – 187Combined sources11
Helixi193 – 200Combined sources8
Helixi203 – 212Combined sources10
Helixi215 – 217Combined sources3
Helixi223 – 229Combined sources7
Turni230 – 234Combined sources5
Helixi240 – 251Combined sources12
Helixi254 – 269Combined sources16
Helixi271 – 279Combined sources9
Helixi281 – 283Combined sources3
Helixi284 – 295Combined sources12
Beta strandi300 – 307Combined sources8
Beta strandi309 – 311Combined sources3
Beta strandi314 – 316Combined sources3
Beta strandi321 – 325Combined sources5
Helixi326 – 329Combined sources4
Turni333 – 335Combined sources3
Beta strandi336 – 338Combined sources3
Helixi364 – 381Combined sources18
Beta strandi382 – 387Combined sources6
Beta strandi392 – 395Combined sources4
Beta strandi397 – 404Combined sources8
Beta strandi406 – 411Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FR7X-ray2.01A1-412[»]
4DNJX-ray1.80A1-412[»]
ProteinModelPortaliQ6N8N2.
SMRiQ6N8N2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6N8N2.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 97Substrate binding1 Publication4

Sequence similaritiesi

Belongs to the cytochrome P450 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105Q56. Bacteria.
ENOG410XT87. LUCA.
HOGENOMiHOG000243678.
OMAiSGIHMCV.
OrthoDBiPOG091H0API.
PhylomeDBiQ6N8N2.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6N8N2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTAPSLVPV TTPSQHGAGV PHLGIDPFAL DYFADPYPEQ ETLREAGPVV
60 70 80 90 100
YLDKWNVYGV ARYAEVYAVL NDPLTFCSSR GVGLSDFKKE KPWRPPSLIL
110 120 130 140 150
EADPPAHTRT RAVLSKVLSP ATMKRLRDGF AAAADAKIDE LLARGGNIDA
160 170 180 190 200
IADLAEAYPL SVFPDAMGLK QEGRENLLPY AGLVFNAFGP PNELRQSAIE
210 220 230 240 250
RSAPHQAYVA EQCQRPNLAP GGFGACIHAF SDTGEITPEE APLLVRSLLS
260 270 280 290 300
AGLDTTVNGI AAAVYCLARF PDEFARLRAD PSLARNAFEE AVRFESPVQT
310 320 330 340 350
FFRTTTRDVE LAGATIGEGE KVLMFLGSAN RDPRRWDDPD RYDITRKTSG
360 370 380 390 400
HVGFGSGVHM CVGQLVARLE GEVVLAALAR KVAAIEIAGP LKRRFNNTLR
410
GLESLPIQLT PA
Length:412
Mass (Da):44,623
Last modified:July 5, 2004 - v1
Checksum:i71E0DEA6E28EA89B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572599 Genomic DNA. Translation: CAE27312.1.
RefSeqiWP_011157377.1. NC_005296.1.

Genome annotation databases

EnsemblBacteriaiCAE27312; CAE27312; RPA1871.
KEGGirpa:RPA1871.
PATRICi23288039. VBIRhoPal84835_1956.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572599 Genomic DNA. Translation: CAE27312.1.
RefSeqiWP_011157377.1. NC_005296.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FR7X-ray2.01A1-412[»]
4DNJX-ray1.80A1-412[»]
ProteinModelPortaliQ6N8N2.
SMRiQ6N8N2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi258594.RPA1871.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE27312; CAE27312; RPA1871.
KEGGirpa:RPA1871.
PATRICi23288039. VBIRhoPal84835_1956.

Phylogenomic databases

eggNOGiENOG4105Q56. Bacteria.
ENOG410XT87. LUCA.
HOGENOMiHOG000243678.
OMAiSGIHMCV.
OrthoDBiPOG091H0API.
PhylomeDBiQ6N8N2.

Enzyme and pathway databases

BRENDAi1.14.99.15. 5412.

Miscellaneous databases

EvolutionaryTraceiQ6N8N2.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYPA2_RHOPA
AccessioniPrimary (citable) accession number: Q6N8N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 29, 2014
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.