Reviewed, UniProtKB/Swiss-Prot Q6N622 (GLYA2_RHOPA)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 2
      Short name=Serine methylase 2
      Short name=SHMT 2
    EC=2.1.2.1

Gene names

Name:	glyA2
Ordered Locus Names:	RPA2796

Organism

Rhodopseudomonas palustris [Complete proteome] [HAMAP]

Taxonomic identifier

1076 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity. HAMAP MF_00051
Subcellular location	Cytoplasm By similarity HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Serine hydroxymethyltransferase 2 HAMAP MF_00051		PRO_0000113652
Amino acid modifications
	Modified residue	245	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6N622-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 3477D211B1B1EE1C	FASTA	434	45,360
	10 20 30 40 50 60 MGPSVAPHAV HTIANPGVSA AGLVAADHAV AAAIADEETR QRDSIELIAS ENFVSRAVLD 70 80 90 100 110 120 AQGSVLTNKY AEGYPHRRYY GGCANVDAIE DLVIARVNQL FGSAYANVQP HSGSQANQAV 130 140 150 160 170 180 FLALLAPGDT ILGLDLKAGG HLTHGAPVNM SGRWFKAVSY GVDPETHRID MDQVAVQARQ 190 200 210 220 230 240 HRPRLLIAGG SAYPRIIDFG RFRQIADEVG AILMVDMAHF AGLVAGGVYP SPVPFADVVT 250 260 270 280 290 300 STTHKTLRGP RGGFVLTNDA NIAKKINSAT FPGLQGGPLM HVIAAKAVAF GEALQPEFGA 310 320 330 340 350 360 YAQAVVENCR VLAQALADGG LTITSGGTDC HLAVVDLRPF GVTGNIAEQA LESVGITLNK 370 380 390 400 410 420 NAIPNDPEKP MVTSGIRVGT AAGTSRGFGA DQYREIAGLV LETLHAVRAG TLDAAGQEIN 430 KSVRRLAASF PLPY

References

[1]

"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris." Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S. Nat. Biotechnol. 22:55-61(2004) [PubMed: 14704707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX572602 Genomic DNA. Translation: CAE28238.1.
RefSeq	NP_948139.1.
3D structure databases
SMR	Q6N622. Positions 27-419.
ModBase	Search...
Genome annotation databases
GeneID	2693327.
KEGG	rpa:RPA2796.
NMPDR	fig|258594.1.peg.2782.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG301263.
OMA	VTMSGKW.
Enzyme and pathway databases
BioCyc	RPAL258594:RPA2796-MONOMER.
BRENDA	2.1.2.1. 373.
Family and domain databases
HAMAP	MF_00051_B. SHMT_B. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA2_RHOPA

Accession

Primary (citable) accession number: Q6N622

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)